Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt brnQ and proY are separated by 75 nucleotides proY and malZ are separated by 158 nucleotides
b0401: brnQ - predicted branched chain amino acid transporter (LIV-II) (NCBI), at 418,815 to 420,134
brnQ
b0402: proY - predicted cryptic proline transporter (NCBI), at 420,210 to 421,583
proY
b0403: malZ - maltodextrin glucosidase (NCBI), at 421,742 to 423,556
malZ
Position (kb) 420
421
422 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 419.243 kb on + strand, within brnQ at 419.249 kb on - strand, within brnQ at 419.263 kb on + strand, within brnQ at 419.263 kb on + strand, within brnQ at 419.308 kb on + strand, within brnQ at 419.328 kb on + strand, within brnQ at 419.404 kb on + strand, within brnQ at 419.526 kb on + strand, within brnQ at 419.542 kb on + strand, within brnQ at 419.545 kb on - strand, within brnQ at 419.545 kb on - strand, within brnQ at 419.547 kb on + strand, within brnQ at 419.547 kb on + strand, within brnQ at 419.645 kb on + strand, within brnQ at 419.703 kb on + strand, within brnQ at 419.703 kb on + strand, within brnQ at 419.703 kb on + strand, within brnQ at 419.709 kb on + strand, within brnQ at 419.733 kb on + strand, within brnQ at 419.746 kb on - strand, within brnQ at 419.746 kb on - strand, within brnQ at 419.763 kb on - strand, within brnQ at 419.817 kb on - strand, within brnQ at 419.909 kb on - strand, within brnQ at 419.909 kb on - strand, within brnQ at 420.005 kb on + strand at 420.119 kb on + strand at 420.119 kb on + strand at 420.234 kb on - strand at 420.260 kb on + strand at 420.339 kb on - strand at 420.364 kb on + strand, within proY at 420.364 kb on + strand, within proY at 420.374 kb on + strand, within proY at 420.382 kb on - strand, within proY at 420.382 kb on - strand, within proY at 420.410 kb on + strand, within proY at 420.410 kb on + strand, within proY at 420.545 kb on + strand, within proY at 420.545 kb on + strand, within proY at 420.553 kb on - strand, within proY at 420.553 kb on - strand, within proY at 420.573 kb on - strand, within proY at 420.573 kb on - strand, within proY at 420.573 kb on - strand, within proY at 420.573 kb on - strand, within proY at 420.588 kb on - strand, within proY at 420.624 kb on - strand, within proY at 420.694 kb on + strand, within proY at 420.694 kb on + strand, within proY at 420.723 kb on + strand, within proY at 420.934 kb on + strand, within proY at 420.944 kb on + strand, within proY at 420.984 kb on - strand, within proY at 421.003 kb on - strand, within proY at 421.036 kb on + strand, within proY at 421.037 kb on - strand, within proY at 421.126 kb on + strand, within proY at 421.149 kb on + strand, within proY at 421.149 kb on + strand, within proY at 421.152 kb on - strand, within proY at 421.152 kb on - strand, within proY at 421.157 kb on - strand, within proY at 421.197 kb on + strand, within proY at 421.261 kb on - strand, within proY at 421.387 kb on - strand, within proY at 421.456 kb on - strand at 421.468 kb on + strand at 421.479 kb on + strand at 421.507 kb on - strand at 421.592 kb on - strand at 421.592 kb on - strand at 421.611 kb on + strand at 421.668 kb on - strand at 421.668 kb on - strand at 421.752 kb on - strand at 421.752 kb on - strand at 421.809 kb on - strand at 421.862 kb on - strand at 