Experiment: Citric Acid (C) 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFR28_00682 and PFR28_00683 are separated by 232 nucleotides PFR28_00683 and PFR28_00684 overlap by 8 nucleotides PFR28_00684 and PFR28_00685 are separated by 85 nucleotides PFR28_00685 and PFR28_00686 are separated by 35 nucleotides
PFR28_00682: PFR28_00682 - hypothetical protein, at 725,942 to 726,127
_00682
PFR28_00683: PFR28_00683 - Transcriptional regulatory protein tctD, at 726,360 to 727,031
_00683
PFR28_00684: PFR28_00684 - Sensor protein QseC, at 727,024 to 728,415
_00684
PFR28_00685: PFR28_00685 - hypothetical protein, at 728,501 to 729,322
_00685
PFR28_00686: PFR28_00686 - tRNA-modifying protein YgfZ, at 729,358 to 730,299
_00686
Position (kb)
727
728
729 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 726.024 kb on + strand, within PFR28_00682 at 726.024 kb on + strand, within PFR28_00682 at 726.024 kb on + strand, within PFR28_00682 at 726.025 kb on - strand, within PFR28_00682 at 726.025 kb on - strand, within PFR28_00682 at 726.025 kb on - strand, within PFR28_00682 at 726.025 kb on - strand, within PFR28_00682 at 726.134 kb on + strand at 726.134 kb on + strand at 726.134 kb on + strand at 726.134 kb on + strand at 726.135 kb on - strand at 726.135 kb on - strand at 726.144 kb on + strand at 726.145 kb on - strand at 726.145 kb on - strand at 726.145 kb on - strand at 726.153 kb on + strand at 726.217 kb on - strand at 726.217 kb on - strand at 726.232 kb on + strand at 726.232 kb on + strand at 726.252 kb on - strand at 726.355 kb on + strand at 726.355 kb on + strand at 726.355 kb on + strand at 726.356 kb on - strand at 726.356 kb on - strand at 726.356 kb on - strand at 726.356 kb on - strand at 726.492 kb on + strand, within PFR28_00683 at 726.493 kb on - strand, within PFR28_00683 at 726.514 kb on + strand, within PFR28_00683 at 726.515 kb on - strand, within PFR28_00683 at 726.515 kb on - strand, within PFR28_00683 at 726.760 kb on - strand, within PFR28_00683 at 726.760 kb on - strand, within PFR28_00683 at 726.760 kb on - strand, within PFR28_00683 at 726.760 kb on - strand, within PFR28_00683 at 726.760 kb on - strand, within PFR28_00683 at 726.760 kb on - strand, within PFR28_00683 at 726.937 kb on - strand, within PFR28_00683 at 726.937 kb on - strand, within PFR28_00683 at 726.937 kb on - strand, within PFR28_00683 at 727.006 kb on - strand at 727.029 kb on + strand at 727.029 kb on + strand at 727.030 kb on - strand at 727.030 kb on - strand at 727.030 kb on - strand at 727.030 kb on - strand at 727.030 kb on - strand at 727.214 kb on + strand, within PFR28_00684 at 727.215 kb on - strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.276 kb on + strand, within PFR28_00684 at 727.276 kb on + strand, within PFR28_00684 at 727.276 kb