Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhdE and mreD are separated by 8 nucleotides mreD and mreC overlap by 1 nucleotides mreC and mreB are separated by 65 nucleotides
b3248: yhdE - Maf-like protein (NCBI), at 3,395,807 to 3,396,400
yhdE
b3249: mreD - cell wall structural complex MreBCD transmembrane component MreD (NCBI), at 3,396,409 to 3,396,897
mreD
b3250: mreC - cell wall structural complex MreBCD transmembrane component MreC (NCBI), at 3,396,897 to 3,398,000
mreC
b3251: mreB - regulator of ftsI, penicillin binding protein 3, septation function (VIMSS), at 3,398,066 to 3,399,109
mreB
Position (kb)
3396
3397
3398
3399 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3395.897 kb on - strand, within yhdE at 3395.897 kb on - strand, within yhdE at 3395.923 kb on + strand, within yhdE at 3395.923 kb on + strand, within yhdE at 3395.941 kb on + strand, within yhdE at 3395.956 kb on - strand, within yhdE at 3395.964 kb on - strand, within yhdE at 3395.966 kb on + strand, within yhdE at 3395.987 kb on - strand, within yhdE at 3395.987 kb on - strand, within yhdE at 3396.058 kb on - strand, within yhdE at 3396.117 kb on - strand, within yhdE at 3396.139 kb on + strand, within yhdE at 3396.166 kb on - strand, within yhdE at 3396.166 kb on - strand, within yhdE at 3396.176 kb on - strand, within yhdE at 3396.186 kb on - strand, within yhdE at 3396.192 kb on + strand, within yhdE at 3396.231 kb on + strand, within yhdE at 3396.231 kb on + strand, within yhdE at 3396.256 kb on + strand, within yhdE at 3396.267 kb on + strand, within yhdE at 3396.269 kb on + strand, within yhdE at 3396.275 kb on - strand, within yhdE at 3396.275 kb on - strand, within yhdE at 3396.287 kb on - strand, within yhdE at 3396.287 kb on - strand, within yhdE at 3396.307 kb on - strand, within yhdE at 3396.317 kb on - strand, within yhdE at 3396.333 kb on - strand, within yhdE at 3396.344 kb on - strand at 3396.344 kb on - strand at 3396.364 kb on - strand at 3396.364 kb on - strand at 3396.417 kb on - strand at 3396.924 kb on - strand at 3396.924 kb on - strand at 3396.934 kb on - strand at 3396.962 kb on - strand at 3397.084 kb on - strand, within mreC at 3397.174 kb on - strand, within mreC at 3397.973 kb on - strand at 3397.974 kb on - strand at 3397.991 kb on - strand at 3398.003 kb on - strand at 3398.031 kb on - strand at 3398.032 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 3,395,897 - yhdE b3248 0.15 -1.1 3,395,897 - yhdE b3248 0.15 -0.5 3,395,923 + yhdE b3248 0.20 +0.7 3,395,923 + yhdE b3248 0.20 +0.0 3,395,941 + yhdE b3248 0.23 +0.8 3,395,956 - yhdE b3248 0.25 -1.5 3,395,964 - yhdE b3248 0.26 +0.7 3,395,966 + yhdE b3248 0.27 +0.5 3,395,987 - yhdE b3248 0.30 -0.3 3,395,987 - yhdE b3248 0.30 -0.6 3,396,058 - yhdE b3248 0.42 -2.3 3,396,117 - yhdE b3248 0.52 +0.5 3,396,139 + yhdE b3248 0.56 +0.8 3,396,166 - yhdE b3248 0.60 -0.5 3,396,166 - yhdE b3248 0.60 -2.3 3,396,176 - yhdE b3248 0.62 +1.1 3,396,186 - yhdE b3248 0.64 -0.9 3,396,192 + yhdE b3248 0.65 -0.2 3,396,231 + yhdE b3248 0.71 -0.1 3,396,231 + yhdE b3248 0.71 +1.9 3,396,256 + yhdE b3248 0.76 +1.1 3,396,267 + yhdE b3248 0.77 +0.9 3,396,269 + yhdE b3248 0.78 -0.5 3,396,275 - yhdE b3248 0.79 -1.1 3,396,275 - yhdE b3248 0.79 +0.9 3,396,287 - yhdE b3248 0.81 -0.5 3,396,287 - yhdE b3248 0.81 +0.5 3,396,307 - yhdE b3248 0.84 +0.1 3,396,317 - yhdE b3248 0.86 -0.3 3,396,333 - yhdE b3248 0.89 +1.9 3,396,344 - -0.5 3,396,344 - +0.5 3,396,364 - +0.5 3,396,364 - -1.5 3,396,417 - -0.8 3,396,924 - +0.7 3,396,924 - +0.8 3,396,934 - +0.5 3,396,962 - +0.5 3,397,084 - mreC b3250 0.17 +0.5 3,397,174 - mreC b3250 0.25 +3.9 3,397,973 - +1.6 3,397,974 - +0.8 3,397,991 - -0.5 3,398,003 - -1.1 3,398,031 - +0.9 3,398,032 - -0.1
Or see this region's nucleotide sequence