Strain Fitness in Escherichia coli BW25113 around b1749

Experiment: Cisplatin 0.025 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntastC and xthA are separated by 445 nucleotidesxthA and ydjX are separated by 166 nucleotidesydjX and ydjY are separated by 4 nucleotides b1748: astC - succinylornithine transaminase, PLP-dependent (NCBI), at 1,828,786 to 1,830,006 astC b1749: xthA - exonuclease III (NCBI), at 1,830,452 to 1,831,258 xthA b1750: ydjX - orf, hypothetical protein (VIMSS), at 1,831,425 to 1,832,135 ydjX b1751: ydjY - orf, hypothetical protein (VIMSS), at 1,832,140 to 1,832,817 ydjY Position (kb) 1830 1831 1832Strain fitness (log2 ratio) -2 -1 0 1 2at 1829.492 kb on - strand, within astCat 1829.492 kb on - strand, within astCat 1829.911 kb on - strandat 1829.911 kb on - strandat 1829.941 kb on - strandat 1830.075 kb on + strandat 1830.078 kb on + strandat 1830.078 kb on + strandat 1830.093 kb on - strandat 1830.238 kb on - strandat 1830.238 kb on - strandat 1830.375 kb on + strandat 1830.422 kb on - strandat 1830.640 kb on - strand, within xthAat 1830.904 kb on + strand, within xthAat 1830.904 kb on + strand, within xthAat 1830.979 kb on + strand, within xthAat 1831.114 kb on + strand, within xthAat 1831.114 kb on + strand, within xthAat 1831.119 kb on + strand, within xthAat 1831.119 kb on + strand, within xthAat 1831.243 kb on - strandat 1831.243 kb on - strandat 1831.277 kb on - strandat 1831.277 kb on - strandat 1831.351 kb on + strandat 1831.351 kb on + strandat 1831.504 kb on - strand, within ydjXat 1831.535 kb on - strand, within ydjXat 1831.535 kb on - strand, within ydjXat 1831.539 kb on - strand, within ydjXat 1831.642 kb on + strand, within ydjXat 1831.658 kb on + strand, within ydjXat 1831.679 kb on - strand, within ydjXat 1831.737 kb on - strand, within ydjXat 1831.737 kb on - strand, within ydjXat 1831.766 kb on + strand, within ydjXat 1831.766 kb on + strand, within ydjXat 1831.775 kb on + strand, within ydjXat 1831.782 kb on + strand, within ydjXat 1831.827 kb on - strand, within ydjXat 1831.834 kb on + strand, within ydjXat 1831.992 kb on - strand, within ydjXat 1832.016 kb on + strand, within ydjXat 1832.016 kb on + strand, within ydjXat 1832.084 kb on - strandat 1832.113 kb on - strandat 1832.113 kb on - strandat 1832.187 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.025 mg/ml
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1,829,492 - astC b1748 0.58 -0.2
1,829,492 - astC b1748 0.58 +0.9
1,829,911 - -0.4
1,829,911 - +0.3
1,829,941 - -0.1
1,830,075 + -0.2
1,830,078 + -0.8
1,830,078 + -0.7
1,830,093 - +1.1
1,830,238 - -0.7
1,830,238 - +0.3
1,830,375 + +0.9
1,830,422 - -0.2
1,830,640 - xthA b1749 0.23 -0.7
1,830,904 + xthA b1749 0.56 +0.3
1,830,904 + xthA b1749 0.56 +0.3
1,830,979 + xthA b1749 0.65 -0.9
1,831,114 + xthA b1749 0.82 -1.3
1,831,114 + xthA b1749 0.82 -2.7
1,831,119 + xthA b1749 0.83 -1.0
1,831,119 + xthA b1749 0.83 +0.0
1,831,243 - -1.5
1,831,243 - +0.1
1,831,277 - +0.3
1,831,277 - +0.3
1,831,351 + +0.3
1,831,351 + +1.9
1,831,504 - ydjX b1750 0.11 -0.0
1,831,535 - ydjX b1750 0.15 -0.2
1,831,535 - ydjX b1750 0.15 +0.9
1,831,539 - ydjX b1750 0.16 -0.2
1,831,642 + ydjX b1750 0.31 +0.7
1,831,658 + ydjX b1750 0.33 +0.3
1,831,679 - ydjX b1750 0.36 -0.1
1,831,737 - ydjX b1750 0.44 +0.2
1,831,737 - ydjX b1750 0.44 +1.2
1,831,766 + ydjX b1750 0.48 -1.5
1,831,766 + ydjX b1750 0.48 -0.0
1,831,775 + ydjX b1750 0.49 +0.3
1,831,782 + ydjX b1750 0.50 +0.3
1,831,827 - ydjX b1750 0.57 -0.7
1,831,834 + ydjX b1750 0.58 +0.8
1,831,992 - ydjX b1750 0.80 +0.7
1,832,016 + ydjX b1750 0.83 -0.1
1,832,016 + ydjX b1750 0.83 -0.1
1,832,084 - +0.2
1,832,113 - -1.1
1,832,113 - -0.7
1,832,187 + -0.2

Or see this region's nucleotide sequence