on + strand, within PFR28_00684 at 727.276 kb on + strand, within PFR28_00684 at 727.276 kb on + strand, within PFR28_00684 at 727.276 kb on + strand, within PFR28_00684 at 727.276 kb on + strand, within PFR28_00684 at 727.276 kb on + strand, within PFR28_00684 at 727.276 kb on + strand, within PFR28_00684 at 727.276 kb on + strand, within PFR28_00684 at 727.276 kb on + strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.279 kb on + strand, within PFR28_00684 at 727.279 kb on + strand, within PFR28_00684 at 727.280 kb on - strand, within PFR28_00684 at 727.387 kb on + strand, within PFR28_00684 at 727.388 kb on - strand, within PFR28_00684 at 727.388 kb on - strand, within PFR28_00684 at 727.388 kb on - strand, within PFR28_00684 at 727.399 kb on + strand, within PFR28_00684 at 727.399 kb on + strand, within PFR28_00684 at 727.399 kb on + strand, within PFR28_00684 at 727.400 kb on - strand, within PFR28_00684 at 727.400 kb on - strand, within PFR28_00684 at 727.400 kb on - strand, within PFR28_00684 at 727.400 kb on - strand, within PFR28_00684 at 727.694 kb on + strand, within PFR28_00684 at 727.694 kb on + strand, within PFR28_00684 at 727.695 kb on - strand, within PFR28_00684 at 727.695 kb on - strand, within PFR28_00684 at 727.695 kb on - strand, within PFR28_00684 at 728.151 kb on + strand, within PFR28_00684 at 728.151 kb on + strand, within PFR28_00684 at 728.151 kb on + strand, within PFR28_00684 at 728.152 kb on - strand, within PFR28_00684 at 728.267 kb on - strand, within PFR28_00684 at 728.427 kb on + strand at 728.427 kb on + strand at 728.427 kb on + strand at 728.427 kb on + strand at 728.428 kb on - strand at 728.428 kb on - strand at 728.428 kb on - strand at 728.428 kb on - strand at 728.428 kb on - strand at 728.428 kb on - strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.448 kb on + strand at 728.448 kb on + strand at 728.448 kb on + strand at 728.448 kb on + strand at 728.448 kb on + strand at 728.448 kb on + strand at 728.448 kb on + strand at 728.448 kb on + strand at 728.448 kb on + strand at 728.448 kb on + strand at 728.448 kb on + strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.505 kb on + strand at 728.505 kb on + strand at 728.505 kb on + strand at 728.506 kb on - strand at 728.506 kb on - strand at 728.506 kb on - strand at 728.631 kb on + strand, within PFR28_00685 at 728.631 kb on + strand, within PFR28_00685 at 728.631 kb on + strand, within PFR28_00685 at 728.631 kb on + strand, within PFR28_00685 at 728.631 kb on + strand, within PFR28_00685 at 728.631 kb on + strand, within PFR28_00685 at 728.637 kb on + strand, within PFR28_00685 at 728.638 kb on - strand, within PFR28_00685 at 728.638 kb on - strand, within PFR28_00685 at 728.638 kb on - strand, within PFR28_00685 at 728.638 kb on - strand, within