Experiment: m.b. Doxycycline hyclate 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_4399 and Echvi_4400 are separated by 17 nucleotides Echvi_4400 and Echvi_4401 are separated by 110 nucleotides
Echvi_4399: Echvi_4399 - hypothetical protein, at 5,258,617 to 5,259,918
_4399
Echvi_4400: Echvi_4400 - nucleotide sugar dehydrogenase, at 5,259,936 to 5,261,255
_4400
Echvi_4401: Echvi_4401 - Uncharacterized protein involved in exopolysaccharide biosynthesis, at 5,261,366 to 5,262,481
_4401
Position (kb)
5259
5260
5261
5262 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 5259.018 kb on + strand, within Echvi_4399 at 5259.018 kb on + strand, within Echvi_4399 at 5259.021 kb on - strand, within Echvi_4399 at 5259.023 kb on + strand, within Echvi_4399 at 5259.038 kb on + strand, within Echvi_4399 at 5259.072 kb on + strand, within Echvi_4399 at 5259.091 kb on - strand, within Echvi_4399 at 5259.310 kb on + strand, within Echvi_4399 at 5259.325 kb on + strand, within Echvi_4399 at 5259.329 kb on - strand, within Echvi_4399 at 5259.329 kb on - strand, within Echvi_4399 at 5259.330 kb on + strand, within Echvi_4399 at 5259.331 kb on - strand, within Echvi_4399 at 5259.336 kb on + strand, within Echvi_4399 at 5259.349 kb on + strand, within Echvi_4399 at 5259.350 kb on - strand, within Echvi_4399 at 5259.357 kb on - strand, within Echvi_4399 at 5259.361 kb on + strand at 5259.362 kb on - strand, within Echvi_4399 at 5259.362 kb on - strand, within Echvi_4399 at 5259.402 kb on + strand, within Echvi_4399 at 5259.529 kb on - strand, within Echvi_4399 at 5259.594 kb on + strand, within Echvi_4399 at 5259.597 kb on + strand, within Echvi_4399 at 5259.646 kb on + strand, within Echvi_4399 at 5259.793 kb on + strand at 5259.797 kb on + strand at 5259.877 kb on + strand at 5259.888 kb on + strand at 5259.895 kb on + strand at 5259.903 kb on + strand at 5259.903 kb on + strand at 5259.938 kb on - strand at 5259.952 kb on - strand at 5259.980 kb on + strand at 5259.981 kb on - strand at 5259.985 kb on + strand at 5259.985 kb on + strand at 5259.990 kb on + strand at 5259.990 kb on + strand at 5259.990 kb on + strand at 5259.991 kb on - strand at 5260.011 kb on + strand at 5260.013 kb on + strand at 5260.044 kb on - strand at 5260.077 kb on - strand, within Echvi_4400 at 5260.080 kb on + strand, within Echvi_4400 at 5260.105 kb on - strand, within Echvi_4400 at 5260.187 kb on + strand, within Echvi_4400 at 5260.187 kb on + strand, within Echvi_4400 at 5260.187 kb on + strand, within Echvi_4400 at 5260.187 kb on + strand, within Echvi_4400 at 5260.188 kb on - strand, within Echvi_4400 at 5260.189 kb on + strand, within Echvi_4400 at 5260.189 kb on + strand, within Echvi_4400 at 5260.189 kb on + strand, within Echvi_4400 at 5260.189 kb on + strand, within Echvi_4400 at 5260.189 kb on + strand, within Echvi_4400 at 5260.190 