Strain Fitness in Escherichia coli BW25113 around b3393

Experiment: Doxycycline hyclate 0.001 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Doxycycline hyclate 0.001 mg/ml
3,518,047	+	hofQ	b3391	0.45	+0.1
3,518,136	-	hofQ	b3391	0.52	+0.8
3,518,136	-	hofQ	b3391	0.52	-0.8
3,518,169	-	hofQ	b3391	0.55	-1.2
3,518,180	-	hofQ	b3391	0.56	+0.9
3,518,211	+	hofQ	b3391	0.58	-1.1
3,518,221	+	hofQ	b3391	0.59	+0.8
3,518,221	+	hofQ	b3391	0.59	-0.2
3,518,294	+	hofQ	b3391	0.65	-0.7
3,518,294	-	hofQ	b3391	0.65	-0.7
3,518,294	-	hofQ	b3391	0.65	-0.1
3,518,304	-	hofQ	b3391	0.66	-0.1
3,518,314	-	hofQ	b3391	0.67	-0.2
3,518,358	+	hofQ	b3391	0.70	+0.1
3,518,358	+	hofQ	b3391	0.70	-0.0
3,518,457	+	hofQ	b3391	0.78	-0.2
3,518,463	-	hofQ	b3391	0.79	+1.2
3,518,463	-	hofQ	b3391	0.79	-1.5
3,518,465	-	hofQ	b3391	0.79	+0.2
3,518,465	-	hofQ	b3391	0.79	-1.1
3,518,465	-	hofQ	b3391	0.79	-0.6
3,518,466	-	hofQ	b3391	0.79	-0.4
3,518,481	-	hofQ	b3391	0.80	-0.8
3,518,488	+	hofQ	b3391	0.81	+0.8
3,518,538	+	hofQ	b3391	0.85	-0.2
3,518,543	+	hofQ	b3391	0.85	+0.5
3,518,546	+	hofQ	b3391	0.85	-0.5
3,518,593	-	hofQ	b3391	0.89	-0.8
3,518,593	-	hofQ	b3391	0.89	-0.3
3,518,692	-				-0.9
3,518,692	-				+0.7
3,518,709	-				-1.5
3,518,740	-	yrfA	b3392	0.25	-1.8
3,518,740	-	yrfA	b3392	0.25	+0.5
3,518,751	-	yrfA	b3392	0.28	+0.5
3,518,776	+	yrfA	b3392	0.34	+0.1
3,518,786	+	yrfA	b3392	0.37	+0.8
3,518,786	+	yrfA	b3392	0.37	-0.7
3,518,794	-	yrfA	b3392	0.39	-0.6
3,518,794	-	yrfA	b3392	0.39	-0.4
3,518,841	+	yrfA	b3392	0.50	+1.5
3,518,852	-	yrfA	b3392	0.53	-0.8
3,518,883	-	yrfA	b3392	0.61	+0.5
3,518,883	-	yrfA	b3392	0.61	+0.5
3,518,895	-	yrfA	b3392	0.64	-1.0
3,518,895	-	yrfA	b3392	0.64	+0.2
3,518,915	+	yrfA	b3392	0.69	+0.5
3,518,981	+	yrfA	b3392	0.85	+0.5
3,518,989	-	yrfA	b3392	0.87	+0.7
3,519,000	+	yrfA	b3392	0.90	-1.3
3,519,013	-				+2.1
3,519,013	-				+0.9
3,519,013	-				+0.2
3,519,027	+				+0.0
3,519,027	+				+1.2
3,519,027	+				-0.8
3,519,113	+	yrfB	b3393	0.19	-0.9
3,519,123	+	yrfB	b3393	0.21	-0.2
3,519,123	+	yrfB	b3393	0.21	-1.8
3,519,150	+	yrfB	b3393	0.27	+0.5
3,519,189	+	yrfB	b3393	0.36	-1.5
3,519,189	+	yrfB	b3393	0.36	-2.7
3,519,290	+	yrfB	b3393	0.59	+0.2
3,519,292	+	yrfB	b3393	0.59	+1.5
3,519,363	-	yrfB	b3393	0.75	-2.3
3,519,429	+				+0.5
3,519,471	+				+0.4
3,519,471	+				+0.0
3,519,483	+				+0.5
3,519,509	+	yrfC	b3394	0.10	+0.7
3,519,509	+	yrfC	b3394	0.10	+0.4
3,519,529	+	yrfC	b3394	0.14	-0.5
3,519,556	-	yrfC	b3394	0.19	-0.2
3,519,556	-	yrfC	b3394	0.19	-0.1
3,519,608	-	yrfC	b3394	0.28	-1.0
3,519,609	+	yrfC	b3394	0.29	-1.1
3,519,609	+	yrfC	b3394	0.29	+1.5
3,519,641	-	yrfC	b3394	0.34	+1.3
3,519,731	-	yrfC	b3394	0.51	-0.2
3,519,877	+	yrfC	b3394	0.78	-0.6
3,519,885	-	yrfC	b3394	0.80	-2.3
3,519,971	-				-0.3
3,519,995	+				-0.1
3,520,003	-				-0.7
3,520,079	+	yrfD	b3395	0.11	+0.1
3,520,098	-	yrfD	b3395	0.13	+0.5
3,520,153	-	yrfD	b3395	0.20	-0.7
3,520,163	-	yrfD	b3395	0.22	+0.2
3,520,193	+	yrfD	b3395	0.26	+0.6
3,520,193	+	yrfD	b3395	0.26	-0.8
3,520,201	-	yrfD	b3395	0.27	+0.8
3,520,215	+	yrfD	b3395	0.28	-1.3
3,520,215	+	yrfD	b3395	0.28	+0.5
3,520,276	+	yrfD	b3395	0.36	+1.5
3,520,279	+	yrfD	b3395	0.37	-0.8
3,520,289	-	yrfD	b3395	0.38	+0.2
3,520,357	-	yrfD	b3395	0.47	+0.5
3,520,357	-	yrfD	b3395	0.47	-0.5
3,520,388	-	yrfD	b3395	0.51	+0.0
3,520,409	-	yrfD	b3395	0.53	+0.5
3,520,427	+	yrfD	b3395	0.56	+2.1
3,520,434	+				+0.5
3,520,436	+	yrfD	b3395	0.57	-0.5
3,520,436	+	yrfD	b3395	0.57	+0.3
3,520,437	-	yrfD	b3395	0.57	-0.9
3,520,468	-	yrfD	b3395	0.61	-1.1