Experiment: Cisplatin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bioB and bioF overlap by 4 nucleotides bioF and bioC overlap by 14 nucleotides bioC and bioD overlap by 8 nucleotides
b0775: bioB - biotin synthase (NCBI), at 808,567 to 809,607
bioB
b0776: bioF - 8-amino-7-oxononanoate synthase (NCBI), at 809,604 to 810,758
bioF
b0777: bioC - predicted methltransferase, enzyme of biotin synthesis (NCBI), at 810,745 to 811,500
bioC
b0778: bioD - dethiobiotin synthetase (NCBI), at 811,493 to 812,170
bioD
Position (kb) 809
810
811 Strain fitness (log2 ratio) -2
-1
0
1 at 808.684 kb on + strand, within bioB at 808.904 kb on - strand, within bioB at 808.915 kb on + strand, within bioB at 808.949 kb on + strand, within bioB at 808.964 kb on + strand, within bioB at 809.074 kb on + strand, within bioB at 809.082 kb on - strand, within bioB at 809.217 kb on - strand, within bioB at 809.217 kb on - strand, within bioB at 809.233 kb on + strand, within bioB at 809.379 kb on + strand, within bioB at 809.723 kb on + strand, within bioF at 809.993 kb on + strand, within bioF at 809.993 kb on + strand, within bioF at 810.237 kb on + strand, within bioF at 810.237 kb on + strand, within bioF at 810.359 kb on + strand, within bioF at 810.362 kb on - strand, within bioF at 810.393 kb on - strand, within bioF at 810.414 kb on + strand, within bioF at 810.492 kb on + strand, within bioF at 810.492 kb on + strand, within bioF at 810.673 kb on + strand at 810.673 kb on - strand at 810.729 kb on + strand at 810.791 kb on + strand at 810.833 kb on + strand, within bioC at 810.971 kb on + strand, within bioC at 811.122 kb on + strand, within bioC at 811.122 kb on + strand, within bioC at 811.122 kb on + strand, within bioC at 811.122 kb on + strand, within bioC at 811.326 kb on + strand, within bioC at 811.326 kb on + strand, within bioC at 811.326 kb on + strand, within bioC at 811.366 kb on - strand, within bioC at 811.423 kb on + strand, within bioC at 811.423 kb on + strand, within bioC at 811.431 kb on - strand at 811.446 kb on - strand at 811.446 kb on - strand at 811.464 kb on - strand at 811.559 kb on + strand at 811.672 kb on + strand, within bioD at 811.720 kb on - strand, within bioD at 811.757 kb on + strand, within bioD
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.025 mg/ml remove 808,684 + bioB b0775 0.11 -0.4 808,904 - bioB b0775 0.32 -0.6 808,915 + bioB b0775 0.33 +0.2 808,949 + bioB b0775 0.37 +0.5 808,964 + bioB b0775 0.38 -0.2 809,074 + bioB b0775 0.49 +0.6 809,082 - bioB b0775 0.49 +1.1 809,217 - bioB b0775 0.62 -0.4 809,217 - bioB b0775 0.62 -1.0 809,233 + bioB b0775 0.64 -0.4 809,379 + bioB b0775 0.78 -0.7 809,723 + bioF b0776 0.10 +0.2 809,993 + bioF b0776 0.34 -2.0 809,993 + bioF b0776 0.34 +0.6 810,237 + bioF b0776 0.55 -0.5 810,237 + bioF b0776 0.55 +0.8 810,359 + bioF b0776 0.65 +0.2 810,362 - bioF b0776 0.66 +0.0 810,393 - bioF b0776 0.68 -0.1 810,414 + bioF b0776 0.70 +0.6 810,492 + bioF b0776 0.77 -0.2 810,492 + bioF b0776 0.77 -0.4 810,673 + +0.0 810,673 - +0.9 810,729 + +1.2 810,791 + +0.7 810,833 + bioC b0777 0.12 +0.6 810,971 + bioC b0777 0.30 +0.4 811,122 + bioC b0777 0.50 +0.5 811,122 + bioC b0777 0.50 +1.4 811,122 + bioC b0777 0.50 +0.6 811,122 + bioC b0777 0.50 +0.6 811,326 + bioC b0777 0.77 +0.1 811,326 + bioC b0777 0.77 +0.4 811,326 + bioC b0777 0.77 +1.4 811,366 - bioC b0777 0.82 +1.0 811,423 + bioC b0777 0.90 -1.2 811,423 + bioC b0777 0.90 +0.3 811,431 - +0.0 811,446 - +0.2 811,446 - +0.0 811,464 - +0.5 811,559 + -2.6 811,672 + bioD b0778 0.26 +0.2 811,720 - bioD b0778 0.33 +0.3 811,757 + bioD b0778 0.39 -0.2
Or see this region's nucleotide sequence