Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycbJ and ycbC overlap by 24 nucleotides ycbC and smtA are separated by 135 nucleotides smtA and mukF overlap by 4 nucleotides
b0919: ycbJ - hypothetical protein (NCBI), at 970,975 to 971,868
ycbJ
b0920: ycbC - conserved inner membrane protein (NCBI), at 971,845 to 972,624
ycbC
b0921: smtA - predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI), at 972,760 to 973,545
smtA
b0922: mukF - condesin subunit F (NCBI), at 973,542 to 974,864
mukF
Position (kb) 971
972
973 Strain fitness (log2 ratio) -2
-1
0
1
2 at 970.868 kb on - strand at 970.868 kb on - strand at 971.000 kb on + strand at 971.061 kb on + strand at 971.071 kb on + strand, within ycbJ at 971.221 kb on - strand, within ycbJ at 971.291 kb on + strand, within ycbJ at 971.291 kb on + strand, within ycbJ at 971.300 kb on + strand, within ycbJ at 971.300 kb on + strand, within ycbJ at 971.348 kb on - strand, within ycbJ at 971.348 kb on - strand, within ycbJ at 971.350 kb on + strand, within ycbJ at 971.382 kb on + strand, within ycbJ at 971.382 kb on + strand, within ycbJ at 971.426 kb on + strand, within ycbJ at 971.434 kb on - strand, within ycbJ at 971.436 kb on + strand, within ycbJ at 971.442 kb on + strand, within ycbJ at 971.442 kb on + strand, within ycbJ at 971.447 kb on + strand, within ycbJ at 971.447 kb on + strand, within ycbJ at 971.447 kb on + strand, within ycbJ at 971.706 kb on + strand, within ycbJ at 972.023 kb on - strand, within ycbC at 972.065 kb on - strand, within ycbC at 972.215 kb on - strand, within ycbC at 972.313 kb on - strand, within ycbC at 972.351 kb on - strand, within ycbC at 972.354 kb on + strand, within ycbC at 972.354 kb on + strand, within ycbC at 972.535 kb on + strand, within ycbC at 972.548 kb on - strand at 972.819 kb on + strand at 972.852 kb on + strand, within smtA at 972.985 kb on + strand, within smtA at 973.035 kb on - strand, within smtA at 973.035 kb on - strand, within smtA at 973.068 kb on + strand, within smtA at 973.068 kb on + strand, within smtA at 973.113 kb on - strand, within smtA at 973.128 kb on - strand, within smtA at 973.166 kb on + strand, within smtA at 973.169 kb on - strand, within smtA at 973.228 kb on - strand, within smtA at 973.228 kb on - strand, within smtA at 973.307 kb on - strand, within smtA at 973.381 kb on - strand, within smtA at 973.397 kb on - strand, within smtA at 973.416 kb on - strand, within smtA at 973.416 kb on - strand, within smtA at 973.431 kb on + strand, within smtA at 973.507 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 970,868 - -0.1 970,868 - -0.2 971,000 + -0.6 971,061 + -0.9 971,071 + ycbJ b0919 0.11 +2.7 971,221 - ycbJ b0919 0.28 +0.6 971,291 + ycbJ b0919 0.35 +0.1 971,291 + ycbJ b0919 0.35 +0.7 971,300 + ycbJ b0919 0.36 -1.4 971,300 + ycbJ b0919 0.36 -0.9 971,348 - ycbJ b0919 0.42 +0.1 971,348 - ycbJ b0919 0.42 -0.3 971,350 + ycbJ b0919 0.42 -0.5 971,382 + ycbJ b0919 0.46 +0.7 971,382 + ycbJ b0919 0.46 +0.7 971,426 + ycbJ b0919 0.50 +1.0 971,434 - ycbJ b0919 0.51 +0.7 971,436 + ycbJ b0919 0.52 +1.1 971,442 + ycbJ b0919 0.52 -1.9 971,442 + ycbJ b0919 0.52 +0.9 971,447 + ycbJ b0919 0.53 -0.9 971,447 + ycbJ b0919 0.53 +0.3 971,447 + ycbJ b0919 0.53 +0.5 971,706 + ycbJ b0919 0.82 +1.9 972,023 - ycbC b0920 0.23 -1.0 972,065 - ycbC b0920 0.28 -1.0 972,215 - ycbC b0920 0.47 -1.4 972,313 - ycbC b0920 0.60 -0.6 972,351 - ycbC b0920 0.65 -1.9 972,354 + ycbC b0920 0.65 -0.3 972,354 + ycbC b0920 0.65 -0.3 972,535 + ycbC b0920 0.88 -0.5 972,548 - -0.7 972,819 + -0.6 972,852 + smtA b0921 0.12 -0.4 972,985 + smtA b0921 0.29 -0.1 973,035 - smtA b0921 0.35 -0.6 973,035 - smtA b0921 0.35 -1.4 973,068 + smtA b0921 0.39 +0.3 973,068 + smtA b0921 0.39 +1.0 973,113 - smtA b0921 0.45 -0.7 973,128 - smtA b0921 0.47 -0.6 973,166 + smtA b0921 0.52 +0.0 973,169 - smtA b0921 0.52 -1.3 973,228 - smtA b0921 0.60 -0.3 973,228 - smtA b0921 0.60 +0.4 973,307 - smtA b0921 0.70 -0.2 973,381 - smtA b0921 0.79 -0.9 973,397 - smtA b0921 0.81 -1.0 973,416 - smtA b0921 0.83 -0.7 973,416 - smtA b0921 0.83 -1.8 973,431 + smtA b0921 0.85 -0.2 973,507 - -1.2
Or see this region's nucleotide sequence