Strain Fitness in Pseudomonas sp. RS175 around PFR28_00304

Experiment: Citric Acid (C) 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFR28_00302 and PFR28_00303 overlap by 13 nucleotidesPFR28_00303 and PFR28_00304 are separated by 67 nucleotidesPFR28_00304 and PFR28_00305 are separated by 120 nucleotides PFR28_00302: PFR28_00302 - Methionine aminotransferase, at 329,930 to 331,078 _00302 PFR28_00303: PFR28_00303 - Omega-amidase YafV, at 331,066 to 331,857 _00303 PFR28_00304: PFR28_00304 - hypothetical protein, at 331,925 to 332,332 _00304 PFR28_00305: PFR28_00305 - 2-isopropylmalate synthase, at 332,453 to 334,132 _00305 Position (kb) 331 332 333Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 330.980 kb on + strandat 330.981 kb on - strandat 330.981 kb on - strandat 331.065 kb on + strandat 331.065 kb on + strandat 331.066 kb on - strandat 331.080 kb on + strandat 331.080 kb on + strandat 331.080 kb on + strandat 331.081 kb on - strandat 331.081 kb on - strandat 331.081 kb on - strandat 331.081 kb on - strandat 331.081 kb on - strandat 331.081 kb on - strandat 331.269 kb on + strand, within PFR28_00303at 331.402 kb on + strand, within PFR28_00303at 331.402 kb on + strand, within PFR28_00303at 331.403 kb on - strand, within PFR28_00303at 331.403 kb on - strand, within PFR28_00303at 331.403 kb on - strand, within PFR28_00303at 331.447 kb on + strand, within PFR28_00303at 331.448 kb on - strand, within PFR28_00303at 331.448 kb on - strand, within PFR28_00303at 331.448 kb on - strand, within PFR28_00303at 331.513 kb on + strand, within PFR28_00303at 331.514 kb on - strand, within PFR28_00303at 331.570 kb on + strand, within PFR28_00303at 331.571 kb on - strand, within PFR28_00303at 331.571 kb on - strand, within PFR28_00303at 331.648 kb on + strand, within PFR28_00303at 331.648 kb on + strand, within PFR28_00303at 331.648 kb on + strand, within PFR28_00303at 331.648 kb on + strand, within PFR28_00303at 331.649 kb on - strand, within PFR28_00303at 331.649 kb on - strand, within PFR28_00303at 331.649 kb on - strand, within PFR28_00303at 331.652 kb on + strand, within PFR28_00303at 331.652 kb on + strand, within PFR28_00303at 331.653 kb on - strand, within PFR28_00303at 331.697 kb on - strand, within PFR28_00303at 331.933 kb on + strandat 331.933 kb on + strandat 331.934 kb on - strandat 331.975 kb on + strand, within PFR28_00304at 331.993 kb on + strand, within PFR28_00304at 331.994 kb on - strand, within PFR28_00304at 332.292 kb on + strandat 332.546 kb on + strandat 332.589 kb on + strandat 332.589 kb on + strandat 332.589 kb on + strandat 332.590 kb on - strandat 332.590 kb on - strandat 332.590 kb on - strandat 332.590 kb on - strandat 332.590 kb on - strandat 332.637 kb on + strand, within PFR28_00305at 332.637 kb on + strand, within PFR28_00305at 332.638 kb on - strand, within PFR28_00305at 332.638 kb on - strand, within PFR28_00305at 332.638 kb on - strand, within PFR28_00305at 332.805 kb on + strand, within PFR28_00305at 332.947 kb on - strand, within PFR28_00305at 332.964 kb on + strand, within PFR28_00305at 332.964 kb on + strand, within PFR28_00305at 333.015 kb on + strand, within PFR28_00305at 333.016 kb on - strand, within PFR28_00305at 333.016 kb on - strand, within PFR28_00305at 333.016 kb on - strand, within PFR28_00305at 333.016 kb on - strand, within PFR28_00305at 333.153 kb on + strand, within PFR28_00305at 333.153 kb on + strand, within PFR28_00305at 333.153 kb on + strand, within PFR28_00305at 333.153 kb on + strand, within PFR28_00305at 333.153 kb on + strand, within PFR28_00305at 333.153 kb on + strand, within PFR28_00305at 333.153 kb on + strand, within PFR28_00305at 333.153 kb on + strand, within PFR28_00305at 333.154 kb on - strand, within PFR28_00305at 333.154 kb on - strand, within PFR28_00305at 333.154 kb on - strand, within PFR28_00305at 333.154 kb on - strand, within PFR28_00305at 333.154 kb on - strand, within PFR28_00305at 333.154 kb on - strand, within PFR28_00305at 333.255 kb on + strand, within PFR28_00305at 333.255 kb on + strand, within PFR28_00305at 333.256 kb on - strand, within PFR28_00305at 333.256 kb on - strand, within PFR28_00305at 333.256 kb on - strand, within PFR28_00305at 333.256 kb on - strand, within PFR28_00305

