Experiment: m.b. Spectinomycin 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0719 and Echvi_0720 are separated by 255 nucleotides Echvi_0720 and Echvi_0721 are separated by 274 nucleotides Echvi_0721 and Echvi_0722 are separated by 5 nucleotides
Echvi_0719: Echvi_0719 - pseudouridine synthase, at 810,728 to 811,873
_0719
Echvi_0720: Echvi_0720 - hypothetical protein, at 812,129 to 812,263
_0720
Echvi_0721: Echvi_0721 - Uncharacterized protein conserved in bacteria, at 812,538 to 812,819
_0721
Echvi_0722: Echvi_0722 - Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes, at 812,825 to 814,201
_0722
Position (kb) 812
813 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 811.895 kb on - strand at 811.897 kb on - strand at 811.906 kb on + strand at 811.907 kb on - strand at 811.913 kb on - strand at 811.944 kb on + strand at 811.964 kb on - strand at 812.056 kb on - strand at 812.221 kb on + strand, within Echvi_0720 at 812.222 kb on - strand, within Echvi_0720 at 812.268 kb on + strand at 812.270 kb on + strand at 812.270 kb on + strand at 812.351 kb on - strand at 812.372 kb on - strand at 812.411 kb on - strand at 812.411 kb on - strand at 812.514 kb on + strand at 812.524 kb on + strand at 812.525 kb on - strand at 812.537 kb on + strand at 812.537 kb on + strand at 812.538 kb on - strand at 812.607 kb on + strand, within Echvi_0721 at 812.642 kb on - strand, within Echvi_0721 at 812.746 kb on - strand, within Echvi_0721 at 812.781 kb on + strand, within Echvi_0721 at 812.817 kb on + strand at 812.877 kb on + strand at 813.172 kb on + strand, within Echvi_0722 at 813.173 kb on - strand, within Echvi_0722 at 813.236 kb on - strand, within Echvi_0722 at 813.240 kb on + strand, within Echvi_0722 at 813.302 kb on + strand, within Echvi_0722 at 813.302 kb on + strand, within Echvi_0722 at 813.302 kb on + strand, within Echvi_0722 at 813.302 kb on + strand, within Echvi_0722 at 813.303 kb on - strand, within Echvi_0722 at 813.307 kb on + strand, within Echvi_0722 at 813.308 kb on - strand, within Echvi_0722 at 813.308 kb on - strand, within Echvi_0722 at 813.308 kb on - strand, within Echvi_0722 at 813.372 kb on - strand, within Echvi_0722 at 813.548 kb on + strand, within Echvi_0722 at 813.562 kb on + strand, within Echvi_0722 at 813.600 kb on + strand, within Echvi_0722 at 813.601 kb on - strand, within Echvi_0722 at 813.654 kb on + strand, within Echvi_0722 at 813.740 kb on + strand, within Echvi_0722 at 813.740 kb on + strand, within Echvi_0722 at 813.741 kb on - strand, within Echvi_0722 at 813.741 kb on - strand, within Echvi_0722 at 813.741 kb on - strand, within Echvi_0722 at 813.741 kb on - strand, within Echvi_0722 at 813.741 kb on - strand, within Echvi_0722 at 813.788 kb on + strand, within Echvi_0722
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.12 mg/ml remove 811,895 - +0.1 811,897 - -0.0 811,906 + +0.6 811,907 - -0.2 811,913 - +0.3 811,944 + -1.9 811,964 - -0.4 812,056 - -0.9 812,221 + Echvi_0720 0.68 -0.4 812,222 - Echvi_0720 0.69 -0.1 812,268 + -0.5 812,270 + +0.1 812,270 + -0.4 812,351 - -0.1 812,372 - +0.4 812,411 - +0.5 812,411 - -0.1 812,514 + +0.1 812,524 + -0.4 812,525 - -0.6 812,537 + +0.1 812,537 + +0.3 812,538 - -0.4 812,607 + Echvi_0721 0.24 -0.2 812,642 - Echvi_0721 0.37 -1.9 812,746 - Echvi_0721 0.74 -1.4 812,781 + Echvi_0721 0.86 -0.4 812,817 + -0.1 812,877 + +1.5 813,172 + Echvi_0722 0.25 -0.1 813,173 - Echvi_0722 0.25 +0.7 813,236 - Echvi_0722 0.30 +0.7 813,240 + Echvi_0722 0.30 +2.9 813,302 + Echvi_0722 0.35 -0.8 813,302 + Echvi_0722 0.35 -0.8 813,302 + Echvi_0722 0.35 +0.7 813,302 + Echvi_0722 0.35 -0.4 813,303 - Echvi_0722 0.35 -0.3 813,307 + Echvi_0722 0.35 +0.5 813,308 - Echvi_0722 0.35 -0.3 813,308 - Echvi_0722 0.35 -0.6 813,308 - Echvi_0722 0.35 -0.7 813,372 - Echvi_0722 0.40 -0.4 813,548 + Echvi_0722 0.53 -0.9 813,562 + Echvi_0722 0.54 +1.5 813,600 + Echvi_0722 0.56 -0.6 813,601 - Echvi_0722 0.56 -0.0 813,654 + Echvi_0722 0.60 -1.8 813,740 + Echvi_0722 0.66 +0.1 813,740 + Echvi_0722 0.66 +0.9 813,741 - Echvi_0722 0.67 +0.9 813,741 - Echvi_0722 0.67 -0.5 813,741 - Echvi_0722 0.67 +0.2 813,741 - Echvi_0722 0.67 -1.3 813,741 - Echvi_0722 0.67 -0.4 813,788 + Echvi_0722 0.70 -0.2
Or see this region's nucleotide sequence