Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycdG and b1007 are separated by 20 nucleotides b1007 and ycdI are separated by 10 nucleotides ycdI and rarA are separated by 9 nucleotides
b1006: ycdG - putative transport protein (VIMSS), at 1,067,734 to 1,069,062
ycdG
b1007: b1007 - orf, hypothetical protein (VIMSS), at 1,069,083 to 1,069,577
b1007
b1008: ycdI - hypothetical protein (NCBI), at 1,069,588 to 1,070,178
ycdI
b1009: rarA - predicted hydrolase (NCBI), at 1,070,188 to 1,070,988
rarA
Position (kb)
1069
1070 Strain fitness (log2 ratio)
-1
0
1
2 at 1068.096 kb on - strand, within ycdG at 1068.544 kb on - strand, within ycdG at 1068.547 kb on + strand, within ycdG at 1068.622 kb on + strand, within ycdG at 1068.622 kb on + strand, within ycdG at 1068.702 kb on + strand, within ycdG at 1068.702 kb on + strand, within ycdG at 1068.921 kb on - strand, within ycdG at 1068.921 kb on - strand, within ycdG at 1068.970 kb on - strand at 1068.999 kb on + strand at 1069.014 kb on + strand at 1069.046 kb on + strand at 1069.046 kb on + strand at 1069.126 kb on - strand at 1069.242 kb on + strand, within b1007 at 1069.600 kb on + strand at 1069.660 kb on + strand, within ycdI at 1069.662 kb on - strand, within ycdI at 1069.662 kb on - strand, within ycdI at 1069.797 kb on + strand, within ycdI at 1069.797 kb on + strand, within ycdI at 1069.870 kb on - strand, within ycdI at 1069.870 kb on - strand, within ycdI at 1069.872 kb on + strand, within ycdI at 1069.872 kb on + strand at 1070.145 kb on - strand at 1070.145 kb on - strand at 1070.152 kb on - strand at 1070.241 kb on - strand at 1070.241 kb on - strand at 1070.569 kb on - strand, within rarA
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 1,068,096 - ycdG b1006 0.27 -0.4 1,068,544 - ycdG b1006 0.61 +0.3 1,068,547 + ycdG b1006 0.61 -0.2 1,068,622 + ycdG b1006 0.67 +0.7 1,068,622 + ycdG b1006 0.67 +1.7 1,068,702 + ycdG b1006 0.73 -0.0 1,068,702 + ycdG b1006 0.73 -0.9 1,068,921 - ycdG b1006 0.89 +0.7 1,068,921 - ycdG b1006 0.89 +0.7 1,068,970 - +2.3 1,068,999 + -0.7 1,069,014 + +0.7 1,069,046 + +0.3 1,069,046 + -0.3 1,069,126 - -0.2 1,069,242 + b1007 b1007 0.32 -1.2 1,069,600 + +0.6 1,069,660 + ycdI b1008 0.12 -0.2 1,069,662 - ycdI b1008 0.13 +1.3 1,069,662 - ycdI b1008 0.13 -1.1 1,069,797 + ycdI b1008 0.35 -0.5 1,069,797 + ycdI b1008 0.35 -0.0 1,069,870 - ycdI b1008 0.48 -0.7 1,069,870 - ycdI b1008 0.48 -1.1 1,069,872 + ycdI b1008 0.48 -0.3 1,069,872 + +0.7 1,070,145 - +0.5 1,070,145 - -0.0 1,070,152 - -0.3 1,070,241 - +0.7 1,070,241 - +1.1 1,070,569 - rarA b1009 0.48 +1.5
Or see this region's nucleotide sequence