Experiment: CPG
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt UW163_RS08460 and UW163_RS08465 are separated by 107 nucleotides UW163_RS08465 and UW163_RS08470 are separated by 76 nucleotides UW163_RS08470 and UW163_RS08475 are separated by 44 nucleotides UW163_RS08475 and UW163_RS08480 are separated by 60 nucleotides
UW163_RS08460: UW163_RS08460 - UDP-N-acetylmuramate dehydrogenase, at 1,827,229 to 1,828,257
_RS08460
UW163_RS08465: UW163_RS08465 - YajQ family cyclic di-GMP-binding protein, at 1,828,365 to 1,828,850
_RS08465
UW163_RS08470: UW163_RS08470 - hypothetical protein, at 1,828,927 to 1,829,049
_RS08470
UW163_RS08475: UW163_RS08475 - glycerol-3-phosphate 1-O-acyltransferase PlsY, at 1,829,094 to 1,829,717
_RS08475
UW163_RS08480: UW163_RS08480 - Cys-tRNA(Pro) deacylase, at 1,829,778 to 1,830,269
_RS08480
Position (kb)
1828
1829
1830 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1828.341 kb on - strand at 1828.341 kb on - strand at 1828.385 kb on - strand at 1828.599 kb on + strand, within UW163_RS08465 at 1829.718 kb on - strand at 1829.718 kb on - strand at 1829.718 kb on - strand at 1829.749 kb on + strand at 1829.750 kb on - strand at 1829.878 kb on + strand, within UW163_RS08480 at 1829.878 kb on + strand, within UW163_RS08480 at 1829.878 kb on + strand, within UW163_RS08480 at 1829.879 kb on - strand, within UW163_RS08480 at 1829.966 kb on - strand, within UW163_RS08480
Per-strain Table
Position Strand Gene LocusTag Fraction CPG remove 1,828,341 - -0.2 1,828,341 - -3.1 1,828,385 - +0.2 1,828,599 + UW163_RS08465 0.48 -0.6 1,829,718 - +1.8 1,829,718 - -0.0 1,829,718 - -1.0 1,829,749 + -0.8 1,829,750 - +0.9 1,829,878 + UW163_RS08480 0.20 -0.0 1,829,878 + UW163_RS08480 0.20 -0.2 1,829,878 + UW163_RS08480 0.20 -0.2 1,829,879 - UW163_RS08480 0.21 -0.2 1,829,966 - UW163_RS08480 0.38 +0.9
Or see this region's nucleotide sequence