Strain Fitness in Ralstonia solanacearum UW163 around UW163_RS10400

Experiment: M63_quarter_strength

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntUW163_RS10390 and UW163_RS10395 are separated by 17 nucleotidesUW163_RS10395 and UW163_RS10400 are separated by 131 nucleotidesUW163_RS10400 and UW163_RS10405 are separated by 80 nucleotidesUW163_RS10405 and UW163_RS10410 overlap by 38 nucleotides UW163_RS10390: UW163_RS10390 - YicC family protein, at 2,256,082 to 2,257,017 _RS10390 UW163_RS10395: UW163_RS10395 - guanylate kinase, at 2,257,035 to 2,257,700 _RS10395 UW163_RS10400: UW163_RS10400 - DNA-directed RNA polymerase subunit omega, at 2,257,832 to 2,258,035 _RS10400 UW163_RS10405: UW163_RS10405 - hypothetical protein, at 2,258,116 to 2,258,307 _RS10405 UW163_RS10410: UW163_RS10410 - bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, at 2,258,270 to 2,260,696 _RS10410 Position (kb) 2257 2258 2259Strain fitness (log2 ratio) -1 0 1at 2257.644 kb on + strandat 2257.644 kb on + strandat 2257.644 kb on + strandat 2257.645 kb on - strandat 2257.645 kb on - strandat 2257.646 kb on + strandat 2257.741 kb on + strandat 2257.742 kb on - strandat 2257.789 kb on + strandat 2257.840 kb on + strandat 2257.904 kb on + strand, within UW163_RS10400at 2258.044 kb on + strandat 2258.059 kb on + strandat 2258.059 kb on + strandat 2258.137 kb on + strand, within UW163_RS10405at 2258.409 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M63_quarter_strength
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2,257,644 + +0.6
2,257,644 + +0.7
2,257,644 + +0.7
2,257,645 - +0.1
2,257,645 - -0.3
2,257,646 + -0.2
2,257,741 + +0.2
2,257,742 - -0.1
2,257,789 + +0.7
2,257,840 + -1.3
2,257,904 + UW163_RS10400 0.35 -0.7
2,258,044 + -0.4
2,258,059 + +0.2
2,258,059 + +0.4
2,258,137 + UW163_RS10405 0.11 -0.2
2,258,409 + -0.0

Or see this region's nucleotide sequence