Experiment: m.b. Spectinomycin 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0023 and Echvi_0024 are separated by 63 nucleotides Echvi_0024 and Echvi_0025 are separated by 6 nucleotides Echvi_0025 and Echvi_0026 are separated by 159 nucleotides
Echvi_0023: Echvi_0023 - phenylalanyl-tRNA synthetase, beta subunit, non-spirochete bacterial, at 21,000 to 23,411
_0023
Echvi_0024: Echvi_0024 - Protein of unknown function (DUF1684)., at 23,475 to 24,092
_0024
Echvi_0025: Echvi_0025 - DNA repair proteins, at 24,099 to 24,284
_0025
Echvi_0026: Echvi_0026 - Site-specific recombinase XerD, at 24,444 to 25,712
_0026
Position (kb) 23
24
25 Strain fitness (log2 ratio) -2
-1
0
1
2 at 23.563 kb on + strand, within Echvi_0024 at 23.581 kb on + strand, within Echvi_0024 at 23.638 kb on + strand, within Echvi_0024 at 23.653 kb on + strand, within Echvi_0024 at 23.653 kb on + strand, within Echvi_0024 at 23.773 kb on + strand, within Echvi_0024 at 23.773 kb on + strand, within Echvi_0024 at 23.774 kb on - strand, within Echvi_0024 at 23.774 kb on - strand, within Echvi_0024 at 23.895 kb on + strand, within Echvi_0024 at 23.948 kb on - strand, within Echvi_0024 at 24.063 kb on + strand at 24.075 kb on + strand at 24.151 kb on - strand, within Echvi_0025 at 24.159 kb on + strand, within Echvi_0025 at 24.240 kb on - strand, within Echvi_0025 at 24.263 kb on + strand, within Echvi_0025 at 24.268 kb on - strand at 24.268 kb on - strand at 24.268 kb on - strand at 24.279 kb on + strand at 24.279 kb on + strand at 24.280 kb on - strand at 24.280 kb on - strand at 24.311 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.12 mg/ml remove 23,563 + Echvi_0024 0.14 -0.5 23,581 + Echvi_0024 0.17 -2.6 23,638 + Echvi_0024 0.26 -2.2 23,653 + Echvi_0024 0.29 -0.6 23,653 + Echvi_0024 0.29 -0.5 23,773 + Echvi_0024 0.48 -0.9 23,773 + Echvi_0024 0.48 -2.2 23,774 - Echvi_0024 0.48 +0.0 23,774 - Echvi_0024 0.48 -2.1 23,895 + Echvi_0024 0.68 -0.6 23,948 - Echvi_0024 0.77 -1.5 24,063 + -0.7 24,075 + +0.0 24,151 - Echvi_0025 0.28 +0.0 24,159 + Echvi_0025 0.32 -0.8 24,240 - Echvi_0025 0.76 -0.1 24,263 + Echvi_0025 0.88 +0.8 24,268 - +1.8 24,268 - +1.2 24,268 - -0.3 24,279 + -0.0 24,279 + -0.7 24,280 - -0.2 24,280 - -0.5 24,311 - +1.8
Or see this region's nucleotide sequence