Experiment: m.b. Spectinomycin 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0662 and Echvi_0663 are separated by 143 nucleotides Echvi_0663 and Echvi_0664 are separated by 104 nucleotides Echvi_0664 and Echvi_0665 are separated by 46 nucleotides
Echvi_0662: Echvi_0662 - Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily), at 745,351 to 746,112
_0662
Echvi_0663: Echvi_0663 - Predicted methyltransferases, at 746,256 to 746,969
_0663
Echvi_0664: Echvi_0664 - Methylase involved in ubiquinone/menaquinone biosynthesis, at 747,074 to 747,772
_0664
Echvi_0665: Echvi_0665 - FOG: Transposase and inactivated derivatives, at 747,819 to 749,051
_0665
Position (kb) 747
748 Strain fitness (log2 ratio) -1
0
1
2 at 746.208 kb on - strand at 746.274 kb on + strand at 746.274 kb on + strand at 746.361 kb on + strand, within Echvi_0663 at 746.362 kb on - strand, within Echvi_0663 at 746.397 kb on - strand, within Echvi_0663 at 746.454 kb on - strand, within Echvi_0663 at 746.723 kb on + strand, within Echvi_0663 at 746.723 kb on + strand, within Echvi_0663 at 746.723 kb on + strand, within Echvi_0663 at 746.991 kb on - strand at 747.003 kb on + strand at 747.003 kb on + strand at 747.004 kb on - strand at 747.007 kb on + strand at 747.007 kb on + strand at 747.008 kb on - strand at 747.008 kb on - strand at 747.051 kb on + strand at 747.157 kb on + strand, within Echvi_0664 at 747.159 kb on + strand, within Echvi_0664 at 747.160 kb on - strand, within Echvi_0664 at 747.160 kb on - strand, within Echvi_0664 at 747.210 kb on + strand, within Echvi_0664 at 747.210 kb on + strand, within Echvi_0664 at 747.211 kb on - strand, within Echvi_0664 at 747.211 kb on - strand, within Echvi_0664 at 747.234 kb on + strand, within Echvi_0664 at 747.234 kb on + strand, within Echvi_0664 at 747.235 kb on - strand, within Echvi_0664 at 747.235 kb on - strand, within Echvi_0664 at 747.289 kb on + strand, within Echvi_0664 at 747.427 kb on + strand, within Echvi_0664 at 747.427 kb on + strand, within Echvi_0664 at 747.427 kb on + strand, within Echvi_0664 at 747.427 kb on + strand, within Echvi_0664 at 747.428 kb on - strand, within Echvi_0664 at 747.428 kb on - strand, within Echvi_0664 at 747.450 kb on + strand, within Echvi_0664 at 747.451 kb on - strand, within Echvi_0664 at 747.537 kb on + strand, within Echvi_0664 at 747.538 kb on - strand, within Echvi_0664 at 747.613 kb on - strand, within Echvi_0664 at 747.613 kb on - strand, within Echvi_0664 at 747.613 kb on - strand, within Echvi_0664 at 747.659 kb on + strand, within Echvi_0664 at 747.659 kb on + strand, within Echvi_0664 at 747.671 kb on + strand, within Echvi_0664 at 747.671 kb on + strand, within Echvi_0664 at 747.728 kb on + strand at 747.729 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.12 mg/ml remove 746,208 - -0.4 746,274 + -0.8 746,274 + +1.4 746,361 + Echvi_0663 0.15 -1.0 746,362 - Echvi_0663 0.15 +0.9 746,397 - Echvi_0663 0.20 -1.1 746,454 - Echvi_0663 0.28 +0.2 746,723 + Echvi_0663 0.65 -0.5 746,723 + Echvi_0663 0.65 +0.2 746,723 + Echvi_0663 0.65 +1.5 746,991 - -0.3 747,003 + -1.7 747,003 + +2.1 747,004 - +0.4 747,007 + -0.5 747,007 + -0.8 747,008 - -0.5 747,008 - +0.2 747,051 + +0.5 747,157 + Echvi_0664 0.12 +0.3 747,159 + Echvi_0664 0.12 +1.0 747,160 - Echvi_0664 0.12 -0.6 747,160 - Echvi_0664 0.12 +1.9 747,210 + Echvi_0664 0.19 +0.2 747,210 + Echvi_0664 0.19 +0.5 747,211 - Echvi_0664 0.20 +0.0 747,211 - Echvi_0664 0.20 -0.3 747,234 + Echvi_0664 0.23 -0.7 747,234 + Echvi_0664 0.23 +0.5 747,235 - Echvi_0664 0.23 +0.9 747,235 - Echvi_0664 0.23 +1.1 747,289 + Echvi_0664 0.31 +0.7 747,427 + Echvi_0664 0.51 -1.0 747,427 + Echvi_0664 0.51 +0.8 747,427 + Echvi_0664 0.51 +1.9 747,427 + Echvi_0664 0.51 +0.9 747,428 - Echvi_0664 0.51 +0.5 747,428 - Echvi_0664 0.51 -0.9 747,450 + Echvi_0664 0.54 +0.7 747,451 - Echvi_0664 0.54 +0.4 747,537 + Echvi_0664 0.66 -0.1 747,538 - Echvi_0664 0.66 +0.9 747,613 - Echvi_0664 0.77 +1.9 747,613 - Echvi_0664 0.77 +0.1 747,613 - Echvi_0664 0.77 +0.2 747,659 + Echvi_0664 0.84 +1.2 747,659 + Echvi_0664 0.84 -0.9 747,671 + Echvi_0664 0.85 -0.7 747,671 + Echvi_0664 0.85 -0.7 747,728 + -0.2 747,729 - -0.8
Or see this region's nucleotide sequence