Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ykfB and yafY are separated by 22 nucleotides yafY and ykfL overlap by 1 nucleotides ykfL and ykfK are separated by 1 nucleotides ykfK and yafZ are separated by 216 nucleotides
b0250: ykfB - CP4-6 prophage; predicted protein (NCBI), at 264,844 to 265,311
ykfB
b0251: yafY - CP4-6 prophage; predicted DNA-binding transcriptional regulator (RefSeq), at 265,334 to 265,777
yafY
b4627: ykfL - no description, at 265,777 to 265,998
ykfL
b4628: ykfK - no description, at 266,000 to 266,191
ykfK
b0252: yafZ - orf, hypothetical protein (VIMSS), at 266,408 to 267,229
yafZ
Position (kb) 265
266 Strain fitness (log2 ratio) -1
0
1
2 at 264.953 kb on + strand, within ykfB at 265.019 kb on - strand, within ykfB at 265.140 kb on - strand, within ykfB at 265.234 kb on - strand, within ykfB at 265.234 kb on - strand, within ykfB at 265.275 kb on - strand at 265.362 kb on + strand at 265.426 kb on - strand, within yafY at 265.436 kb on - strand, within yafY at 265.519 kb on - strand, within yafY at 265.903 kb on + strand, within ykfL at 265.906 kb on - strand, within ykfL at 265.943 kb on - strand, within ykfL at 265.943 kb on - strand, within ykfL at 265.945 kb on + strand, within ykfL at 265.945 kb on + strand, within ykfL at 265.951 kb on - strand, within ykfL at 266.018 kb on + strand at 266.018 kb on + strand at 266.187 kb on + strand at 266.217 kb on - strand at 266.258 kb on + strand at 266.267 kb on - strand at 266.267 kb on - strand at 266.267 kb on - strand at 266.353 kb on - strand at 266.433 kb on + strand at 266.436 kb on - strand at 266.449 kb on + strand at 266.464 kb on - strand at 266.569 kb on - strand, within yafZ at 266.668 kb on - strand, within yafZ at 266.773 kb on - strand, within yafZ at 266.810 kb on - strand, within yafZ at 266.946 kb on - strand, within yafZ
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 264,953 + ykfB b0250 0.23 -0.5 265,019 - ykfB b0250 0.37 +0.4 265,140 - ykfB b0250 0.63 -0.4 265,234 - ykfB b0250 0.83 -0.8 265,234 - ykfB b0250 0.83 +1.4 265,275 - -0.1 265,362 + -0.2 265,426 - yafY b0251 0.21 +0.4 265,436 - yafY b0251 0.23 +2.7 265,519 - yafY b0251 0.42 +0.0 265,903 + ykfL b4627 0.57 -0.6 265,906 - ykfL b4627 0.58 +1.0 265,943 - ykfL b4627 0.75 -1.6 265,943 - ykfL b4627 0.75 -1.0 265,945 + ykfL b4627 0.76 +2.0 265,945 + ykfL b4627 0.76 +1.4 265,951 - ykfL b4627 0.78 -1.6 266,018 + +1.4 266,018 + +0.8 266,187 + +0.6 266,217 - -0.0 266,258 + -0.5 266,267 - -0.8 266,267 - +1.1 266,267 - +2.0 266,353 - +0.7 266,433 + -0.4 266,436 - +1.4 266,449 + +0.5 266,464 - +0.8 266,569 - yafZ b0252 0.20 -0.1 266,668 - yafZ b0252 0.32 -0.2 266,773 - yafZ b0252 0.44 -0.6 266,810 - yafZ b0252 0.49 -0.2 266,946 - yafZ b0252 0.65 -0.9
Or see this region's nucleotide sequence