Experiment: Cisplatin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfjK and yfjL overlap by 4 nucleotides yfjL and yfjM are separated by 359 nucleotides yfjM and yfjN are separated by 141 nucleotides
b2627: yfjK - CP4-57 prophage; conserved protein (NCBI), at 2,759,373 to 2,761,562
yfjK
b2628: yfjL - CP4-57 prophage; predicted protein (NCBI), at 2,761,559 to 2,763,175
yfjL
b2629: yfjM - CP4-57 prophage; predicted protein (NCBI), at 2,763,535 to 2,763,798
yfjM
b2630: yfjN - CP4-57 prophage; RNase LS (NCBI), at 2,763,940 to 2,765,013
yfjN
Position (kb)
2761
2762
2763
2764 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2760.572 kb on + strand, within yfjK at 2760.587 kb on + strand, within yfjK at 2760.681 kb on - strand, within yfjK at 2760.803 kb on - strand, within yfjK at 2760.862 kb on - strand, within yfjK at 2760.862 kb on - strand, within yfjK at 2760.947 kb on - strand, within yfjK at 2760.990 kb on + strand, within yfjK at 2760.990 kb on + strand, within yfjK at 2761.107 kb on + strand, within yfjK at 2761.188 kb on + strand, within yfjK at 2761.211 kb on - strand, within yfjK at 2761.240 kb on + strand, within yfjK at 2761.297 kb on - strand, within yfjK at 2761.312 kb on - strand, within yfjK at 2761.312 kb on - strand, within yfjK at 2761.319 kb on + strand, within yfjK at 2761.454 kb on - strand at 2761.454 kb on - strand at 2762.010 kb on + strand, within yfjL at 2762.039 kb on - strand, within yfjL at 2762.218 kb on + strand, within yfjL at 2762.263 kb on - strand, within yfjL at 2762.272 kb on + strand, within yfjL at 2762.274 kb on - strand, within yfjL at 2762.313 kb on + strand, within yfjL at 2762.313 kb on + strand, within yfjL at 2762.316 kb on + strand, within yfjL at 2762.316 kb on + strand, within yfjL at 2762.323 kb on - strand, within yfjL at 2762.391 kb on - strand, within yfjL at 2762.405 kb on - strand, within yfjL at 2762.405 kb on - strand, within yfjL at 2762.500 kb on + strand, within yfjL at 2762.500 kb on + strand, within yfjL at 2762.545 kb on + strand, within yfjL at 2762.545 kb on + strand, within yfjL at 2762.555 kb on - strand, within yfjL at 2762.555 kb on - strand, within yfjL at 2762.626 kb on + strand, within yfjL at 2762.860 kb on + strand, within yfjL at 2762.878 kb on + strand, within yfjL at 2762.881 kb on - strand, within yfjL at 2762.881 kb on - strand, within yfjL at 2762.891 kb on - strand, within yfjL at 2762.918 kb on + strand, within yfjL at 2762.982 kb on + strand, within yfjL at 2763.052 kb on - strand at 2763.059 kb on + strand at 2763.074 kb on - strand at 2763.104 kb on - strand at 2763.119 kb on + strand at 2763.137 kb on + strand at 2763.173 kb on + strand at 2763.265 kb on - strand at 2763.265 kb on - strand at 2763.365 kb on + strand at 2763.411 kb on + strand at 2763.445 kb on + strand at 2763.445 kb on + strand at 2763.561 kb on - strand at 2763.561 kb on - strand at 2763.588 kb on - strand, within yfjM at 2763.611 kb on - strand, within yfjM at 2763.621 kb on - strand, within yfjM