PFR28_00685 at 728.667 kb on + strand, within PFR28_00685 at 728.668 kb on - strand, within PFR28_00685 at 728.668 kb on - strand, within PFR28_00685 at 728.841 kb on + strand, within PFR28_00685 at 728.841 kb on + strand, within PFR28_00685 at 728.841 kb on + strand, within PFR28_00685 at 728.841 kb on + strand, within PFR28_00685 at 728.841 kb on + strand, within PFR28_00685 at 728.842 kb on - strand, within PFR28_00685 at 728.842 kb on - strand, within PFR28_00685 at 728.842 kb on - strand, within PFR28_00685 at 728.916 kb on + strand, within PFR28_00685 at 728.934 kb on + strand, within PFR28_00685 at 728.934 kb on + strand, within PFR28_00685 at 728.934 kb on + strand, within PFR28_00685 at 728.934 kb on + strand, within PFR28_00685 at 728.935 kb on - strand, within PFR28_00685 at 728.935 kb on - strand, within PFR28_00685 at 729.054 kb on + strand, within PFR28_00685 at 729.054 kb on + strand, within PFR28_00685 at 729.054 kb on + strand, within PFR28_00685 at 729.054 kb on + strand, within PFR28_00685 at 729.054 kb on + strand, within PFR28_00685 at 729.054 kb on + strand, within PFR28_00685 at 729.054 kb on + strand, within PFR28_00685 at 729.054 kb on + strand, within PFR28_00685 at 729.054 kb on + strand, within PFR28_00685 at 729.054 kb on + strand, within PFR28_00685 at 729.054 kb on + strand, within PFR28_00685 at 729.055 kb on - strand, within PFR28_00685 at 729.055 kb on - strand, within PFR28_00685 at 729.055 kb on - strand, within PFR28_00685 at 729.055 kb on - strand, within PFR28_00685 at 729.055 kb on - strand, within PFR28_00685 at 729.305 kb on - strand at 729.333 kb on + strand at 729.392 kb on + strand at 729.393 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Citric Acid (C) 10 mM remove 726,024 + PFR28_00682 0.44 -2.4 726,024 + PFR28_00682 0.44 -1.9 726,024 + PFR28_00682 0.44 -3.5 726,025 - PFR28_00682 0.45 +0.1 726,025 - PFR28_00682 0.45 -2.7 726,025 - PFR28_00682 0.45 +0.6 726,025 - PFR28_00682 0.45 +0.3 726,134 + -2.7 726,134 + -2.2 726,134 + -4.5 726,134 + -2.9 726,135 - -1.3 726,135 - -1.0 726,144 + -2.1 726,145 - -0.1 726,145 - -0.4 726,145 - -0.8 726,153 + -0.5 726,217 - -0.0 726,217 - +0.7 726,232 + -3.1 726,232 + -3.5 726,252 - -1.1 726,355 + -1.9 726,355 + -3.5 726,355 + -2.1 726,356 - -4.1 726,356 - -0.8 726,356 - -3.6 726,356 - -1.8 726,492 + PFR28_00683 0.20 -4.5 726,493 - PFR28_00683 0.20 -4.1 726,514 + PFR28_00683 0.23 -5.1 726,515 - PFR28_00683 0.23 -5.0 726,515 - PFR28_00683 0.23 -1.6 726,760 - PFR28_00683 0.60 -4.4 726,760 - PFR28_00683 0.60 -2.8 726,760 - PFR28_00683 0.60 -2.5 726,760 - PFR28_00683 0.60 -1.8 726,760 - PFR28_00683 0.60 -3.9 726,760 - PFR28_00683 0.60 -3.1 726,937 - PFR28_00683 0.86 -3.7 726,937 - PFR28_00683 0.86 -4.0 726,937 - PFR28_00683 0.86 -3.7 727,006 - -3.3 727,029 + -3.2 