Strain Fitness in Escherichia coli BW25113 around b0184

Experiment: Cisplatin 0.05 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlpxB and rnhB overlap by 4 nucleotidesrnhB and dnaE are separated by 36 nucleotidesdnaE and accA are separated by 12 nucleotides b0182: lpxB - lipid-A-disaccharide synthase (NCBI), at 203,348 to 204,496 lpxB b0183: rnhB - ribonuclease HII (NCBI), at 204,493 to 205,089 rnhB b0184: dnaE - DNA polymerase III subunit alpha (NCBI), at 205,126 to 208,608 dnaE b0185: accA - acetyl-CoA carboxylase subunit alpha (NCBI), at 208,621 to 209,580 accA Position (kb) 205 206 207 208 209Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 204.573 kb on + strand, within rnhBat 204.697 kb on + strand, within rnhBat 204.705 kb on - strand, within rnhBat 204.705 kb on - strand, within rnhBat 204.721 kb on + strand, within rnhBat 204.832 kb on + strand, within rnhBat 204.832 kb on + strand, within rnhBat 205.038 kb on - strandat 205.049 kb on + strandat 205.068 kb on - strandat 205.092 kb on + strandat 208.591 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml
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204,573 + rnhB b0183 0.13 +2.6
204,697 + rnhB b0183 0.34 -0.8
204,705 - rnhB b0183 0.36 -1.1
204,705 - rnhB b0183 0.36 -2.3
204,721 + rnhB b0183 0.38 -1.9
204,832 + rnhB b0183 0.57 +0.5
204,832 + rnhB b0183 0.57 -2.3
205,038 - -2.1
205,049 + -0.0
205,068 - -3.3
205,092 + +1.1
208,591 + -0.9

Or see this region's nucleotide sequence