Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt atpH and atpF are separated by 14 nucleotides atpF and atpE are separated by 61 nucleotides atpE and atpB are separated by 46 nucleotides atpB and atpI are separated by 8 nucleotides
b3735: atpH - F0F1 ATP synthase subunit delta (NCBI), at 3,917,893 to 3,918,426
atpH
b3736: atpF - F0F1 ATP synthase subunit B (NCBI), at 3,918,441 to 3,918,911
atpF
b3737: atpE - F0F1 ATP synthase subunit C (NCBI), at 3,918,973 to 3,919,212
atpE
b3738: atpB - F0F1 ATP synthase subunit A (NCBI), at 3,919,259 to 3,920,074
atpB
b3739: atpI - membrane-bound ATP synthase, dispensable protein, affects expression of atpB (VIMSS), at 3,920,083 to 3,920,463
atpI
Position (kb)
3919
3920
3921 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3918.286 kb on - strand, within atpH at 3918.435 kb on - strand at 3918.440 kb on - strand at 3918.444 kb on - strand at 3918.444 kb on - strand at 3918.447 kb on - strand at 3918.447 kb on - strand at 3918.454 kb on - strand at 3918.454 kb on - strand at 3919.227 kb on - strand at 3919.248 kb on - strand at 3919.265 kb on - strand at 3919.265 kb on - strand at 3920.048 kb on - strand at 3920.063 kb on - strand at 3920.063 kb on - strand at 3920.119 kb on - strand at 3920.212 kb on - strand, within atpI at 3920.212 kb on - strand, within atpI at 3920.223 kb on - strand, within atpI at 3920.270 kb on - strand, within atpI at 3920.270 kb on - strand, within atpI at 3920.362 kb on - strand, within atpI at 3920.362 kb on - strand, within atpI at 3920.362 kb on - strand, within atpI at 3920.365 kb on - strand, within atpI at 3920.375 kb on - strand, within atpI at 3920.408 kb on - strand, within atpI at 3920.410 kb on - strand, within atpI at 3920.412 kb on - strand, within atpI at 3920.425 kb on - strand, within atpI at 3920.462 kb on - strand at 3920.488 kb on - strand at 3920.511 kb on - strand at 3920.514 kb on - strand at 3920.533 kb on - strand at 3920.538 kb on - strand at 3920.558 kb on - strand at 3920.561 kb on - strand at 3920.594 kb on - strand at 3920.594 kb on - strand at 3920.638 kb on - strand at 3920.638 kb on - strand at 3920.638 kb on - strand at 3920.638 kb on - strand at 3920.641 kb on + strand at 3920.649 kb on - strand at 3920.651 kb on + strand at 3920.658 kb on - strand at 3920.675 kb on + strand at 3920.675 kb on + strand at 3920.696 kb on - strand at 3920.696 kb on - strand at 3920.698 kb on + strand at 3920.699 kb on + strand at 3920.699 kb on + strand at 3920.707 kb on + strand at 3920.707 kb on - strand at 3920.717 kb on + strand at 3920.722 kb on + strand at 3920.722 kb on + strand at 3920.722 kb on + strand at 3920.725 kb on - strand at 3920.726 kb on - strand at 3920.726 kb on - strand at 3920.729 kb on - strand at 3920.729 kb on - strand at 3920.751 kb on - strand at 3920.768 kb on + strand at 3920.773 kb on + strand at 3920.867 kb on + strand at 3920.877 kb on + strand at 3920.885 kb on - strand at 3920.885 kb on - strand at 3920.920 kb on - strand at 3920.920 kb on - strand at 3920.963 kb on + strand at 3920.963 kb on + strand at 3920.982 kb on - strand at 3920.985 kb on + strand at 3920.992 kb on + strand at 3920.997 kb on + strand at 3920.997 kb on + strand at 3921.014 kb on - strand at 3921.014 kb on - strand at 3921.014 kb on - strand at 3921.014 kb on - strand at 3921.036 kb on + strand at 3921.039 kb on + strand at 3921.039 kb on + strand at 3921.047 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 3,918,286 - atpH b3735 0.74 +0.2 3,918,435 - -0.9 3,918,440 - +0.7 3,918,444 - -0.6 3,918,444 - -0.2 3,918,447 - +1.0 3,918,447 - +0.6 3,918,454 - -1.4 3,918,454 - +0.4 3,919,227 - +0.4 3,919,248 - -0.4 3,919,265 - -0.6 3,919,265 - +0.4 3,920,048 - +1.5 3,920,063 - +0.6 3,920,063 - +1.5 3,920,119 - +0.4 3,920,212 - atpI b3739 0.34 -0.8 3,920,212 - atpI b3739 0.34 +0.6 3,920,223 - atpI b3739 0.37 +0.9 3,920,270 - atpI b3739 0.49 +1.2 3,920,270 - atpI b3739 0.49 +0.6 3,920,362 - atpI b3739 0.73 -1.2 3,920,362 - atpI b3739 0.73 -0.7 3,920,362 - atpI b3739 0.73 +0.5 3,920,365 - atpI b3739 0.74 +1.2 3,920,375 - atpI b3739 0.77 +0.1 3,920,408 - atpI b3739 0.85 +0.2 3,920,410 - atpI b3739 0.86 +0.6 3,920,412 - atpI b3739 0.86 -0.1 3,920,425 - atpI b3739 0.90 +0.6 3,920,462 - -0.8 3,920,488 - +0.3 3,920,511 - -0.1 3,920,514 - +0.0 3,920,533 - +0.7 3,920,538 - +0.6 3,920,558 - -0.3 3,920,561 - -0.6 3,920,594 - -0.1 3,920,594 - -0.1 3,920,638 - +0.2 3,920,638 - +0.8 3,920,638 - -1.1 3,920,638 - +0.7 3,920,641 + -0.3 3,920,649 - +0.7 3,920,651 + -0.4 3,920,658 - +0.9 3,920,675 + +1.2 3,920,675 + +3.5 3,920,696 - +1.4 3,920,696 - +0.5 3,920,698 + +0.3 3,920,699 + +0.3 3,920,699 + -2.0 3,920,707 + -0.1 3,920,707 - +3.5 3,920,717 + -1.4 3,920,722 + -1.0 3,920,722 + +0.7 3,920,722 + -0.3 3,920,725 - -0.4 3,920,726 - +0.7 3,920,726 - -0.9 3,920,729 - -0.4 3,920,729 - +0.6 3,920,751 - -0.0 3,920,768 + +0.8 3,920,773 + -0.4 3,920,867 + +0.2 3,920,877 + +0.5 3,920,885 - +2.2 3,920,885 - -2.0 3,920,920 - -0.1 3,920,920 - -1.0 3,920,963 + +0.5 3,920,963 + +0.4 3,920,982 - +0.5 3,920,985 + +0.9 3,920,992 + -1.0 3,920,997 + +2.2 3,920,997 + +0.1 3,921,014 - +0.1 3,921,014 - +0.2 3,921,014 - -0.1 3,921,014 - -0.4 3,921,036 + +1.0 3,921,039 + +0.5 3,921,039 + -0.0 3,921,047 - +0.7
Or see this region's nucleotide sequence