Strain Fitness in Escherichia coli BW25113 around b1877

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntargS and yecT are separated by 176 nucleotidesyecT and flhE are separated by 119 nucleotidesflhE and flhA overlap by 1 nucleotides b1876: argS - arginyl-tRNA synthetase (NCBI), at 1,958,086 to 1,959,819 argS b1877: yecT - orf, hypothetical protein (VIMSS), at 1,959,996 to 1,960,484 yecT b1878: flhE - hypothetical protein (NCBI), at 1,960,604 to 1,960,996 flhE b1879: flhA - flagellar biosynthesis protein A (NCBI), at 1,960,996 to 1,963,074 flhA Position (kb) 1959 1960 1961Strain fitness (log2 ratio) -2 -1 0 1 2at 1959.835 kb on + strandat 1959.850 kb on + strandat 1959.850 kb on + strandat 1959.881 kb on + strandat 1959.887 kb on - strandat 1959.907 kb on + strandat 1959.932 kb on - strandat 1959.960 kb on + strandat 1959.979 kb on + strandat 1960.031 kb on + strandat 1960.035 kb on + strandat 1960.035 kb on + strandat 1960.040 kb on + strandat 1960.040 kb on + strandat 1960.065 kb on + strand, within yecTat 1960.067 kb on - strand, within yecTat 1960.079 kb on - strand, within yecTat 1960.079 kb on - strand, within yecTat 1960.120 kb on + strand, within yecTat 1960.129 kb on + strand, within yecTat 1960.144 kb on + strand, within yecTat 1960.274 kb on - strand, within yecTat 1960.274 kb on - strand, within yecTat 1960.357 kb on + strand, within yecTat 1960.358 kb on + strand, within yecTat 1960.372 kb on - strand, within yecTat 1960.390 kb on + strand, within yecTat 1960.390 kb on + strand, within yecTat 1960.391 kb on + strand, within yecTat 1960.431 kb on + strand, within yecTat 1960.431 kb on + strand, within yecTat 1960.439 kb on + strandat 1960.511 kb on - strandat 1960.511 kb on - strandat 1960.560 kb on - strandat 1960.646 kb on - strand, within flhEat 1960.680 kb on - strand, within flhEat 1960.723 kb on - strand, within flhEat 1960.723 kb on - strand, within flhEat 1960.813 kb on + strand, within flhEat 1960.844 kb on - strand, within flhEat 1960.846 kb on - strand, within flhEat 1960.846 kb on - strand, within flhEat 1960.882 kb on + strand, within flhEat 1960.882 kb on + strand, within flhEat 1960.988 kb on - strandat 1961.060 kb on - strandat 1961.060 kb on - strandat 1961.071 kb on - strandat 1961.144 kb on + strandat 1961.162 kb on - strandat 1961.264 kb on + strand, within flhAat 1961.329 kb on - strand, within flhAat 1961.415 kb on - strand, within flhAat 1961.422 kb on + strand, within flhAat 1961.456 kb on + strand, within flhA

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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1,959,835 + -0.6
1,959,850 + +0.2
1,959,850 + +0.3
1,959,881 + +0.0
1,959,887 - +0.1
1,959,907 + -0.3
1,959,932 - -1.2
1,959,960 + +0.7
1,959,979 + +0.8
1,960,031 + +0.1
1,960,035 + -1.6
1,960,035 + -0.8
1,960,040 + -0.8
1,960,040 + -0.8
1,960,065 + yecT b1877 0.14 -2.2
1,960,067 - yecT b1877 0.15 +0.4
1,960,079 - yecT b1877 0.17 +0.4
1,960,079 - yecT b1877 0.17 -1.0
1,960,120 + yecT b1877 0.25 -1.0
1,960,129 + yecT b1877 0.27 +0.5
1,960,144 + yecT b1877 0.30 +1.6
1,960,274 - yecT b1877 0.57 +0.0
1,960,274 - yecT b1877 0.57 +1.1
1,960,357 + yecT b1877 0.74 +0.4
1,960,358 + yecT b1877 0.74 -1.2
1,960,372 - yecT b1877 0.77 -0.0
1,960,390 + yecT b1877 0.81 -0.4
1,960,390 + yecT b1877 0.81 -0.8
1,960,391 + yecT b1877 0.81 -0.7
1,960,431 + yecT b1877 0.89 +2.6
1,960,431 + yecT b1877 0.89 +0.5
1,960,439 + -1.3
1,960,511 - +0.1
1,960,511 - -0.1
1,960,560 - -0.9
1,960,646 - flhE b1878 0.11 -0.1
1,960,680 - flhE b1878 0.19 -0.6
1,960,723 - flhE b1878 0.30 +0.8
1,960,723 - flhE b1878 0.30 +0.3
1,960,813 + flhE b1878 0.53 -0.2
1,960,844 - flhE b1878 0.61 -0.4
1,960,846 - flhE b1878 0.62 +0.7
1,960,846 - flhE b1878 0.62 -0.2
1,960,882 + flhE b1878 0.71 +0.6
1,960,882 + flhE b1878 0.71 +0.4
1,960,988 - -2.1
1,961,060 - -0.2
1,961,060 - -1.0
1,961,071 - +0.4
1,961,144 + -0.9
1,961,162 - -0.6
1,961,264 + flhA b1879 0.13 -0.2
1,961,329 - flhA b1879 0.16 +0.6
1,961,415 - flhA b1879 0.20 +0.3
1,961,422 + flhA b1879 0.20 -0.2
1,961,456 + flhA b1879 0.22 +0.0

Or see this region's nucleotide sequence