Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt parE and yqiA are separated by 28 nucleotides yqiA and cpdA overlap by 1 nucleotides cpdA and yqiB are separated by 24 nucleotides
b3030: parE - DNA topoisomerase IV subunit B (NCBI), at 3,171,526 to 3,173,418
parE
b3031: yqiA - predicted esterase (NCBI), at 3,173,447 to 3,174,028
yqiA
b3032: cpdA - cyclic 3',5'-adenosine monophosphate phosphodiesterase (NCBI), at 3,174,028 to 3,174,855
cpdA
b3033: yqiB - predicted dehydrogenase (NCBI), at 3,174,880 to 3,175,302
yqiB
Position (kb)
3173
3174
3175 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3173.456 kb on + strand at 3173.509 kb on - strand, within yqiA at 3173.533 kb on - strand, within yqiA at 3173.571 kb on + strand, within yqiA at 3173.682 kb on + strand, within yqiA at 3173.697 kb on + strand, within yqiA at 3173.705 kb on - strand, within yqiA at 3173.705 kb on - strand, within yqiA at 3173.705 kb on - strand, within yqiA at 3173.705 kb on - strand, within yqiA at 3173.705 kb on - strand, within yqiA at 3173.724 kb on - strand, within yqiA at 3173.762 kb on + strand, within yqiA at 3173.802 kb on + strand, within yqiA at 3173.805 kb on + strand, within yqiA at 3173.835 kb on + strand, within yqiA at 3173.835 kb on + strand, within yqiA at 3173.999 kb on - strand at 3174.018 kb on + strand at 3174.018 kb on + strand at 3174.070 kb on + strand at 3174.070 kb on + strand at 3174.125 kb on + strand, within cpdA at 3174.140 kb on + strand, within cpdA at 3174.150 kb on + strand, within cpdA at 3174.156 kb on + strand, within cpdA at 3174.178 kb on - strand, within cpdA at 3174.221 kb on - strand, within cpdA at 3174.271 kb on - strand, within cpdA at 3174.271 kb on - strand, within cpdA at 3174.303 kb on - strand, within cpdA at 3174.332 kb on + strand, within cpdA at 3174.332 kb on + strand, within cpdA at 3174.373 kb on - strand, within cpdA at 3174.373 kb on - strand, within cpdA at 3174.405 kb on + strand, within cpdA at 3174.433 kb on - strand, within cpdA at 3174.457 kb on + strand, within cpdA at 3174.503 kb on + strand, within cpdA at 3174.506 kb on + strand, within cpdA at 3174.647 kb on + strand, within cpdA at 3174.694 kb on + strand, within cpdA at 3174.749 kb on + strand, within cpdA at 3174.749 kb on + strand, within cpdA at 3174.749 kb on + strand, within cpdA at 3174.791 kb on - strand at 3174.825 kb on - strand at 3174.825 kb on - strand at 3174.905 kb on - strand at 3174.915 kb on - strand at 3174.927 kb on - strand, within yqiB
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 3,173,456 + -0.6 3,173,509 - yqiA b3031 0.11 -1.4 3,173,533 - yqiA b3031 0.15 +0.6 3,173,571 + yqiA b3031 0.21 -1.9 3,173,682 + yqiA b3031 0.40 -0.3 3,173,697 + yqiA b3031 0.43 +1.0 3,173,705 - yqiA b3031 0.44 -0.8 3,173,705 - yqiA b3031 0.44 +2.0 3,173,705 - yqiA b3031 0.44 -0.0 3,173,705 - yqiA b3031 0.44 -0.6 3,173,705 - yqiA b3031 0.44 -0.4 3,173,724 - yqiA b3031 0.48 -0.5 3,173,762 + yqiA b3031 0.54 -0.6 3,173,802 + yqiA b3031 0.61 +2.0 3,173,805 + yqiA b3031 0.62 -0.7 3,173,835 + yqiA b3031 0.67 -1.4 3,173,835 + yqiA b3031 0.67 -0.4 3,173,999 - +1.0 3,174,018 + +0.4 3,174,018 + +0.4 3,174,070 + -1.0 3,174,070 + -0.7 3,174,125 + cpdA b3032 0.12 -0.7 3,174,140 + cpdA b3032 0.14 -0.0 3,174,150 + cpdA b3032 0.15 -0.6 3,174,156 + cpdA b3032 0.15 -0.1 3,174,178 - cpdA b3032 0.18 +2.6 3,174,221 - cpdA b3032 0.23 -0.1 3,174,271 - cpdA b3032 0.29 +1.1 3,174,271 - cpdA b3032 0.29 +1.7 3,174,303 - cpdA b3032 0.33 +0.6 3,174,332 + cpdA b3032 0.37 -1.0 3,174,332 + cpdA b3032 0.37 +0.3 3,174,373 - cpdA b3032 0.42 +3.2 3,174,373 - cpdA b3032 0.42 +0.6 3,174,405 + cpdA b3032 0.46 +0.2 3,174,433 - cpdA b3032 0.49 +2.0 3,174,457 + cpdA b3032 0.52 -0.1 3,174,503 + cpdA b3032 0.57 +0.1 3,174,506 + cpdA b3032 0.58 +0.3 3,174,647 + cpdA b3032 0.75 -0.5 3,174,694 + cpdA b3032 0.80 -1.0 3,174,749 + cpdA b3032 0.87 -0.9 3,174,749 + cpdA b3032 0.87 +0.6 3,174,749 + cpdA b3032 0.87 -0.4 3,174,791 - -0.8 3,174,825 - -0.8 3,174,825 - +1.0 3,174,905 - -0.4 3,174,915 - +2.0 3,174,927 - yqiB b3033 0.11 +1.5
Or see this region's nucleotide sequence