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Per-strain Table

Position Strand Gene LocusTag Fraction Citric Acid (C) 10 mM
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330,980 + -0.2
330,981 - -0.1
330,981 - +0.5
331,065 + -0.7
331,065 + +0.2
331,066 - -0.8
331,080 + +0.1
331,080 + +0.3
331,080 + +0.1
331,081 - +0.4
331,081 - -0.8
331,081 - -0.4
331,081 - +0.4
331,081 - +1.2
331,081 - -1.1
331,269 + PFR28_00303 0.26 -0.1
331,402 + PFR28_00303 0.42 +0.8
331,402 + PFR28_00303 0.42 -2.4
331,403 - PFR28_00303 0.43 -0.4
331,403 - PFR28_00303 0.43 +0.2
331,403 - PFR28_00303 0.43 -0.8
331,447 + PFR28_00303 0.48 +1.2
331,448 - PFR28_00303 0.48 +1.8
331,448 - PFR28_00303 0.48 +0.2
331,448 - PFR28_00303 0.48 -0.9
331,513 + PFR28_00303 0.56 -0.2
331,514 - PFR28_00303 0.57 +0.1
331,570 + PFR28_00303 0.64 +0.4
331,571 - PFR28_00303 0.64 -1.1
331,571 - PFR28_00303 0.64 -0.2
331,648 + PFR28_00303 0.73 +1.6
331,648 + PFR28_00303 0.73 -0.7
331,648 + PFR28_00303 0.73 -0.8
331,648 + PFR28_00303 0.73 -0.7
331,649 - PFR28_00303 0.74 +0.3
331,649 - PFR28_00303 0.74 -0.4
331,649 - PFR28_00303 0.74 -0.8
331,652 + PFR28_00303 0.74 +0.3
331,652 + PFR28_00303 0.74 +0.0
331,653 - PFR28_00303 0.74 -0.7
331,697 - PFR28_00303 0.80 +0.3
331,933 + +0.7
331,933 + -0.6
331,934 - +0.2
331,975 + PFR28_00304 0.12 -0.7
331,993 + PFR28_00304 0.17 -0.3
331,994 - PFR28_00304 0.17 -1.9
332,292 + -0.1
332,546 + +1.2
332,589 + -2.5
332,589 + -3.5
332,589 + -4.1
332,590 - -2.4
332,590 - -2.3
332,590 - -2.2
332,590 - -2.6
332,590 - -2.1
332,637 + PFR28_00305 0.11 -0.7
332,637 + PFR28_00305 0.11 +0.1
332,638 - PFR28_00305 0.11 -1.1
332,638 - PFR28_00305 0.11 +1.2
332,638 - PFR28_00305 0.11 +1.2
332,805 + PFR28_00305 0.21 -1.2
332,947 - PFR28_00305 0.29 -2.6
332,964 + PFR28_00305 0.30 -0.8
332,964 + PFR28_00305 0.30 -4.0
333,015 + PFR28_00305 0.33 -3.6
333,016 - PFR28_00305 0.34 -1.4
333,016 - PFR28_00305 0.34 -3.7
333,016 - PFR28_00305 0.34 -5.0
333,016 - PFR28_00305 0.34 -2.1
333,153 + PFR28_00305 0.42 -1.1
333,153 + PFR28_00305 0.42 -3.7
333,153 + PFR28_00305 0.42 -3.4
333,153 + PFR28_00305 0.42 -1.9
333,153 + PFR28_00305 0.42 -3.7
333,153 + PFR28_00305 0.42 -2.7
333,153 + PFR28_00305 0.42 -3.0
333,153 + PFR28_00305 0.42 -3.5
333,154 - PFR28_00305 0.42 -3.9
333,154 - PFR28_00305 0.42 -2.8
333,154 - PFR28_00305 0.42 -3.2
333,154 - PFR28_00305 0.42 -2.6
333,154 - PFR28_00305 0.42 -2.9
333,154 - PFR28_00305 0.42 -3.3
333,255 + PFR28_00305 0.48 -4.2
333,255 + PFR28_00305 0.48 -2.6
333,256 - PFR28_00305 0.48 -2.2
333,256 - PFR28_00305 0.48 -4.2
333,256 - PFR28_00305 0.48 -4.3
333,256 - PFR28_00305 0.48 -1.1

Or see this region's nucleotide sequence