kb on - strand, within Echvi_4400 at 5260.190 kb on - strand, within Echvi_4400 at 5260.191 kb on + strand, within Echvi_4400 at 5260.191 kb on + strand, within Echvi_4400 at 5260.191 kb on + strand, within Echvi_4400 at 5260.191 kb on + strand, within Echvi_4400 at 5260.191 kb on + strand, within Echvi_4400 at 5260.191 kb on + strand, within Echvi_4400 at 5260.191 kb on + strand, within Echvi_4400 at 5260.192 kb on - strand, within Echvi_4400 at 5260.193 kb on + strand, within Echvi_4400 at 5260.193 kb on + strand, within Echvi_4400 at 5260.193 kb on + strand, within Echvi_4400 at 5260.193 kb on + strand, within Echvi_4400 at 5260.193 kb on + strand, within Echvi_4400 at 5260.193 kb on + strand, within Echvi_4400 at 5260.197 kb on + strand, within Echvi_4400 at 5260.197 kb on + strand, within Echvi_4400 at 5260.197 kb on + strand, within Echvi_4400 at 5260.197 kb on + strand, within Echvi_4400 at 5260.197 kb on + strand, within Echvi_4400 at 5260.197 kb on + strand, within Echvi_4400 at 5260.208 kb on + strand, within Echvi_4400 at 5260.208 kb on + strand, within Echvi_4400 at 5260.217 kb on + strand, within Echvi_4400 at 5260.263 kb on - strand, within Echvi_4400 at 5260.310 kb on + strand, within Echvi_4400 at 5260.311 kb on - strand, within Echvi_4400 at 5260.332 kb on - strand, within Echvi_4400 at 5260.332 kb on - strand, within Echvi_4400 at 5260.332 kb on - strand, within Echvi_4400 at 5260.335 kb on - strand, within Echvi_4400 at 5260.342 kb on - strand, within Echvi_4400 at 5260.362 kb on - strand, within Echvi_4400 at 5260.394 kb on + strand, within Echvi_4400 at 5260.406 kb on - strand, within Echvi_4400 at 5260.406 kb on - strand, within Echvi_4400 at 5260.424 kb on + strand, within Echvi_4400 at 5260.424 kb on + strand, within Echvi_4400 at 5260.425 kb on - strand, within Echvi_4400 at 5260.451 kb on + strand, within Echvi_4400 at 5260.472 kb on + strand, within Echvi_4400 at 5260.476 kb on - strand, within Echvi_4400 at 5260.508 kb on + strand, within Echvi_4400 at 5260.509 kb on - strand, within Echvi_4400 at 5260.509 kb on - strand, within Echvi_4400 at 5260.512 kb on + strand, within Echvi_4400 at 5260.578 kb on + strand, within Echvi_4400 at 5260.579 kb on - strand, within Echvi_4400 at 5260.586 kb on + strand, within Echvi_4400 at 5260.586 kb on + strand, within Echvi_4400 at 5260.587 kb on - strand, within Echvi_4400 at 5260.607 kb on + strand, within Echvi_4400 at 5260.607 kb on + strand, within Echvi_4400 at 5260.608 kb on - strand, within Echvi_4400 at 5260.608 kb on - strand, within Echvi_4400 at 5260.621 kb on + strand, within Echvi_4400 at 5260.621 kb on + strand, within Echvi_4400 at 5260.621 kb on + strand, within Echvi_4400 at 5260.622 kb on - strand, within Echvi_4400 at 5260.622 kb on - strand, within Echvi_4400 at 5260.622 kb on - strand, within Echvi_4400 at 5260.622 kb on - strand, within Echvi_4400 at 5260.625 kb on + strand, within Echvi_4400 at 5260.626 kb on - strand, within Echvi_4400 at 5260.648 kb on + strand, within Echvi_4400 at 5260.659 kb on + strand, within Echvi_4400 at 5260.659 kb on + strand, within Echvi_4400 at 5260.727 kb on + strand, within Echvi_4400 at 5260.728 kb on - strand, within Echvi_4400 at 5260.740 kb on - strand, within Echvi_4400 at 5260.765 kb on + strand, within Echvi_4400 at 5260.778 kb on + strand, within Echvi_4400 at 5260.807 kb on - strand, within Echvi_4400 at 5260.840 kb on + strand, within Echvi_4400 at 5260.847 kb on + strand, within Echvi_4400 at 5260.847 kb on + strand, within Echvi_4400 at 5260.847 kb on + strand, within Echvi_4400 at 5260.848 kb on - strand, within Echvi_4400 at 5260.848 kb on - strand, within Echvi_4400 at 5260.855 kb on + strand, within Echvi_4400 at 5260.878 kb on + strand, within Echvi_4400 at 5260.934 kb on + strand, within Echvi_4400 at 5260.934 kb on + strand, within Echvi_4400 at 5260.934 kb on + strand, within Echvi_4400 at 5260.934 kb on + strand, within Echvi_4400 at 5260.935 kb on - strand, within Echvi_4400 at 5260.935 kb on - strand, within Echvi_4400 at 5260.935 kb on - strand, within Echvi_4400 at 5260.962 kb on + strand, within Echvi_4400 at 5260.962 kb on + strand, within Echvi_4400 at 5260.968 kb on - strand, within Echvi_4400 at 5260.974 kb on - strand, within Echvi_4400 at 5261.138 kb on + strand at 5261.139 kb on - strand at 5261.139 kb on - strand at 5261.143 kb on + strand at 5261.153 kb on + strand at 5261.154 kb on - strand at 5261.154 kb on - strand at 5261.161 kb on + strand at 5261.161 kb on + strand at 5261.161 kb on + strand at 5261.161 kb on + strand at 5261.161 kb on + strand at 5261.161 kb on + strand at 5261.162 kb on - strand at 5261.162 kb on - strand at 5261.162 kb on - strand at 5261.162 kb on - strand at 5261.198 kb on - strand at 5261.198 kb on - strand at 5261.348 kb on - strand at 5261.398 kb on + strand at 5261.398 kb on + strand at 5261.398 kb on + strand at 5261.408 kb on + strand at 5261.409 kb on - strand at 5261.414 kb on + strand at 5261.417 kb on + strand at 5261.417 kb on + strand at 5261.417 kb on + strand at 5261.420 kb on + strand at 5261.435 kb on + strand at 5261.436 kb on - strand at 5261.463 kb on - strand at 5261.465 kb on + strand at 5261.465 kb on + strand at 5261.466 kb on - strand at 5261.466 kb on - strand at 5261.479 kb on + strand, within Echvi_4401 at 5261.507 kb on - strand, within Echvi_4401 at 5261.509 kb on + strand, within Echvi_4401 at 5261.510 kb on - strand, within Echvi_4401 at 5261.510 kb on - strand, within Echvi_4401 at 5261.536 kb on + strand, within Echvi_4401 at 5261.536 kb on + strand, within Echvi_4401 at 5261.536 kb on + strand, within Echvi_4401 at 5261.537 kb on - strand, within Echvi_4401 at 5261.557 kb on + strand, within Echvi_4401 