Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt perR and insN are separated by 95 nucleotides insN and insI-1 are separated by 67 nucleotides
b0254: perR - CP4-6 prophage; predicted DNA-binding transcriptional regulator (NCBI), at 268,513 to 269,406
perR
b4587: insN - no description, at 269,502 to 269,759
insN
b0256: insI-1 - CP4-6 prophage; IS30 transposase (NCBI), at 269,827 to 270,978
insI-1
Position (kb) 269
270 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 268.538 kb on - strand at 268.584 kb on - strand at 268.591 kb on - strand at 268.591 kb on - strand at 268.623 kb on - strand, within perR at 268.645 kb on - strand, within perR at 268.657 kb on - strand, within perR at 268.809 kb on - strand, within perR at 268.853 kb on + strand, within perR at 268.873 kb on + strand, within perR at 268.873 kb on + strand, within perR at 268.928 kb on + strand, within perR at 268.967 kb on - strand, within perR at 268.967 kb on - strand, within perR at 268.971 kb on - strand, within perR at 268.971 kb on - strand, within perR at 268.993 kb on - strand, within perR at 269.108 kb on + strand, within perR at 269.108 kb on + strand, within perR at 269.116 kb on - strand, within perR at 269.116 kb on - strand, within perR at 269.116 kb on - strand, within perR at 269.131 kb on + strand, within perR at 269.157 kb on - strand, within perR at 269.199 kb on + strand, within perR at 269.199 kb on + strand, within perR at 269.302 kb on + strand, within perR at 269.458 kb on + strand at 269.468 kb on + strand at 269.468 kb on + strand at 269.486 kb on - strand at 269.528 kb on + strand, within insN at 269.536 kb on - strand, within insN at 269.564 kb on + strand, within insN at 269.564 kb on + strand, within insN at 269.564 kb on + strand, within insN at 269.564 kb on + strand, within insN at 269.572 kb on - strand, within insN at 269.572 kb on - strand, within insN at 269.596 kb on - strand, within insN at 269.596 kb on - strand, within insN at 269.598 kb on + strand, within insN
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 268,538 - -0.2 268,584 - +0.4 268,591 - -0.2 268,591 - +1.0 268,623 - perR b0254 0.12 -0.6 268,645 - perR b0254 0.15 +0.7 268,657 - perR b0254 0.16 +0.7 268,809 - perR b0254 0.33 +1.2 268,853 + perR b0254 0.38 -2.2 268,873 + perR b0254 0.40 +0.2 268,873 + perR b0254 0.40 +0.5 268,928 + perR b0254 0.46 -0.0 268,967 - perR b0254 0.51 -0.6 268,967 - perR b0254 0.51 +1.0 268,971 - perR b0254 0.51 +2.0 268,971 - perR b0254 0.51 +0.7 268,993 - perR b0254 0.54 -0.2 269,108 + perR b0254 0.67 -0.1 269,108 + perR b0254 0.67 -2.0 269,116 - perR b0254 0.67 +0.8 269,116 - perR b0254 0.67 +2.8 269,116 - perR b0254 0.67 +4.7 269,131 + perR b0254 0.69 +0.4 269,157 - perR b0254 0.72 +2.0 269,199 + perR b0254 0.77 +3.2 269,199 + perR b0254 0.77 -0.4 269,302 + perR b0254 0.88 -0.6 269,458 + +0.0 269,468 + +1.5 269,468 + +1.4 269,486 - -2.0 269,528 + insN b4587 0.10 -1.5 269,536 - insN b4587 0.13 +0.0 269,564 + insN b4587 0.24 -1.0 269,564 + insN b4587 0.24 -0.8 269,564 + insN b4587 0.24 -0.4 269,564 + insN b4587 0.24 +0.4 269,572 - insN b4587 0.27 -0.2 269,572 - insN b4587 0.27 -1.0 269,596 - insN b4587 0.36 -0.6 269,596 - insN b4587 0.36 -0.6 269,598 + insN b4587 0.37 -0.3
Or see this region's nucleotide sequence