Experiment: m.b. Spectinomycin 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0103 and Echvi_0104 are separated by 104 nucleotides Echvi_0104 and Echvi_0105 are separated by 18 nucleotides Echvi_0105 and Echvi_0106 are separated by 43 nucleotides Echvi_0106 and Echvi_0107 are separated by 8 nucleotides Echvi_0107 and Echvi_0108 are separated by 2 nucleotides
Echvi_0103: Echvi_0103 - hypothetical protein, at 101,012 to 101,533
_0103
Echvi_0104: Echvi_0104 - Iron-sulfur cluster assembly accessory protein, at 101,638 to 101,958
_0104
Echvi_0105: Echvi_0105 - hypothetical protein, at 101,977 to 102,477
_0105
Echvi_0106: Echvi_0106 - hypothetical protein, at 102,521 to 102,982
_0106
Echvi_0107: Echvi_0107 - dihydroneopterin aldolase, at 102,991 to 103,356
_0107
Echvi_0108: Echvi_0108 - DivIVA domain, at 103,359 to 104,243
_0108
Position (kb) 102
103 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 101.536 kb on - strand at 101.538 kb on + strand at 101.559 kb on + strand at 101.587 kb on + strand at 101.588 kb on - strand at 101.629 kb on + strand at 101.652 kb on + strand at 101.652 kb on + strand at 101.653 kb on - strand at 101.700 kb on + strand, within Echvi_0104 at 101.701 kb on - strand, within Echvi_0104 at 101.739 kb on - strand, within Echvi_0104 at 101.973 kb on + strand at 101.973 kb on + strand at 101.973 kb on + strand at 101.974 kb on - strand at 102.152 kb on + strand, within Echvi_0105 at 102.153 kb on - strand, within Echvi_0105 at 102.153 kb on - strand, within Echvi_0105 at 102.229 kb on + strand, within Echvi_0105 at 102.229 kb on + strand, within Echvi_0105 at 102.230 kb on - strand, within Echvi_0105 at 102.230 kb on - strand, within Echvi_0105 at 102.231 kb on + strand, within Echvi_0105 at 102.231 kb on + strand, within Echvi_0105 at 102.232 kb on - strand, within Echvi_0105 at 102.267 kb on - strand, within Echvi_0105 at 102.361 kb on + strand, within Echvi_0105 at 102.362 kb on - strand, within Echvi_0105 at 102.455 kb on + strand at 102.455 kb on + strand at 102.456 kb on - strand at 102.456 kb on - strand at 102.458 kb on - strand at 102.476 kb on - strand at 102.501 kb on - strand at 102.522 kb on + strand at 102.524 kb on + strand at 102.601 kb on + strand, within Echvi_0106 at 102.688 kb on - strand, within Echvi_0106 at 102.688 kb on - strand, within Echvi_0106 at 102.688 kb on - strand, within Echvi_0106 at 102.691 kb on + strand, within Echvi_0106 at 102.691 kb on + strand, within Echvi_0106 at 102.692 kb on - strand, within Echvi_0106 at 102.919 kb on + strand, within Echvi_0106 at 102.920 kb on - strand, within Echvi_0106 at 102.920 kb on - strand, within Echvi_0106 at 102.920 kb on - strand, within Echvi_0106 at 102.921 kb on + strand, within Echvi_0106 at 102.921 kb on + strand, within Echvi_0106 at 102.952 kb on + strand at 102.953 kb on - strand at 102.961 kb on + strand at 102.984 kb on + strand at 102.984 kb on + strand at 102.984 kb on + strand at 102.985 kb on - strand at 103.357 kb on - strand at 103.491 kb on - strand, within Echvi_0108 at 103.684 kb on - strand, within Echvi_0108 at 103.833 kb on + strand, within Echvi_0108 at 103.834 kb on - strand, within Echvi_0108
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.12 mg/ml remove 101,536 - +0.8 101,538 + +0.4 101,559 + -1.0 101,587 + +0.2 101,588 - +0.2 101,629 + +0.4 101,652 + +1.3 101,652 + -1.2 101,653 - -0.2 101,700 + Echvi_0104 0.19 +0.4 101,701 - Echvi_0104 0.20 -0.8 101,739 - Echvi_0104 0.31 +0.3 101,973 + +0.0 101,973 + -0.5 101,973 + +0.3 101,974 - -0.7 102,152 + Echvi_0105 0.35 +1.3 102,153 - Echvi_0105 0.35 -1.2 102,153 - Echvi_0105 0.35 -0.4 102,229 + Echvi_0105 0.50 -0.4 102,229 + Echvi_0105 0.50 -0.3 102,230 - Echvi_0105 0.50 +1.2 102,230 - Echvi_0105 0.50 -0.1 102,231 + Echvi_0105 0.51 +0.2 102,231 + Echvi_0105 0.51 +0.2 102,232 - Echvi_0105 0.51 +0.0 102,267 - Echvi_0105 0.58 -0.0 102,361 + Echvi_0105 0.77 -1.6 102,362 - Echvi_0105 0.77 +0.0 102,455 + -1.6 102,455 + -0.3 102,456 - -0.2 102,456 - -0.1 102,458 - +0.1 102,476 - -0.3 102,501 - -0.6 102,522 + +0.0 102,524 + -0.0 102,601 + Echvi_0106 0.17 -1.9 102,688 - Echvi_0106 0.36 -1.3 102,688 - Echvi_0106 0.36 +0.2 102,688 - Echvi_0106 0.36 -0.5 102,691 + Echvi_0106 0.37 -1.0 102,691 + Echvi_0106 0.37 -0.1 102,692 - Echvi_0106 0.37 -2.2 102,919 + Echvi_0106 0.86 -0.7 102,920 - Echvi_0106 0.86 -0.4 102,920 - Echvi_0106 0.86 +1.8 102,920 - Echvi_0106 0.86 +0.9 102,921 + Echvi_0106 0.87 +0.2 102,921 + Echvi_0106 0.87 -0.6 102,952 + -0.5 102,953 - +2.8 102,961 + -0.6 102,984 + -0.4 102,984 + +0.4 102,984 + +0.8 102,985 - -0.2 103,357 - +1.1 103,491 - Echvi_0108 0.15 -2.2 103,684 - Echvi_0108 0.37 +0.4 103,833 + Echvi_0108 0.54 -0.8 103,834 - Echvi_0108 0.54 +0.2
Or see this region's nucleotide sequence