at 5261.639 kb on + strand, within Echvi_4401 at 5261.672 kb on - strand, within Echvi_4401 at 5261.700 kb on - strand, within Echvi_4401 at 5261.743 kb on + strand, within Echvi_4401 at 5261.802 kb on - strand, within Echvi_4401 at 5261.881 kb on + strand, within Echvi_4401 at 5261.950 kb on + strand, within Echvi_4401 at 5261.968 kb on + strand, within Echvi_4401 at 5261.972 kb on + strand, within Echvi_4401 at 5261.973 kb on - strand, within Echvi_4401 at 5261.973 kb on - strand, within Echvi_4401 at 5262.012 kb on - strand, within Echvi_4401 at 5262.012 kb on - strand, within Echvi_4401 at 5262.039 kb on + strand, within Echvi_4401 at 5262.050 kb on - strand, within Echvi_4401 at 5262.076 kb on - strand, within Echvi_4401 at 5262.084 kb on + strand, within Echvi_4401 at 5262.084 kb on + strand, within Echvi_4401 at 5262.087 kb on + strand, within Echvi_4401 at 5262.088 kb on - strand, within Echvi_4401 at 5262.088 kb on - strand, within Echvi_4401 at 5262.088 kb on - strand, within Echvi_4401 at 5262.097 kb on - strand, within Echvi_4401 at 5262.140 kb on + strand, within Echvi_4401 at 5262.141 kb on - strand, within Echvi_4401 at 5262.163 kb on + strand, within Echvi_4401 at 5262.163 kb on + strand, within Echvi_4401 at 5262.163 kb on + strand, within Echvi_4401 at 5262.163 kb on + strand, within Echvi_4401 at 5262.185 kb on + strand, within Echvi_4401 at 5262.185 kb on + strand, within Echvi_4401 at 5262.185 kb on + strand, within Echvi_4401 at 5262.185 kb on + strand, within Echvi_4401 at 5262.186 kb on - strand, within Echvi_4401 at 5262.186 kb on - strand, within Echvi_4401 at 5262.186 kb on - strand, within Echvi_4401
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Doxycycline hyclate 0.032 mg/ml remove 5,259,018 + Echvi_4399 0.31 +3.0 5,259,018 + Echvi_4399 0.31 +2.5 5,259,021 - Echvi_4399 0.31 +0.7 5,259,023 + Echvi_4399 0.31 +2.9 5,259,038 + Echvi_4399 0.32 +3.9 5,259,072 + Echvi_4399 0.35 +2.1 5,259,091 - Echvi_4399 0.36 +1.5 5,259,310 + Echvi_4399 0.53 +2.4 5,259,325 + Echvi_4399 0.54 +2.6 5,259,329 - Echvi_4399 0.55 +1.8 5,259,329 - Echvi_4399 0.55 +2.6 5,259,330 + Echvi_4399 0.55 +3.6 5,259,331 - Echvi_4399 0.55 -0.0 5,259,336 + Echvi_4399 0.55 +4.4 5,259,349 + Echvi_4399 0.56 +2.7 5,259,350 - Echvi_4399 0.56 +4.6 5,259,357 - Echvi_4399 0.57 +4.4 5,259,361 + +4.6 5,259,362 - Echvi_4399 0.57 +2.5 5,259,362 - Echvi_4399 0.57 +2.1 5,259,402 + Echvi_4399 0.60 +1.7 5,259,529 - Echvi_4399 0.70 +1.5 5,259,594 + Echvi_4399 0.75 +3.0 5,259,597 + Echvi_4399 0.75 +2.8 5,259,646 + Echvi_4399 0.79 +2.7 5,259,793 + +2.5 5,259,797 + +2.1 5,259,877 + +2.9 5,259,888 + +3.1 5,259,895 + +2.6 5,259,903 + +1.9 5,259,903 + +1.7 5,259,938 - +1.2 5,259,952 - +0.2 5,259,980 + +1.0 5,259,981 - -2.8 5,259,985 + +0.3 5,259,985 + +0.1 5,259,990 + +2.3 5,259,990 + +1.7 5,259,990 + +1.3 5,259,991 - +1.0 5,260,011 + +2.0 5,260,013 + +2.0 5,260,044 - -0.2 5,260,077 - Echvi_4400 0.11 +0.5 5,260,080 + Echvi_4400 0.11 +1.4 5,260,105 - Echvi_4400 0.13 +1.3 5,260,187 + Echvi_4400 0.19 +0.9 5,260,187 + Echvi_4400 0.19 +0.8 5,260,187 + Echvi_4400 0.19 +1.0 5,260,187 + Echvi_4400 0.19 +3.0 5,260,188 - Echvi_4400 0.19 -0.1 5,260,189 + Echvi_4400 0.19 +1.9 5,260,189 + Echvi_4400 0.19 +1.8 5,260,189 + Echvi_4400 0.19 +0.6 5,260,189 + Echvi_4400 0.19 +0.3 5,260,189 + Echvi_4400 0.19 +1.0 5,260,190 - Echvi_4400 0.19 +1.8 5,260,190 - Echvi_4400 0.19 +0.4 5,260,191 + Echvi_4400 0.19 +1.2 5,260,191 + Echvi_4400 0.19 +0.7 5,260,191 + Echvi_4400 0.19 +0.7 5,260,191 + Echvi_4400 0.19 +0.1 5,260,191 + Echvi_4400 0.19 +0.2 5,260,191 + Echvi_4400 0.19 +0.1 5,260,191 + Echvi_4400 0.19 +0.4 5,260,192 - Echvi_4400 0.19 +1.3 5,260,193 + Echvi_4400 0.19 +0.9 5,260,193 + Echvi_4400 0.19 +1.4 5,260,193 + Echvi_4400 0.19 +1.2 5,260,193 + Echvi_4400 0.19 +1.5 5,260,193 + Echvi_4400 0.19 +2.2 5,260,193 + Echvi_4400 0.19 +1.7 5,260,197 + Echvi_4400 0.20 +0.6 5,260,197 + Echvi_4400 0.20 +1.1 5,260,197 + Echvi_4400 0.20 -1.0 5,260,197 + Echvi_4400 0.20 +1.6 5,260,197 + Echvi_4400 0.20 +1.8 5,260,197 + Echvi_4400 0.20 +0.5 5,260,208 + Echvi_4400 0.21 +2.3 5,260,208 + Echvi_4400 0.21 +2.2 5,260,217 + Echvi_4400 0.21 -0.0 5,260,263 - Echvi_4400 0.25 +1.1 5,260,310 + Echvi_4400 0.28 +1.6 5,260,311 - Echvi_4400 0.28 +1.4 5,260,332 - Echvi_4400 0.30 -0.0 5,260,332 - Echvi_4400 0.30 +2.0 5,260,332 - Echvi_4400 0.30 +2.0 5,260,335 - Echvi_4400 0.30 -0.9 5,260,342 - Echvi_4400 0.31 +1.5 5,260,362 - Echvi_4400 0.32 +0.3 5,260,394 + Echvi_4400 0.35 +1.6 5,260,406 - Echvi_4400 0.36 +0.5 5,260,406 - Echvi_4400 0.36 +1.3 5,260,424 + Echvi_4400 0.37 +2.7 5,260,424 + Echvi_4400 0.37 +1.6 5,260,425 - Echvi_4400 0.37 -0.6 5,260,451 + Echvi_4400 0.39 +1.8 5,260,472 + Echvi_4400 0.41 +2.6 5,260,476 - Echvi_4400 0.41 +0.6 5,260,508 + Echvi_4400 0.43 +1.9 5,260,509 - Echvi_4400 0.43 +0.1 5,260,509 - Echvi_4400 0.43 +0.5 5,260,512 + Echvi_4400 0.44 -0.4 5,260,578 + Echvi_4400 0.49 +0.8 5,260,579 - Echvi_4400 0.49 -0.1 5,260,586 + Echvi_4400 0.49 +0.9 5,260,586 + Echvi_4400 0.49 +1.3 5,260,587 - Echvi_4400 0.49 -0.1 5,260,607 + Echvi_4400 0.51 +1.7 5,260,607 + Echvi_4400 0.51 +1.5 5,260,608 - Echvi_4400 0.51 +0.6 5,260,608 - Echvi_4400 0.51 -1.5 5,260,621 + Echvi_4400 0.52 +1.5 5,260,621 + Echvi_4400 0.52 +0.9 5,260,621 + Echvi_4400 0.52 +1.0 5,260,622 - Echvi_4400 0.52 -0.1 5,260,622 - Echvi_4400 0.52 +1.6 5,260,622 - Echvi_4400 0.52 -0.0 5,260,622 - Echvi_4400 0.52 -0.1 5,260,625 + Echvi_4400 0.52 +1.6 5,260,626 - Echvi_4400 0.52 +3.7 5,260,648 + Echvi_4400 0.54 +1.5 5,260,659 + Echvi_4400 0.55 +1.2 5,260,659 + Echvi_4400 0.55 +0.9 5,260,727 + Echvi_4400 0.60 +1.3 5,260,728 - Echvi_4400 0.60 -1.0 5,260,740 - Echvi_4400 0.61 -1.1 5,260,765 + Echvi_4400 0.63 +1.5 5,260,778 + Echvi_4400 