727,029 + +0.9 727,030 - -3.3 727,030 - -2.6 727,030 - -3.0 727,030 - -1.8 727,030 - -3.3 727,214 + PFR28_00684 0.14 -1.8 727,215 - PFR28_00684 0.14 -4.5 727,231 + PFR28_00684 0.15 -3.7 727,231 + PFR28_00684 0.15 -3.5 727,231 + PFR28_00684 0.15 -3.9 727,231 + PFR28_00684 0.15 -3.4 727,231 + PFR28_00684 0.15 -2.0 727,231 + PFR28_00684 0.15 -3.7 727,231 + PFR28_00684 0.15 -3.9 727,231 + PFR28_00684 0.15 -1.8 727,231 + PFR28_00684 0.15 -3.7 727,231 + PFR28_00684 0.15 -2.1 727,231 + PFR28_00684 0.15 -3.3 727,231 + PFR28_00684 0.15 -2.2 727,231 + PFR28_00684 0.15 -4.7 727,231 + PFR28_00684 0.15 -4.0 727,231 + PFR28_00684 0.15 -3.9 727,231 + PFR28_00684 0.15 -1.9 727,232 - PFR28_00684 0.15 -2.4 727,232 - PFR28_00684 0.15 -0.8 727,232 - PFR28_00684 0.15 -2.9 727,232 - PFR28_00684 0.15 -1.6 727,232 - PFR28_00684 0.15 -4.0 727,232 - PFR28_00684 0.15 -3.1 727,232 - PFR28_00684 0.15 -3.6 727,232 - PFR28_00684 0.15 -4.9 727,232 - PFR28_00684 0.15 -4.9 727,232 - PFR28_00684 0.15 -4.3 727,232 - PFR28_00684 0.15 -4.4 727,232 - PFR28_00684 0.15 -5.1 727,232 - PFR28_00684 0.15 -3.1 727,232 - PFR28_00684 0.15 -2.9 727,232 - PFR28_00684 0.15 -4.0 727,232 - PFR28_00684 0.15 -5.6 727,276 + PFR28_00684 0.18 -3.6 727,276 + PFR28_00684 0.18 -4.5 727,276 + PFR28_00684 0.18 -0.8 727,276 + PFR28_00684 0.18 -2.7 727,276 + PFR28_00684 0.18 -3.2 727,276 + PFR28_00684 0.18 -3.1 727,276 + PFR28_00684 0.18 -4.1 727,276 + PFR28_00684 0.18 -2.3 727,276 + PFR28_00684 0.18 -3.2 727,276 + PFR28_00684 0.18 -4.7 727,276 + PFR28_00684 0.18 -2.4 727,277 - PFR28_00684 0.18 -3.4 727,277 - PFR28_00684 0.18 -4.2 727,277 - PFR28_00684 0.18 -3.7 727,277 - PFR28_00684 0.18 -3.3 727,277 - PFR28_00684 0.18 -3.2 727,277 - PFR28_00684 0.18 -3.6 727,277 - PFR28_00684 0.18 -4.4 727,277 - PFR28_00684 0.18 -3.3 727,277 - PFR28_00684 0.18 -4.0 727,277 - PFR28_00684 0.18 -5.0 727,277 - PFR28_00684 0.18 -1.6 727,277 - PFR28_00684 0.18 -2.2 727,277 - PFR28_00684 0.18 -4.5 727,277 - PFR28_00684 0.18 -4.9 727,277 - PFR28_00684 0.18 -1.2 727,277 - PFR28_00684 0.18 -2.8 727,277 - PFR28_00684 0.18 -4.3 727,277 - PFR28_00684 0.18 -3.9 727,279 + PFR28_00684 0.18 -3.0 727,279 + PFR28_00684 0.18 -3.1 727,280 - PFR28_00684 0.18 -4.0 727,387 + PFR28_00684 0.26 -3.7 727,388 - PFR28_00684 0.26 -4.0 727,388 - PFR28_00684 0.26 -3.7 727,388 - PFR28_00684 0.26 -3.7 727,399 + PFR28_00684 0.27 -4.2 727,399 + PFR28_00684 0.27 -4.4 727,399 + PFR28_00684 0.27 -5.0 727,400 - PFR28_00684 0.27 -2.0 727,400 - PFR28_00684 0.27 -2.1 727,400 - PFR28_00684 0.27 -1.6 727,400 - PFR28_00684 0.27 -2.1 727,694 + PFR28_00684 0.48 -5.1 727,694 + PFR28_00684 0.48 -2.3 727,695 - PFR28_00684 0.48 -3.2 727,695 - PFR28_00684 0.48 -3.0 727,695 - PFR28_00684 0.48 -1.4 728,151 + PFR28_00684 0.81 -2.4 728,151 + PFR28_00684 0.81 -6.1 728,151 + PFR28_00684 