Experiment: copper (II) chloride 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt manX and manY are separated by 62 nucleotides manY and manZ are separated by 12 nucleotides manZ and yobD are separated by 54 nucleotides
b1817: manX - fused mannose-specific PTS enzymes: IIA component/IIB component (NCBI), at 1,900,072 to 1,901,043
manX
b1818: manY - mannose-specific enzyme IIC component of PTS (NCBI), at 1,901,106 to 1,901,906
manY
b1819: manZ - mannose-specific enzyme IID component of PTS (NCBI), at 1,901,919 to 1,902,770
manZ
b1820: yobD - hypothetical protein (NCBI), at 1,902,825 to 1,903,283
yobD
Position (kb)
1901
1902 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1900.112 kb on - strand at 1900.117 kb on - strand at 1900.225 kb on + strand, within manX at 1900.253 kb on - strand, within manX at 1900.413 kb on + strand, within manX at 1900.611 kb on + strand, within manX at 1900.651 kb on - strand, within manX at 1900.702 kb on + strand, within manX at 1900.730 kb on + strand, within manX at 1900.787 kb on + strand, within manX at 1900.864 kb on - strand, within manX at 1900.885 kb on - strand, within manX at 1900.885 kb on - strand, within manX at 1900.887 kb on + strand, within manX at 1900.887 kb on + strand, within manX at 1900.964 kb on + strand at 1900.972 kb on - strand at 1901.109 kb on - strand at 1901.109 kb on - strand at 1901.147 kb on + strand at 1901.150 kb on - strand at 1901.150 kb on - strand at 1901.406 kb on + strand, within manY at 1901.560 kb on - strand, within manY at 1901.794 kb on - strand, within manY at 1902.131 kb on + strand, within manZ at 1902.131 kb on + strand, within manZ at 1902.139 kb on - strand, within manZ at 1902.139 kb on - strand, within manZ at 1902.146 kb on + strand, within manZ at 1902.425 kb on - strand, within manZ at 1902.428 kb on + strand, within manZ at 1902.618 kb on + strand, within manZ at 1902.618 kb on + strand, within manZ at 1902.626 kb on - strand, within manZ at 1902.758 kb on + strand at 1902.763 kb on + strand at 1902.765 kb on + strand at 1902.765 kb on - strand at 1902.766 kb on - strand at 1902.773 kb on - strand at 1902.781 kb on - strand at 1902.867 kb on + strand at 1902.867 kb on + strand at 1902.875 kb on - strand, within yobD at 1902.875 kb on - strand at 1902.875 kb on - strand, within yobD at 1902.882 kb on + strand, within yobD at 1902.893 kb on + strand, within yobD
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 2 mM remove 1,900,112 - +0.3 1,900,117 - +0.8 1,900,225 + manX b1817 0.16 +0.3 1,900,253 - manX b1817 0.19 -0.4 1,900,413 + manX b1817 0.35 +1.1 1,900,611 + manX b1817 0.55 +0.5 1,900,651 - manX b1817 0.60 -0.6 1,900,702 + manX b1817 0.65 -0.0 1,900,730 + manX b1817 0.68 -0.9 1,900,787 + manX b1817 0.74 -0.1 1,900,864 - manX b1817 0.81 +0.6 1,900,885 - manX b1817 0.84 -1.7 1,900,885 - manX b1817 0.84 -0.7 1,900,887 + manX b1817 0.84 +0.7 1,900,887 + manX b1817 0.84 +0.7 1,900,964 + +0.9 1,900,972 - -0.7 1,901,109 - +0.0 1,901,109 - +0.5 1,901,147 + -2.0 1,901,150 - +0.5 1,901,150 - +0.3 1,901,406 + manY b1818 0.37 -2.5 1,901,560 - manY b1818 0.57 -0.7 1,901,794 - manY b1818 0.86 -0.3 1,902,131 + manZ b1819 0.25 -1.2 1,902,131 + manZ b1819 0.25 +1.7 1,902,139 - manZ b1819 0.26 -0.1 1,902,139 - manZ b1819 0.26 +1.6 1,902,146 + manZ b1819 0.27 -0.9 1,902,425 - manZ b1819 0.59 -2.8 1,902,428 + manZ b1819 0.60 -0.1 1,902,618 + manZ b1819 0.82 -0.7 1,902,618 + manZ b1819 0.82 +1.1 1,902,626 - manZ b1819 0.83 +0.9 1,902,758 + -0.0 1,902,763 + -1.5 1,902,765 + -0.0 1,902,765 - +0.7 1,902,766 - +0.3 1,902,773 - +0.0 1,902,781 - -0.2 1,902,867 + +1.2 1,902,867 + -0.7 1,902,875 - yobD b1820 0.11 +0.9 1,902,875 - -1.2 1,902,875 - yobD b1820 0.11 -0.5 1,902,882 + yobD b1820 0.12 +0.3 1,902,893 + yobD b1820 0.15 -0.3
Or see this region's nucleotide sequence