421.890 kb on + strand at 421.951 kb on + strand, within malZ at 421.964 kb on + strand, within malZ at 422.178 kb on - strand, within malZ at 422.220 kb on + strand, within malZ at 422.220 kb on + strand, within malZ at 422.220 kb on + strand, within malZ at 422.271 kb on - strand, within malZ at 422.291 kb on - strand, within malZ at 422.374 kb on - strand, within malZ at 422.379 kb on - strand, within malZ at 422.379 kb on - strand, within malZ at 422.494 kb on - strand, within malZ at 422.505 kb on + strand, within malZ at 422.531 kb on - strand, within malZ at 422.539 kb on + strand, within malZ
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 419,243 + brnQ b0401 0.32 +0.9 419,249 - brnQ b0401 0.33 -0.7 419,263 + brnQ b0401 0.34 -0.7 419,263 + brnQ b0401 0.34 +0.9 419,308 + brnQ b0401 0.37 +0.1 419,328 + brnQ b0401 0.39 +1.9 419,404 + brnQ b0401 0.45 -0.5 419,526 + brnQ b0401 0.54 +0.1 419,542 + brnQ b0401 0.55 +0.5 419,545 - brnQ b0401 0.55 +0.6 419,545 - brnQ b0401 0.55 -0.7 419,547 + brnQ b0401 0.55 +0.9 419,547 + brnQ b0401 0.55 +0.9 419,645 + brnQ b0401 0.63 +0.5 419,703 + brnQ b0401 0.67 -1.2 419,703 + brnQ b0401 0.67 -0.5 419,703 + brnQ b0401 0.67 +0.5 419,709 + brnQ b0401 0.68 +1.9 419,733 + brnQ b0401 0.70 +0.5 419,746 - brnQ b0401 0.71 +0.1 419,746 - brnQ b0401 0.71 +0.3 419,763 - brnQ b0401 0.72 +0.1 419,817 - brnQ b0401 0.76 -0.5 419,909 - brnQ b0401 0.83 +1.3 419,909 - brnQ b0401 0.83 -0.4 420,005 + +0.9 420,119 + -0.1 420,119 + -1.1 420,234 - -0.7 420,260 + -2.4 420,339 - -0.9 420,364 + proY b0402 0.11 -1.7 420,364 + proY b0402 0.11 -1.6 420,374 + proY b0402 0.12 -2.5 420,382 - proY b0402 0.13 +0.3 420,382 - proY b0402 0.13 -0.1 420,410 + proY b0402 0.15 -1.9 420,410 + proY b0402 0.15 -2.1 420,545 + proY b0402 0.24 -2.3 420,545 + proY b0402 0.24 -1.7 420,553 - proY b0402 0.25 +0.1 420,553 - proY b0402 0.25 -1.6 420,573 - proY b0402 0.26 -1.9 420,573 - proY b0402 0.26 -2.6 420,573 - proY b0402 0.26 -1.5 420,573 - proY b0402 0.26 -0.7 420,588 - proY b0402 0.28 -0.6 420,624 - proY b0402 0.30 -1.8 420,694 + proY b0402 0.35 -1.1 420,694 + proY b0402 0.35 +1.5 420,723 + proY b0402 0.37 -2.5 420,934 + proY b0402 0.53 -2.1 420,944 + proY b0402 0.53 -2.9 420,984 - proY b0402 0.56 -3.6 421,003 - proY b0402 0.58 -1.6 421,036 + proY b0402 0.60 -2.1 421,037 - proY b0402 0.60 -0.1 421,126 + proY b0402 0.67 -1.0 421,149 + proY b0402 0.68 -1.1 421,149 + proY b0402 0.68 -2.3 421,152 - proY b0402 0.69 -1.7 421,152 - proY b0402 0.69 +0.3 421,157 - proY b0402 0.69 -2.6 421,197 + proY b0402 0.72 -2.8 421,261 - proY b0402 0.76 -1.9 421,387 - proY b0402 0.86 -1.5 421,456 - -1.6 421,468 + -1.5 421,479 + -2.5 421,507 - -1.7 421,592 - +0.2 421,592 - +1.2 421,611 + -0.5 421,668 - -0.7 421,668 - -1.5 421,752 - -0.4 421,752 - -1.1 421,809 - +0.9 421,862 - -0.1 421,890 + +0.9 421,951 + malZ b0403 0.12 +0.6 421,964 + malZ b0403 0.12 +0.2 422,178 - malZ b0403 0.24 -0.1 422,220 + malZ b0403 0.26 -1.1 422,220 + malZ b0403 0.26 -0.1 422,220 + malZ b0403 0.26 -1.9 422,271 - malZ b0403 0.29 -0.5 422,291 - malZ b0403 0.30 +1.1 422,374 - malZ b0403 0.35 +0.1 422,379 - malZ b0403 0.35 +0.1 422,379 - malZ b0403 0.35 +0.3 422,494 - malZ b0403 0.41 +0.2 422,505 + malZ b0403 0.42 -0.1 422,531 - malZ b0403 0.43 -1.5 422,539 + malZ b0403 0.44 +0.0
Or see this region's nucleotide sequence