Experiment: Cisplatin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hcaC and hcaB overlap by 4 nucleotides hcaB and hcaD are separated by 9 nucleotides hcaD and yphA are separated by 96 nucleotides yphA and yphB are separated by 47 nucleotides
b2540: hcaC - 3-phenylpropionate dioxygenase, predicted ferredoxin subunit (NCBI), at 2,668,930 to 2,669,250
hcaC
b2541: hcaB - 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase (NCBI), at 2,669,247 to 2,670,059
hcaB
b2542: hcaD - phenylpropionate dioxygenase, ferredoxin reductase subunit (NCBI), at 2,670,069 to 2,671,271
hcaD
b2543: yphA - predicted inner membrane protein (RefSeq), at 2,671,368 to 2,671,790
yphA
b2544: yphB - hypothetical protein (NCBI), at 2,671,838 to 2,672,710
yphB
Position (kb)
2670
2671
2672 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2669.083 kb on + strand, within hcaC at 2669.112 kb on - strand, within hcaC at 2669.166 kb on + strand, within hcaC at 2669.295 kb on + strand at 2669.442 kb on - strand, within hcaB at 2669.469 kb on - strand at 2669.662 kb on + strand, within hcaB at 2669.678 kb on - strand, within hcaB at 2669.678 kb on - strand, within hcaB at 2669.716 kb on - strand, within hcaB at 2669.716 kb on - strand, within hcaB at 2669.827 kb on + strand, within hcaB at 2669.827 kb on + strand, within hcaB at 2669.835 kb on - strand, within hcaB at 2669.835 kb on - strand, within hcaB at 2669.946 kb on + strand, within hcaB at 2669.947 kb on - strand, within hcaB at 2669.951 kb on + strand, within hcaB at 2669.951 kb on + strand, within hcaB at 2670.153 kb on + strand at 2670.199 kb on + strand, within hcaD at 2670.427 kb on - strand, within hcaD at 2670.427 kb on - strand, within hcaD at 2670.447 kb on + strand, within hcaD at 2670.450 kb on - strand, within hcaD at 2670.450 kb on - strand, within hcaD at 2670.576 kb on - strand, within hcaD at 2670.576 kb on - strand, within hcaD at 2670.576 kb on - strand, within hcaD at 2670.603 kb on + strand, within hcaD at 2670.836 kb on - strand, within hcaD at 2670.927 kb on - strand, within hcaD at 2670.994 kb on - strand, within hcaD at 2670.994 kb on - strand, within hcaD at 2671.037 kb on + strand, within hcaD at 2671.203 kb on + strand at 2671.315 kb on + strand at 2671.315 kb on + strand at 2671.315 kb on + strand at 2671.323 kb on - strand at 2671.373 kb on - strand at 2671.373 kb on - strand at 2671.494 kb on + strand, within yphA at 2671.494 kb on + strand, within yphA at 2671.496 kb on + strand, within yphA at 2671.497 kb on - strand, within yphA at 2671.502 kb on - strand, within yphA at 2671.502 kb on - strand, within yphA at 2671.509 kb on + strand, within yphA at 2671.603 kb on + strand, within yphA at 2671.641 kb on - strand, within yphA at 2671.645 kb on - strand, within yphA at 2671.713 kb on + strand, within yphA at 2671.736 kb on + strand, within yphA at 2671.736 kb on + strand, within yphA at 2671.789 kb on + strand at 2671.890 kb on - strand at 2671.959 kb on - strand, within yphB at 2672.031 kb on - strand, within yphB at 2672.031 kb on - strand, within yphB at 2672.053 kb on + strand, within yphB at 2672.081 kb on + strand, within yphB at 2672.084 kb on - strand, within yphB at 2672.165 kb on - strand, within yphB at 2672.165 kb on - strand, within yphB
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.025 mg/ml remove 2,669,083 + hcaC b2540 0.48 +1.1 2,669,112 - hcaC b2540 0.57 +0.5 2,669,166 + hcaC b2540 0.74 +0.6 2,669,295 + -0.7 2,669,442 - hcaB b2541 0.24 -0.7 2,669,469 - -0.3 2,669,662 + hcaB b2541 0.51 +0.6 2,669,678 - hcaB b2541 0.53 -0.9 2,669,678 - hcaB b2541 0.53 -0.3 2,669,716 - hcaB b2541 0.58 -2.3 2,669,716 - hcaB b2541 0.58 +0.1 2,669,827 + hcaB b2541 0.71 +1.1 2,669,827 + hcaB b2541 0.71 -0.7 2,669,835 - hcaB b2541 0.72 -0.3 2,669,835 - hcaB b2541 0.72 +0.7 2,669,946 + hcaB b2541 0.86 +0.7 2,669,947 - hcaB b2541 0.86 +0.2 2,669,951 + hcaB b2541 0.87 +0.7 2,669,951 + hcaB b2541 0.87 +0.4 2,670,153 + +0.5 2,670,199 + hcaD b2542 0.11 +0.4 2,670,427 - hcaD b2542 0.30 -0.4 2,670,427 - hcaD b2542 0.30 +0.7 2,670,447 + hcaD b2542 0.31 -1.1 2,670,450 - hcaD b2542 0.32 +0.3 2,670,450 - hcaD b2542 0.32 +0.3 2,670,576 - hcaD b2542 0.42 +0.6 2,670,576 - hcaD b2542 0.42 -0.5 2,670,576 - hcaD b2542 0.42 -0.9 2,670,603 + hcaD b2542 0.44 -0.1 2,670,836 - hcaD b2542 0.64 +0.6 2,670,927 - hcaD b2542 0.71 -0.7 2,670,994 - hcaD b2542 0.77 +0.5 2,670,994 - hcaD b2542 0.77 +0.5 2,671,037 + hcaD b2542 0.80 -0.3 2,671,203 + +2.0 2,671,315 + +0.3 2,671,315 + +1.3 2,671,315 + +1.0 2,671,323 - -1.4 2,671,373 - +0.7 2,671,373 - +1.3 2,671,494 + yphA b2543 0.30 +0.2 2,671,494 + yphA b2543 0.30 +0.7 2,671,496 + yphA b2543 0.30 +0.2 2,671,497 - yphA b2543 0.30 +0.2 2,671,502 - yphA b2543 0.32 +0.7 2,671,502 - yphA b2543 0.32 +1.3 2,671,509 + yphA b2543 0.33 -1.7 2,671,603 + yphA b2543 0.56 -1.6 2,671,641 - yphA b2543 0.65 +0.6 2,671,645 - yphA b2543 0.65 -0.3 2,671,713 + yphA b2543 0.82 -0.1 2,671,736 + yphA b2543 0.87 +2.3 2,671,736 + yphA b2543 0.87 -0.7 2,671,789 + +0.5 2,671,890 - +0.6 2,671,959 - yphB b2544 0.14 +0.5 2,672,031 - yphB b2544 0.22 -0.0 2,672,031 - yphB b2544 0.22 +0.5 2,672,053 + yphB b2544 0.25 +0.8 2,672,081 + yphB b2544 0.28 -0.9 2,672,084 - yphB b2544 0.28 +0.3 2,672,165 - yphB b2544 0.37 +0.0 2,672,165 - yphB b2544 0.37 +0.3
Or see this region's nucleotide sequence