Experiment: copper (II) chloride 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisA and hisF overlap by 19 nucleotides hisF and hisI overlap by 7 nucleotides hisI and wzzB are separated by 95 nucleotides
b2024: hisA - N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS), at 2,093,149 to 2,093,886
hisA
b2025: hisF - imidazole glycerol phosphate synthase subunit HisF (NCBI), at 2,093,868 to 2,094,644
hisF
b2026: hisI - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein (NCBI), at 2,094,638 to 2,095,249
hisI
b2027: wzzB - regulator of length of O-antigen component of lipopolysaccharide chains (VIMSS), at 2,095,345 to 2,096,325
wzzB
Position (kb)
2094
2095
2096 Strain fitness (log2 ratio)
-2
-1
0
1 at 2093.818 kb on - strand at 2093.818 kb on - strand at 2093.881 kb on + strand at 2093.918 kb on + strand at 2093.978 kb on + strand, within hisF at 2094.236 kb on + strand, within hisF at 2094.236 kb on + strand, within hisF at 2094.236 kb on + strand, within hisF at 2094.244 kb on - strand, within hisF at 2094.244 kb on - strand, within hisF at 2094.248 kb on - strand, within hisF at 2094.338 kb on - strand, within hisF at 2094.596 kb on + strand at 2094.596 kb on + strand at 2094.647 kb on + strand at 2094.712 kb on - strand, within hisI at 2094.712 kb on - strand, within hisI at 2094.716 kb on + strand, within hisI at 2094.869 kb on + strand, within hisI at 2094.907 kb on - strand, within hisI at 2094.907 kb on - strand, within hisI at 2095.032 kb on + strand, within hisI at 2095.033 kb on + strand, within hisI at 2095.033 kb on + strand, within hisI at 2095.124 kb on + strand, within hisI at 2095.167 kb on + strand, within hisI at 2095.202 kb on - strand at 2095.231 kb on + strand at 2095.386 kb on - strand at 2095.490 kb on + strand, within wzzB at 2095.494 kb on + strand, within wzzB at 2095.586 kb on - strand, within wzzB at 2095.586 kb on - strand, within wzzB at 2095.606 kb on - strand, within wzzB at 2095.719 kb on - strand, within wzzB at 2095.719 kb on - strand, within wzzB at 2095.729 kb on - strand, within wzzB at 2095.819 kb on + strand, within wzzB at 2095.819 kb on + strand, within wzzB at 2095.900 kb on + strand, within wzzB at 2095.900 kb on + strand, within wzzB at 2096.075 kb on + strand, within wzzB at 2096.075 kb on + strand, within wzzB at 2096.102 kb on + strand, within wzzB at 2096.163 kb on - strand, within wzzB
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 2 mM remove 2,093,818 - -0.2 2,093,818 - -0.1 2,093,881 + -0.1 2,093,918 + -1.0 2,093,978 + hisF b2025 0.14 -1.1 2,094,236 + hisF b2025 0.47 +0.0 2,094,236 + hisF b2025 0.47 -1.0 2,094,236 + hisF b2025 0.47 -1.4 2,094,244 - hisF b2025 0.48 -0.5 2,094,244 - hisF b2025 0.48 -1.2 2,094,248 - hisF b2025 0.49 -2.7 2,094,338 - hisF b2025 0.60 -0.3 2,094,596 + -0.2 2,094,596 + -0.4 2,094,647 + +0.2 2,094,712 - hisI b2026 0.12 -0.5 2,094,712 - hisI b2026 0.12 -2.5 2,094,716 + hisI b2026 0.13 +0.3 2,094,869 + hisI b2026 0.38 +0.5 2,094,907 - hisI b2026 0.44 -1.4 2,094,907 - hisI b2026 0.44 -1.5 2,095,032 + hisI b2026 0.64 -0.9 2,095,033 + hisI b2026 0.65 -1.0 2,095,033 + hisI b2026 0.65 -0.7 2,095,124 + hisI b2026 0.79 -0.5 2,095,167 + hisI b2026 0.86 -0.3 2,095,202 - +0.5 2,095,231 + +0.2 2,095,386 - -0.5 2,095,490 + wzzB b2027 0.15 -0.5 2,095,494 + wzzB b2027 0.15 -0.1 2,095,586 - wzzB b2027 0.25 -0.3 2,095,586 - wzzB b2027 0.25 +1.3 2,095,606 - wzzB b2027 0.27 -0.8 2,095,719 - wzzB b2027 0.38 -0.1 2,095,719 - wzzB b2027 0.38 +0.3 2,095,729 - wzzB b2027 0.39 +1.0 2,095,819 + wzzB b2027 0.48 -0.1 2,095,819 + wzzB b2027 0.48 -0.1 2,095,900 + wzzB b2027 0.57 -0.3 2,095,900 + wzzB b2027 0.57 +0.9 2,096,075 + wzzB b2027 0.74 -0.5 2,096,075 + wzzB b2027 0.74 -0.9 2,096,102 + wzzB b2027 0.77 +0.5 2,096,163 - wzzB b2027 0.83 +0.5
Or see this region's nucleotide sequence