0.64 +2.0 5,260,807 - Echvi_4400 0.66 +0.2 5,260,840 + Echvi_4400 0.68 +3.3 5,260,847 + Echvi_4400 0.69 +1.1 5,260,847 + Echvi_4400 0.69 +1.7 5,260,847 + Echvi_4400 0.69 +1.7 5,260,848 - Echvi_4400 0.69 -1.4 5,260,848 - Echvi_4400 0.69 -0.6 5,260,855 + Echvi_4400 0.70 +2.7 5,260,878 + Echvi_4400 0.71 +0.5 5,260,934 + Echvi_4400 0.76 +1.7 5,260,934 + Echvi_4400 0.76 +0.6 5,260,934 + Echvi_4400 0.76 +1.8 5,260,934 + Echvi_4400 0.76 +2.8 5,260,935 - Echvi_4400 0.76 -0.2 5,260,935 - Echvi_4400 0.76 +0.9 5,260,935 - Echvi_4400 0.76 -0.1 5,260,962 + Echvi_4400 0.78 +0.7 5,260,962 + Echvi_4400 0.78 +0.8 5,260,968 - Echvi_4400 0.78 +0.4 5,260,974 - Echvi_4400 0.79 -0.6 5,261,138 + +1.7 5,261,139 - -1.7 5,261,139 - -0.2 5,261,143 + +0.5 5,261,153 + +1.6 5,261,154 - +1.4 5,261,154 - +0.4 5,261,161 + +1.8 5,261,161 + +1.9 5,261,161 + +2.1 5,261,161 + +1.3 5,261,161 + +2.7 5,261,161 + +2.9 5,261,162 - +1.1 5,261,162 - +2.0 5,261,162 - +0.9 5,261,162 - +1.0 5,261,198 - +4.3 5,261,198 - +0.8 5,261,348 - +0.6 5,261,398 + +2.7 5,261,398 + +1.7 5,261,398 + +1.7 5,261,408 + +2.9 5,261,409 - -1.1 5,261,414 + +3.1 5,261,417 + +1.9 5,261,417 + +3.4 5,261,417 + +5.0 5,261,420 + +2.2 5,261,435 + +3.6 5,261,436 - +1.7 5,261,463 - +0.6 5,261,465 + +2.5 5,261,465 + +4.2 5,261,466 - +1.6 5,261,466 - +0.2 5,261,479 + Echvi_4401 0.10 +2.4 5,261,507 - Echvi_4401 0.13 +1.1 5,261,509 + Echvi_4401 0.13 +2.6 5,261,510 - Echvi_4401 0.13 +1.4 5,261,510 - Echvi_4401 0.13 +1.1 5,261,536 + Echvi_4401 0.15 +3.0 5,261,536 + Echvi_4401 0.15 +4.2 5,261,536 + Echvi_4401 0.15 +4.8 5,261,537 - Echvi_4401 0.15 +0.4 5,261,557 + Echvi_4401 0.17 +4.0 5,261,639 + Echvi_4401 0.24 +3.0 5,261,672 - Echvi_4401 0.27 +2.0 5,261,700 - Echvi_4401 0.30 +2.0 5,261,743 + Echvi_4401 0.34 +3.2 5,261,802 - Echvi_4401 0.39 +0.9 5,261,881 + Echvi_4401 0.46 +3.4 5,261,950 + Echvi_4401 0.52 +3.1 5,261,968 + Echvi_4401 0.54 +3.2 5,261,972 + Echvi_4401 0.54 +1.2 5,261,973 - Echvi_4401 0.54 +2.7 5,261,973 - Echvi_4401 0.54 +1.3 5,262,012 - Echvi_4401 0.58 +1.0 5,262,012 - Echvi_4401 0.58 +1.6 5,262,039 + Echvi_4401 0.60 +2.5 5,262,050 - Echvi_4401 0.61 +0.5 5,262,076 - Echvi_4401 0.64 +1.3 5,262,084 + Echvi_4401 0.64 +4.3 5,262,084 + Echvi_4401 0.64 +3.1 5,262,087 + Echvi_4401 0.65 +2.1 5,262,088 - Echvi_4401 0.65 +1.7 5,262,088 - Echvi_4401 0.65 +0.2 5,262,088 - Echvi_4401 0.65 +0.3 5,262,097 - Echvi_4401 0.66 +1.3 5,262,140 + Echvi_4401 0.69 +2.6 5,262,141 - Echvi_4401 0.69 +0.7 5,262,163 + Echvi_4401 0.71 +2.8 5,262,163 + Echvi_4401 0.71 +3.1 5,262,163 + Echvi_4401 0.71 +2.9 5,262,163 + Echvi_4401 0.71 +3.0 5,262,185 + Echvi_4401 0.73 +2.3 5,262,185 + Echvi_4401 0.73 +3.0 5,262,185 + Echvi_4401 0.73 +3.6 5,262,185 + Echvi_4401 0.73 +3.2 5,262,186 - Echvi_4401 0.73 +0.9 5,262,186 - Echvi_4401 0.73 +0.1 5,262,186 - Echvi_4401 0.73 +0.6
Or see this region's nucleotide sequence