Strain Fitness in Ralstonia solanacearum UW163 around UW163_RS12300

Experiment: M63_quarter_strength

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntUW163_RS12295 and UW163_RS12300 overlap by 4 nucleotidesUW163_RS12300 and UW163_RS12305 are separated by 115 nucleotides UW163_RS12295: UW163_RS12295 - molecular chaperone Hsp33, at 2,637,311 to 2,638,327 _RS12295 UW163_RS12300: UW163_RS12300 - gamma carbonic anhydrase family protein, at 2,638,324 to 2,638,851 _RS12300 UW163_RS12305: UW163_RS12305 - DUF455 domain-containing protein, at 2,638,967 to 2,639,797 _RS12305 Position (kb) 2638 2639Strain fitness (log2 ratio) -2 -1 0 1 2at 2637.771 kb on + strand, within UW163_RS12295at 2637.772 kb on - strand, within UW163_RS12295at 2637.865 kb on - strand, within UW163_RS12295at 2637.865 kb on - strand, within UW163_RS12295at 2638.355 kb on + strandat 2638.355 kb on + strandat 2638.356 kb on - strandat 2638.376 kb on + strandat 2638.377 kb on - strand, within UW163_RS12300at 2638.657 kb on - strand, within UW163_RS12300at 2638.799 kb on + strandat 2638.875 kb on + strandat 2639.322 kb on - strand, within UW163_RS12305at 2639.699 kb on + strand, within UW163_RS12305at 2639.795 kb on + strandat 2639.795 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M63_quarter_strength
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2,637,771 + UW163_RS12295 0.45 -1.0
2,637,772 - UW163_RS12295 0.45 +0.3
2,637,865 - UW163_RS12295 0.54 +0.2
2,637,865 - UW163_RS12295 0.54 +0.8
2,638,355 + +0.2
2,638,355 + +0.4
2,638,356 - +0.5
2,638,376 + +0.6
2,638,377 - UW163_RS12300 0.10 +0.5
2,638,657 - UW163_RS12300 0.63 -2.5
2,638,799 + +1.4
2,638,875 + -0.5
2,639,322 - UW163_RS12305 0.43 +0.1
2,639,699 + UW163_RS12305 0.88 +0.4
2,639,795 + -0.5
2,639,795 + +2.3

Or see this region's nucleotide sequence