Experiment: m.b. Spectinomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0437 and Echvi_0438 are separated by 33 nucleotides Echvi_0438 and Echvi_0439 overlap by 61 nucleotides Echvi_0439 and Echvi_0440 are separated by 212 nucleotides Echvi_0440 and Echvi_0441 are separated by 785 nucleotides
Echvi_0437: Echvi_0437 - DNA primase (bacterial type), at 477,193 to 478,056
_0437
Echvi_0438: Echvi_0438 - hypothetical protein, at 478,090 to 478,263
_0438
Echvi_0439: Echvi_0439 - hypothetical protein, at 478,203 to 478,484
_0439
Echvi_0440: Echvi_0440 - DNA repair proteins, at 478,697 to 479,167
_0440
Echvi_0441: Echvi_0441 - Predicted ATP-dependent endonuclease of the OLD family, at 479,953 to 481,680
_0441
Position (kb) 478
479
480 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 477.727 kb on - strand, within Echvi_0437 at 477.727 kb on - strand, within Echvi_0437 at 477.806 kb on + strand, within Echvi_0437 at 477.806 kb on + strand, within Echvi_0437 at 477.876 kb on - strand, within Echvi_0437 at 477.901 kb on + strand, within Echvi_0437 at 477.901 kb on + strand, within Echvi_0437 at 478.059 kb on + strand at 478.060 kb on - strand at 478.095 kb on + strand at 478.096 kb on - strand at 478.099 kb on - strand at 478.132 kb on - strand, within Echvi_0438 at 478.163 kb on + strand, within Echvi_0438 at 478.164 kb on - strand, within Echvi_0438 at 478.172 kb on + strand, within Echvi_0438 at 478.172 kb on + strand, within Echvi_0438 at 478.523 kb on + strand at 478.523 kb on + strand at 478.573 kb on + strand at 478.648 kb on + strand at 478.648 kb on + strand at 478.720 kb on - strand at 478.751 kb on - strand, within Echvi_0440 at 478.784 kb on + strand, within Echvi_0440 at 478.784 kb on + strand, within Echvi_0440 at 479.447 kb on - strand at 479.475 kb on + strand at 479.475 kb on + strand at 479.475 kb on + strand at 479.509 kb on + strand at 479.509 kb on + strand at 479.515 kb on + strand at 479.515 kb on + strand at 479.575 kb on + strand at 479.576 kb on - strand at 479.587 kb on + strand at 479.588 kb on - strand at 479.599 kb on - strand at 479.622 kb on + strand at 479.624 kb on + strand at 479.625 kb on - strand at 479.647 kb on - strand at 479.655 kb on + strand at 479.691 kb on + strand at 479.703 kb on + strand at 479.719 kb on + strand at 479.735 kb on - strand at 479.735 kb on - strand at 479.736 kb on + strand at 479.736 kb on + strand at 479.737 kb on - strand at 479.742 kb on + strand at 479.743 kb on - strand at 479.816 kb on + strand at 479.817 kb on - strand at 479.936 kb on + strand at 479.936 kb on + strand at 479.937 kb on - strand at 479.937 kb on - strand at 479.937 kb on - strand at 479.938 kb on + strand at 479.938 kb on + strand at 479.938 kb on + strand at 479.938 kb on + strand at 479.938 kb on + strand at 479.939 kb on - strand at 479.939 kb on - strand at 479.939 kb on - strand at 480.026 kb on + strand at 480.026 kb on + strand at 480.026 kb on + strand at 480.026 kb on + strand at 480.026 kb on + strand at 480.027 kb on - strand at 480.030 kb on - strand at 480.035 kb on + strand at 480.035 kb on + strand at 480.042 kb on + strand at 480.045 kb on + strand at 480.046 kb on - strand at 480.046 kb on - strand at 480.046 kb on - strand at 480.049 kb on - strand at 480.065 kb on + strand at 480.065 kb on + strand at 480.089 kb on + strand at 480.099 kb on + strand at 480.100 kb on - strand at 480.100 kb on - strand at 480.140 kb on - strand, within Echvi_0441 at 480.150 kb on - strand, within Echvi_0441 at 480.151 kb on + strand, within Echvi_0441 at 480.156 kb on + strand, within Echvi_0441 at 480.157 kb on - strand, within Echvi_0441
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.06 mg/ml remove 477,727 - Echvi_0437 0.62 +0.9 477,727 - Echvi_0437 0.62 -0.2 477,806 + Echvi_0437 0.71 -0.9 477,806 + Echvi_0437 0.71 +0.6 477,876 - Echvi_0437 0.79 +0.1 477,901 + Echvi_0437 0.82 -0.2 477,901 + Echvi_0437 0.82 -0.6 478,059 + -0.2 478,060 - -0.4 478,095 + +0.2 478,096 - -0.1 478,099 - -0.2 478,132 - Echvi_0438 0.24 -0.4 478,163 + Echvi_0438 0.42 -0.3 478,164 - Echvi_0438 0.43 -0.1 478,172 + Echvi_0438 0.47 +0.5 478,172 + Echvi_0438 0.47 -0.8 478,523 + +1.1 478,523 + -0.2 478,573 + -0.4 478,648 + -0.5 478,648 + -0.7 478,720 - +0.5 478,751 - Echvi_0440 0.11 -1.5 478,784 + Echvi_0440 0.18 -0.4 478,784 + Echvi_0440 0.18 +0.0 479,447 - +0.3 479,475 + -0.1 479,475 + -0.8 479,475 + +0.9 479,509 + -1.1 479,509 + -0.2 479,515 + -1.6 479,515 + +0.3 479,575 + +0.3 479,576 - -0.4 479,587 + +0.2 479,588 - -0.5 479,599 - +0.7 479,622 + -1.4 479,624 + -0.2 479,625 - +0.3 479,647 - -0.4 479,655 + -0.7 479,691 + -0.3 479,703 + -0.2 479,719 + -0.9 479,735 - -0.6 479,735 - -0.0 479,736 + +3.8 479,736 + +1.7 479,737 - +0.6 479,742 + +0.1 479,743 - +1.5 479,816 + -0.6 479,817 - -0.0 479,936 + -1.4 479,936 + -0.1 479,937 - +0.5 479,937 - -1.4 479,937 - -0.1 479,938 + -0.9 479,938 + -0.5 479,938 + -0.2 479,938 + -0.1 479,938 + -2.0 479,939 - +0.1 479,939 - -0.9 479,939 - +0.0 480,026 + +0.0 480,026 + -1.0 480,026 + +1.2 480,026 + -0.6 480,026 + +0.5 480,027 - -0.3 480,030 - +1.7 480,035 + -0.2 480,035 + -0.1 480,042 + -0.2 480,045 + +0.2 480,046 - -0.2 480,046 - +0.0 480,046 - -0.1 480,049 - -0.1 480,065 + +0.9 480,065 + -0.2 480,089 + -0.3 480,099 + +0.7 480,100 - -0.5 480,100 - -0.9 480,140 - Echvi_0441 0.11 +0.0 480,150 - Echvi_0441 0.11 -0.5 480,151 + Echvi_0441 0.11 -0.5 480,156 + Echvi_0441 0.12 -1.1 480,157 - Echvi_0441 0.12 -1.1
Or see this region's nucleotide sequence