at 2763.642 kb on + strand, within yfjM at 2763.650 kb on - strand, within yfjM at 2763.652 kb on + strand, within yfjM at 2763.758 kb on + strand, within yfjM at 2763.758 kb on + strand, within yfjM at 2763.833 kb on + strand at 2763.833 kb on + strand at 2763.836 kb on + strand at 2763.836 kb on + strand at 2763.841 kb on - strand at 2763.844 kb on - strand at 2763.844 kb on - strand at 2763.851 kb on + strand at 2763.921 kb on + strand at 2764.043 kb on + strand at 2764.082 kb on + strand, within yfjN at 2764.093 kb on + strand, within yfjN at 2764.093 kb on + strand, within yfjN
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.025 mg/ml remove 2,760,572 + yfjK b2627 0.55 -0.1 2,760,587 + yfjK b2627 0.55 +2.1 2,760,681 - yfjK b2627 0.60 +0.1 2,760,803 - yfjK b2627 0.65 -0.3 2,760,862 - yfjK b2627 0.68 -0.3 2,760,862 - yfjK b2627 0.68 -0.6 2,760,947 - yfjK b2627 0.72 -0.5 2,760,990 + yfjK b2627 0.74 -0.5 2,760,990 + yfjK b2627 0.74 -0.2 2,761,107 + yfjK b2627 0.79 +0.1 2,761,188 + yfjK b2627 0.83 +0.4 2,761,211 - yfjK b2627 0.84 -0.4 2,761,240 + yfjK b2627 0.85 -0.8 2,761,297 - yfjK b2627 0.88 +2.1 2,761,312 - yfjK b2627 0.89 +0.3 2,761,312 - yfjK b2627 0.89 +0.9 2,761,319 + yfjK b2627 0.89 +0.5 2,761,454 - +0.7 2,761,454 - +0.4 2,762,010 + yfjL b2628 0.28 +1.1 2,762,039 - yfjL b2628 0.30 +0.3 2,762,218 + yfjL b2628 0.41 +0.9 2,762,263 - yfjL b2628 0.44 +0.1 2,762,272 + yfjL b2628 0.44 -1.4 2,762,274 - yfjL b2628 0.44 +0.6 2,762,313 + yfjL b2628 0.47 +0.5 2,762,313 + yfjL b2628 0.47 +0.7 2,762,316 + yfjL b2628 0.47 +1.3 2,762,316 + yfjL b2628 0.47 +2.3 2,762,323 - yfjL b2628 0.47 +0.3 2,762,391 - yfjL b2628 0.51 +1.3 2,762,405 - yfjL b2628 0.52 +0.7 2,762,405 - yfjL b2628 0.52 -1.2 2,762,500 + yfjL b2628 0.58 +1.2 2,762,500 + yfjL b2628 0.58 -0.6 2,762,545 + yfjL b2628 0.61 -0.1 2,762,545 + yfjL b2628 0.61 -0.3 2,762,555 - yfjL b2628 0.62 +0.7 2,762,555 - yfjL b2628 0.62 -0.1 2,762,626 + yfjL b2628 0.66 +0.7 2,762,860 + yfjL b2628 0.80 +0.1 2,762,878 + yfjL b2628 0.82 +1.4 2,762,881 - yfjL b2628 0.82 +0.8 2,762,881 - yfjL b2628 0.82 +0.2 2,762,891 - yfjL b2628 0.82 +0.4 2,762,918 + yfjL b2628 0.84 +1.1 2,762,982 + yfjL b2628 0.88 -0.3 2,763,052 - +0.7 2,763,059 + +0.5 2,763,074 - +0.5 2,763,104 - -0.0 2,763,119 + +0.4 2,763,137 + +0.0 2,763,173 + +0.5 2,763,265 - -2.7 2,763,265 - -1.9 2,763,365 + -0.0 2,763,411 + -0.4 2,763,445 + -0.2 2,763,445 + -0.4 2,763,561 - -2.0 2,763,561 - -2.3 2,763,588 - yfjM b2629 0.20 -2.2 2,763,611 - yfjM b2629 0.29 -3.4 2,763,621 - yfjM b2629 0.33 -0.3 2,763,642 + yfjM b2629 0.41 -0.4 2,763,650 - yfjM b2629 0.44 -0.3 2,763,652 + yfjM b2629 0.44 -0.1 2,763,758 + yfjM b2629 0.84 -0.1 2,763,758 + yfjM b2629 0.84 +0.6 2,763,833 + +0.3 2,763,833 + +1.0 2,763,836 + -0.3 2,763,836 + +0.5 2,763,841 - -0.4 2,763,844 - -1.6 2,763,844 - -0.3 2,763,851 + -0.9 2,763,921 + -0.2 2,764,043 + +0.2 2,764,082 + yfjN b2630 0.13 -0.5 2,764,093 + yfjN b2630 0.14 +0.3 2,764,093 + yfjN b2630 0.14 -0.7
Or see this region's nucleotide sequence