Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dsbG and ahpC are separated by 371 nucleotides ahpC and ahpF are separated by 244 nucleotides
b0604: dsbG - thiol:disulfide interchange protein (VIMSS), at 637,050 to 637,796
dsbG
b0605: ahpC - alkyl hydroperoxide reductase, C22 subunit (NCBI), at 638,168 to 638,731
ahpC
b0606: ahpF - alkyl hydroperoxide reductase, F52a subunit; detoxification of hydroperoxides (VIMSS), at 638,976 to 640,541
ahpF
Position (kb) 638
639 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 637.185 kb on + strand, within dsbG at 637.204 kb on - strand, within dsbG at 637.204 kb on - strand, within dsbG at 637.217 kb on + strand, within dsbG at 637.237 kb on - strand, within dsbG at 637.237 kb on - strand, within dsbG at 637.244 kb on + strand, within dsbG at 637.498 kb on - strand, within dsbG at 637.836 kb on + strand at 637.944 kb on + strand at 637.944 kb on + strand at 637.969 kb on + strand at 638.144 kb on + strand at 638.144 kb on + strand at 638.315 kb on + strand, within ahpC at 638.376 kb on - strand, within ahpC at 638.378 kb on + strand, within ahpC at 638.489 kb on - strand, within ahpC at 638.513 kb on + strand, within ahpC at 638.513 kb on + strand, within ahpC at 638.557 kb on + strand, within ahpC at 638.557 kb on + strand, within ahpC at 638.557 kb on + strand, within ahpC at 638.625 kb on + strand, within ahpC at 638.692 kb on + strand at 638.923 kb on - strand at 638.923 kb on - strand at 638.937 kb on - strand at 639.002 kb on + strand at 639.227 kb on - strand, within ahpF at 639.227 kb on - strand, within ahpF at 639.268 kb on - strand, within ahpF at 639.428 kb on - strand, within ahpF at 639.448 kb on - strand, within ahpF at 639.639 kb on - strand, within ahpF at 639.681 kb on + strand, within ahpF
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 637,185 + dsbG b0604 0.18 +0.3 637,204 - dsbG b0604 0.21 -1.4 637,204 - dsbG b0604 0.21 -0.7 637,217 + dsbG b0604 0.22 +0.3 637,237 - dsbG b0604 0.25 -0.6 637,237 - dsbG b0604 0.25 +0.8 637,244 + dsbG b0604 0.26 +1.4 637,498 - dsbG b0604 0.60 -0.8 637,836 + +0.1 637,944 + -1.0 637,944 + +2.9 637,969 + +0.4 638,144 + -2.2 638,144 + -0.2 638,315 + ahpC b0605 0.26 -1.7 638,376 - ahpC b0605 0.37 -2.1 638,378 + ahpC b0605 0.37 -0.1 638,489 - ahpC b0605 0.57 -0.9 638,513 + ahpC b0605 0.61 +0.0 638,513 + ahpC b0605 0.61 +0.3 638,557 + ahpC b0605 0.69 -0.5 638,557 + ahpC b0605 0.69 -2.2 638,557 + ahpC b0605 0.69 -1.8 638,625 + ahpC b0605 0.81 +0.4 638,692 + -1.6 638,923 - -0.2 638,923 - +1.3 638,937 - +0.8 639,002 + +0.8 639,227 - ahpF b0606 0.16 +0.8 639,227 - ahpF b0606 0.16 -0.4 639,268 - ahpF b0606 0.19 +0.8 639,428 - ahpF b0606 0.29 -0.5 639,448 - ahpF b0606 0.30 -1.1 639,639 - ahpF b0606 0.42 -1.3 639,681 + ahpF b0606 0.45 +0.2
Or see this region's nucleotide sequence