0.81 -4.3 728,152 - PFR28_00684 0.81 +1.2 728,267 - PFR28_00684 0.89 -2.1 728,427 + -0.1 728,427 + +0.5 728,427 + -0.6 728,427 + -0.3 728,428 - +1.5 728,428 - -0.3 728,428 - +0.2 728,428 - +0.6 728,428 - +0.9 728,428 - +0.5 728,446 + -1.1 728,446 + +0.6 728,446 + +0.2 728,446 + +0.6 728,446 + -0.2 728,446 + -2.6 728,446 + +0.3 728,446 + -0.4 728,446 + +0.5 728,446 + +0.4 728,446 + +0.1 728,446 + +0.3 728,446 + -0.3 728,446 + +1.2 728,446 + +0.6 728,446 + +0.6 728,446 + +0.3 728,446 + +0.4 728,446 + +0.6 728,447 - +0.3 728,447 - -0.4 728,447 - -0.2 728,447 - +0.2 728,447 - +0.3 728,447 - +2.2 728,447 - +0.1 728,447 - +0.0 728,447 - +0.1 728,447 - -0.9 728,447 - +0.2 728,447 - -1.2 728,447 - +0.3 728,447 - +0.5 728,447 - -0.4 728,448 + +0.5 728,448 + +0.2 728,448 + -0.0 728,448 + -1.6 728,448 + -0.5 728,448 + +0.3 728,448 + -0.4 728,448 + +0.7 728,448 + +0.3 728,448 + +1.1 728,448 + +0.3 728,449 - -1.0 728,449 - +0.1 728,449 - -1.9 728,449 - +0.1 728,449 - +1.2 728,449 - +0.7 728,449 - +0.3 728,449 - +1.4 728,449 - -1.4 728,449 - +0.4 728,449 - -0.2 728,449 - -0.5 728,449 - -0.2 728,449 - +0.4 728,449 - -0.8 728,449 - -0.3 728,449 - -0.1 728,449 - -0.6 728,505 + -0.0 728,505 + +0.2 728,505 + -0.1 728,506 - -0.1 728,506 - -1.1 728,506 - +0.2 728,631 + PFR28_00685 0.16 -0.1 728,631 + PFR28_00685 0.16 +1.1 728,631 + PFR28_00685 0.16 +0.3 728,631 + PFR28_00685 0.16 -0.7 728,631 + PFR28_00685 0.16 -0.3 728,631 + PFR28_00685 0.16 +0.3 728,637 + PFR28_00685 0.17 +0.1 728,638 - PFR28_00685 0.17 -1.4 728,638 - PFR28_00685 0.17 -0.8 728,638 - PFR28_00685 0.17 -1.2 728,638 - PFR28_00685 0.17 -0.1 728,667 + PFR28_00685 0.20 +1.1 728,668 - PFR28_00685 0.20 +0.0 728,668 - PFR28_00685 0.20 +1.1 728,841 + PFR28_00685 0.41 +1.3 728,841 + PFR28_00685 0.41 +0.1 728,841 + PFR28_00685 0.41 +0.7 728,841 + PFR28_00685 0.41 -0.2 728,841 + PFR28_00685 0.41 +0.3 728,842 - PFR28_00685 0.41 -0.1 728,842 - PFR28_00685 0.41 +0.2 728,842 - PFR28_00685 0.41 -0.0 728,916 + PFR28_00685 0.50 +0.1 728,934 + PFR28_00685 0.53 +0.1 728,934 + PFR28_00685 0.53 -0.7 728,934 + PFR28_00685 0.53 +0.1 728,934 + PFR28_00685 0.53 -0.4 728,935 - PFR28_00685 0.53 -0.8 728,935 - PFR28_00685 0.53 +0.8 729,054 + PFR28_00685 0.67 -0.0 729,054 + PFR28_00685 0.67 +0.0 729,054 + PFR28_00685 0.67 -1.0 729,054 + PFR28_00685 0.67 -0.3 729,054 + PFR28_00685 0.67 -0.9 729,054 + PFR28_00685 0.67 -0.1 729,054 + PFR28_00685 0.67 +0.8 729,054 + PFR28_00685 0.67 -0.1 729,054 + PFR28_00685 0.67 -1.0 729,054 + PFR28_00685 0.67 +0.4 729,054 + PFR28_00685 0.67 -0.3 729,055 - PFR28_00685 0.67 +0.3 729,055 - PFR28_00685 0.67 -0.2 729,055 - PFR28_00685 0.67 -0.1 729,055 - PFR28_00685 0.67 +0.4 729,055 - PFR28_00685 0.67 -0.4 729,305 - +1.0 729,333 + +0.2 729,392 + -0.6 729,393 - -0.7
Or see this region's nucleotide sequence