Experiment: M63_quarter_strength
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt UW163_RS10390 and UW163_RS10395 are separated by 17 nucleotides UW163_RS10395 and UW163_RS10400 are separated by 131 nucleotides UW163_RS10400 and UW163_RS10405 are separated by 80 nucleotides UW163_RS10405 and UW163_RS10410 overlap by 38 nucleotides
UW163_RS10390: UW163_RS10390 - YicC family protein, at 2,256,082 to 2,257,017
_RS10390
UW163_RS10395: UW163_RS10395 - guanylate kinase, at 2,257,035 to 2,257,700
_RS10395
UW163_RS10400: UW163_RS10400 - DNA-directed RNA polymerase subunit omega, at 2,257,832 to 2,258,035
_RS10400
UW163_RS10405: UW163_RS10405 - hypothetical protein, at 2,258,116 to 2,258,307
_RS10405
UW163_RS10410: UW163_RS10410 - bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, at 2,258,270 to 2,260,696
_RS10410
Position (kb)
2257
2258
2259 Strain fitness (log2 ratio)
-1
0
1 at 2257.644 kb on + strand at 2257.644 kb on + strand at 2257.644 kb on + strand at 2257.645 kb on - strand at 2257.645 kb on - strand at 2257.646 kb on + strand at 2257.741 kb on + strand at 2257.742 kb on - strand at 2257.789 kb on + strand at 2257.840 kb on + strand at 2257.904 kb on + strand, within UW163_RS10400 at 2258.044 kb on + strand at 2258.059 kb on + strand at 2258.059 kb on + strand at 2258.137 kb on + strand, within UW163_RS10405 at 2258.409 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M63_quarter_strength remove 2,257,644 + +0.6 2,257,644 + +0.7 2,257,644 + +0.7 2,257,645 - +0.1 2,257,645 - -0.3 2,257,646 + -0.2 2,257,741 + +0.2 2,257,742 - -0.1 2,257,789 + +0.7 2,257,840 + -1.3 2,257,904 + UW163_RS10400 0.35 -0.7 2,258,044 + -0.4 2,258,059 + +0.2 2,258,059 + +0.4 2,258,137 + UW163_RS10405 0.11 -0.2 2,258,409 + -0.0
Or see this region's nucleotide sequence