Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cpdA and yqiB are separated by 24 nucleotides yqiB and nudF are separated by 0 nucleotides nudF and tolC are separated by 204 nucleotides
b3032: cpdA - cyclic 3',5'-adenosine monophosphate phosphodiesterase (NCBI), at 3,174,028 to 3,174,855
cpdA
b3033: yqiB - predicted dehydrogenase (NCBI), at 3,174,880 to 3,175,302
yqiB
b3034: nudF - ADP-ribose pyrophosphatase (NCBI), at 3,175,303 to 3,175,932
nudF
b3035: tolC - outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS), at 3,176,137 to 3,177,618
tolC
Position (kb)
3175
3176 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3174.303 kb on - strand, within cpdA at 3174.332 kb on + strand, within cpdA at 3174.332 kb on + strand, within cpdA at 3174.373 kb on - strand, within cpdA at 3174.373 kb on - strand, within cpdA at 3174.405 kb on + strand, within cpdA at 3174.433 kb on - strand, within cpdA at 3174.457 kb on + strand, within cpdA at 3174.503 kb on + strand, within cpdA at 3174.506 kb on + strand, within cpdA at 3174.647 kb on + strand, within cpdA at 3174.694 kb on + strand, within cpdA at 3174.749 kb on + strand, within cpdA at 3174.749 kb on + strand, within cpdA at 3174.749 kb on + strand, within cpdA at 3174.791 kb on - strand at 3174.825 kb on - strand at 3174.825 kb on - strand at 3174.905 kb on - strand at 3174.915 kb on - strand at 3174.927 kb on - strand, within yqiB at 3175.065 kb on + strand, within yqiB at 3175.116 kb on + strand, within yqiB at 3175.126 kb on + strand, within yqiB at 3175.171 kb on - strand, within yqiB at 3175.226 kb on + strand, within yqiB at 3175.399 kb on - strand, within nudF at 3175.469 kb on - strand, within nudF at 3175.494 kb on - strand, within nudF at 3175.494 kb on - strand, within nudF at 3175.528 kb on - strand, within nudF at 3175.577 kb on + strand, within nudF at 3175.585 kb on + strand, within nudF at 3175.590 kb on - strand, within nudF at 3175.662 kb on + strand, within nudF at 3175.679 kb on + strand, within nudF at 3175.679 kb on + strand, within nudF at 3175.752 kb on - strand, within nudF at 3175.798 kb on + strand, within nudF at 3175.818 kb on - strand, within nudF at 3175.823 kb on - strand, within nudF at 3175.871 kb on - strand at 3176.030 kb on - strand at 3176.031 kb on + strand at 3176.119 kb on - strand at 3176.119 kb on - strand at 3176.173 kb on - strand at 3176.173 kb on - strand at 3176.174 kb on + strand at 3176.182 kb on - strand at 3176.208 kb on - strand at 3176.271 kb on + strand at 3176.271 kb on + strand at 3176.304 kb on + strand, within tolC at 3176.309 kb on + strand, within tolC at 3176.309 kb on + strand, within tolC at 3176.349 kb on - strand, within tolC at 3176.378 kb on + strand, within tolC at 3176.493 kb on + strand, within tolC at 3176.526 kb on - strand, within tolC at 3176.571 kb on + strand, within tolC at 3176.573 kb on + strand, within tolC at 3176.581 kb on - strand, within tolC at 3176.635 kb on + strand, within tolC at 3176.638 kb on - strand, within tolC at 3176.654 kb on - strand, within tolC at 3176.680 kb on - strand, within tolC at 3176.680 kb on - strand, within tolC at 3176.682 kb on + strand, within tolC at 3176.692 kb on - strand, within tolC at 3176.716 kb on + strand, within tolC at 3176.716 kb on + strand, within tolC at 3176.844 kb on - strand, within tolC at 3176.844 kb on - strand, within tolC at 3176.869 kb on - strand, within tolC at 3176.869 kb on - strand, within tolC at 3176.887 kb on + strand, within tolC at 3176.887 kb on + strand, within tolC at 3176.887 kb on + strand, within tolC at 3176.932 kb on + strand, within tolC
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 3,174,303 - cpdA b3032 0.33 +0.6 3,174,332 + cpdA b3032 0.37 -1.0 3,174,332 + cpdA b3032 0.37 +0.3 3,174,373 - cpdA b3032 0.42 +3.2 3,174,373 - cpdA b3032 0.42 +0.6 3,174,405 + cpdA b3032 0.46 +0.2 3,174,433 - cpdA b3032 0.49 +2.0 3,174,457 + cpdA b3032 0.52 -0.1 3,174,503 + cpdA b3032 0.57 +0.1 3,174,506 + cpdA b3032 0.58 +0.3 3,174,647 + cpdA b3032 0.75 -0.5 3,174,694 + cpdA b3032 0.80 -1.0 3,174,749 + cpdA b3032 0.87 -0.9 3,174,749 + cpdA b3032 0.87 +0.6 3,174,749 + cpdA b3032 0.87 -0.4 3,174,791 - -0.8 3,174,825 - -0.8 3,174,825 - +1.0 3,174,905 - -0.4 3,174,915 - +2.0 3,174,927 - yqiB b3033 0.11 +1.5 3,175,065 + yqiB b3033 0.44 -0.4 3,175,116 + yqiB b3033 0.56 +0.1 3,175,126 + yqiB b3033 0.58 +0.3 3,175,171 - yqiB b3033 0.69 +0.9 3,175,226 + yqiB b3033 0.82 +0.0 3,175,399 - nudF b3034 0.15 +0.7 3,175,469 - nudF b3034 0.26 +1.1 3,175,494 - nudF b3034 0.30 -0.8 3,175,494 - nudF b3034 0.30 +2.2 3,175,528 - nudF b3034 0.36 +2.0 3,175,577 + nudF b3034 0.43 +1.7 3,175,585 + nudF b3034 0.45 +0.2 3,175,590 - nudF b3034 0.46 +1.4 3,175,662 + nudF b3034 0.57 +2.6 3,175,679 + nudF b3034 0.60 +0.6 3,175,679 + nudF b3034 0.60 +0.6 3,175,752 - nudF b3034 0.71 +1.4 3,175,798 + nudF b3034 0.79 -0.6 3,175,818 - nudF b3034 0.82 +1.5 3,175,823 - nudF b3034 0.83 +0.8 3,175,871 - +1.6 3,176,030 - +1.0 3,176,031 + +0.3 3,176,119 - +1.5 3,176,119 - +1.6 3,176,173 - +1.3 3,176,173 - +0.4 3,176,174 + +0.7 3,176,182 - +2.6 3,176,208 - +1.9 3,176,271 + +0.8 3,176,271 + +0.2 3,176,304 + tolC b3035 0.11 +0.7 3,176,309 + tolC b3035 0.12 +1.6 3,176,309 + tolC b3035 0.12 +2.2 3,176,349 - tolC b3035 0.14 +1.0 3,176,378 + tolC b3035 0.16 -0.5 3,176,493 + tolC b3035 0.24 -0.6 3,176,526 - tolC b3035 0.26 +1.1 3,176,571 + tolC b3035 0.29 -0.2 3,176,573 + tolC b3035 0.29 -0.8 3,176,581 - tolC b3035 0.30 +1.6 3,176,635 + tolC b3035 0.34 +0.3 3,176,638 - tolC b3035 0.34 -0.7 3,176,654 - tolC b3035 0.35 +1.7 3,176,680 - tolC b3035 0.37 +1.6 3,176,680 - tolC b3035 0.37 +1.0 3,176,682 + tolC b3035 0.37 +2.2 3,176,692 - tolC b3035 0.37 +0.7 3,176,716 + tolC b3035 0.39 +0.3 3,176,716 + tolC b3035 0.39 -0.8 3,176,844 - tolC b3035 0.48 +0.4 3,176,844 - tolC b3035 0.48 +0.6 3,176,869 - tolC b3035 0.49 -0.0 3,176,869 - tolC b3035 0.49 +2.0 3,176,887 + tolC b3035 0.51 -0.9 3,176,887 + tolC b3035 0.51 +2.0 3,176,887 + tolC b3035 0.51 +1.2 3,176,932 + tolC b3035 0.54 +0.8
Or see this region's nucleotide sequence