Experiment: copper (II) chloride 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt citF and citE are separated by 10 nucleotides citE and citD overlap by 4 nucleotides citD and citC are separated by 14 nucleotides
b0615: citF - citrate lyase, citrate-ACP transferase (alpha) subunit (NCBI), at 647,262 to 648,794
citF
b0616: citE - citrate lyase beta chain (acyl lyase subunit) (VIMSS), at 648,805 to 649,713
citE
b0617: citD - citrate lyase, acyl carrier (gamma) subunit (NCBI), at 649,710 to 650,006
citD
b0618: citC - citrate lyase synthetase (citrate (pro-3S)-lyase ligase (VIMSS), at 650,021 to 651,079
citC
Position (kb) 649
650
651 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 648.810 kb on - strand at 649.122 kb on - strand, within citE at 649.238 kb on - strand, within citE at 649.238 kb on - strand, within citE at 649.327 kb on - strand, within citE at 649.479 kb on + strand, within citE at 649.540 kb on - strand, within citE at 649.569 kb on + strand, within citE at 649.595 kb on - strand, within citE at 649.595 kb on - strand, within citE at 649.607 kb on + strand, within citE at 649.642 kb on + strand at 649.665 kb on - strand at 649.828 kb on - strand, within citD at 649.828 kb on - strand, within citD at 649.831 kb on - strand, within citD at 649.947 kb on - strand, within citD at 650.199 kb on + strand, within citC at 650.221 kb on + strand, within citC at 650.310 kb on - strand, within citC at 650.326 kb on + strand, within citC at 650.332 kb on - strand, within citC at 650.332 kb on - strand at 650.456 kb on - strand, within citC at 650.577 kb on + strand, within citC at 650.733 kb on - strand, within citC at 650.794 kb on + strand, within citC at 650.808 kb on + strand, within citC at 650.808 kb on + strand, within citC at 650.808 kb on + strand, within citC at 650.816 kb on - strand, within citC at 650.818 kb on + strand, within citC at 650.818 kb on + strand, within citC at 650.818 kb on + strand, within citC at 650.858 kb on + strand, within citC at 650.860 kb on - strand, within citC at 650.863 kb on + strand, within citC at 650.863 kb on + strand, within citC at 650.863 kb on + strand, within citC at 650.885 kb on + strand, within citC at 650.885 kb on + strand, within citC at 650.885 kb on + strand, within citC at 650.888 kb on + strand, within citC at 650.888 kb on + strand, within citC at 650.888 kb on - strand, within citC at 650.955 kb on + strand, within citC at 650.955 kb on + strand, within citC at 650.962 kb on + strand, within citC at 650.966 kb on + strand, within citC at 650.966 kb on + strand, within citC at 650.978 kb on + strand at 650.978 kb on + strand at 650.988 kb on + strand at 650.988 kb on + strand at 650.992 kb on + strand at 650.992 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 2 mM remove 648,810 - +0.7 649,122 - citE b0616 0.35 -0.4 649,238 - citE b0616 0.48 +2.1 649,238 - citE b0616 0.48 +0.7 649,327 - citE b0616 0.57 +1.0 649,479 + citE b0616 0.74 +0.4 649,540 - citE b0616 0.81 +0.7 649,569 + citE b0616 0.84 +0.5 649,595 - citE b0616 0.87 +0.2 649,595 - citE b0616 0.87 -2.8 649,607 + citE b0616 0.88 +0.6 649,642 + +0.5 649,665 - -0.1 649,828 - citD b0617 0.40 -0.3 649,828 - citD b0617 0.40 -1.2 649,831 - citD b0617 0.41 +0.2 649,947 - citD b0617 0.80 -0.3 650,199 + citC b0618 0.17 -0.8 650,221 + citC b0618 0.19 +0.5 650,310 - citC b0618 0.27 -0.4 650,326 + citC b0618 0.29 +0.3 650,332 - citC b0618 0.29 +0.7 650,332 - +1.3 650,456 - citC b0618 0.41 -0.4 650,577 + citC b0618 0.53 +0.5 650,733 - citC b0618 0.67 +0.2 650,794 + citC b0618 0.73 -1.5 650,808 + citC b0618 0.74 -0.1 650,808 + citC b0618 0.74 -0.3 650,808 + citC b0618 0.74 +1.3 650,816 - citC b0618 0.75 +0.3 650,818 + citC b0618 0.75 +0.7 650,818 + citC b0618 0.75 +0.7 650,818 + citC b0618 0.75 +0.2 650,858 + citC b0618 0.79 -0.5 650,860 - citC b0618 0.79 -0.1 650,863 + citC b0618 0.80 -0.1 650,863 + citC b0618 0.80 +0.4 650,863 + citC b0618 0.80 +1.5 650,885 + citC b0618 0.82 -0.3 650,885 + citC b0618 0.82 +0.2 650,885 + citC b0618 0.82 +1.0 650,888 + citC b0618 0.82 +1.2 650,888 + citC b0618 0.82 +0.4 650,888 - citC b0618 0.82 +0.2 650,955 + citC b0618 0.88 +0.5 650,955 + citC b0618 0.88 +0.2 650,962 + citC b0618 0.89 +0.1 650,966 + citC b0618 0.89 -0.8 650,966 + citC b0618 0.89 -0.3 650,978 + -1.0 650,978 + +0.5 650,988 + -0.3 650,988 + -0.8 650,992 + -0.5 650,992 + +1.0
Or see this region's nucleotide sequence