Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhcG and yhcH are separated by 59 nucleotides yhcH and yhcI overlap by 4 nucleotides yhcI and nanE overlap by 4 nucleotides
b3220: yhcG - hypothetical protein (NCBI), at 3,365,849 to 3,366,976
yhcG
b3221: yhcH - hypothetical protein (NCBI), at 3,367,036 to 3,367,500
yhcH
b3222: yhcI - putative NAGC-like transcriptional regulator (VIMSS), at 3,367,497 to 3,368,372
yhcI
b3223: nanE - predicted N-acetylmannosamine-6-P epimerase (NCBI), at 3,368,369 to 3,369,058
nanE
Position (kb)
3367
3368 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3366.036 kb on - strand, within yhcG at 3366.036 kb on - strand, within yhcG at 3366.046 kb on + strand, within yhcG at 3366.055 kb on + strand, within yhcG at 3366.055 kb on + strand, within yhcG at 3366.079 kb on + strand, within yhcG at 3366.091 kb on + strand, within yhcG at 3366.091 kb on + strand, within yhcG at 3366.184 kb on + strand, within yhcG at 3366.275 kb on + strand, within yhcG at 3366.275 kb on + strand, within yhcG at 3366.355 kb on - strand, within yhcG at 3366.355 kb on - strand, within yhcG at 3366.359 kb on - strand, within yhcG at 3366.399 kb on + strand, within yhcG at 3366.404 kb on + strand, within yhcG at 3366.404 kb on + strand, within yhcG at 3366.409 kb on - strand, within yhcG at 3366.446 kb on + strand, within yhcG at 3366.484 kb on - strand, within yhcG at 3366.484 kb on - strand, within yhcG at 3366.683 kb on - strand, within yhcG at 3366.683 kb on - strand, within yhcG at 3366.684 kb on + strand, within yhcG at 3366.684 kb on + strand, within yhcG at 3366.692 kb on - strand, within yhcG at 3366.720 kb on + strand, within yhcG at 3366.760 kb on + strand, within yhcG at 3366.760 kb on + strand, within yhcG at 3366.808 kb on + strand, within yhcG at 3366.856 kb on - strand, within yhcG at 3366.857 kb on + strand, within yhcG at 3366.906 kb on + strand at 3366.924 kb on - strand at 3366.955 kb on - strand at 3366.960 kb on + strand at 3366.960 kb on + strand at 3366.960 kb on + strand at 3366.967 kb on - strand at 3366.968 kb on - strand at 3367.219 kb on - strand, within yhcH at 3367.221 kb on + strand, within yhcH at 3367.238 kb on + strand, within yhcH at 3367.246 kb on - strand, within yhcH at 3367.260 kb on - strand, within yhcH at 3367.273 kb on - strand, within yhcH at 3367.273 kb on - strand, within yhcH at 3367.348 kb on - strand, within yhcH at 3367.386 kb on - strand, within yhcH at 3367.386 kb on - strand, within yhcH at 3367.444 kb on - strand, within yhcH at 3367.452 kb on - strand, within yhcH at 3367.454 kb on + strand at 3367.480 kb on - strand at 3367.480 kb on - strand at 3367.489 kb on + strand at 3367.523 kb on - strand at 3367.600 kb on + strand, within yhcI at 3367.600 kb on + strand, within yhcI at 3367.666 kb on - strand, within yhcI at 3367.678 kb on + strand, within yhcI at 3367.678 kb on + strand, within yhcI at 3367.691 kb on + strand, within yhcI at 3367.691 kb on + strand, within yhcI at 3367.725 kb on + strand, within yhcI at 3367.728 kb on + strand, within yhcI at 3367.768 kb on - strand, within yhcI at 3367.855 kb on - strand, within yhcI at 3367.855 kb on - strand, within yhcI at 3367.901 kb on + strand, within yhcI at 3368.002 kb on + strand, within yhcI at 3368.179 kb on - strand, within yhcI at 3368.179 kb on - strand, within yhcI at 3368.184 kb on - strand, within yhcI at 3368.285 kb on + strand at 3368.327 kb on - strand at 3368.368 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 3,366,036 - yhcG b3220 0.17 -0.3 3,366,036 - yhcG b3220 0.17 +0.5 3,366,046 + yhcG b3220 0.17 -2.1 3,366,055 + yhcG b3220 0.18 -0.3 3,366,055 + yhcG b3220 0.18 -1.3 3,366,079 + yhcG b3220 0.20 +0.9 3,366,091 + yhcG b3220 0.21 -0.3 3,366,091 + yhcG b3220 0.21 -0.1 3,366,184 + yhcG b3220 0.30 -0.1 3,366,275 + yhcG b3220 0.38 -0.5 3,366,275 + yhcG b3220 0.38 +3.0 3,366,355 - yhcG b3220 0.45 +0.2 3,366,355 - yhcG b3220 0.45 +0.5 3,366,359 - yhcG b3220 0.45 +0.5 3,366,399 + yhcG b3220 0.49 +0.7 3,366,404 + yhcG b3220 0.49 +1.5 3,366,404 + yhcG b3220 0.49 +1.2 3,366,409 - yhcG b3220 0.50 -0.2 3,366,446 + yhcG b3220 0.53 -0.9 3,366,484 - yhcG b3220 0.56 +0.9 3,366,484 - yhcG b3220 0.56 +0.1 3,366,683 - yhcG b3220 0.74 +0.5 3,366,683 - yhcG b3220 0.74 -0.5 3,366,684 + yhcG b3220 0.74 +0.5 3,366,684 + yhcG b3220 0.74 +0.9 3,366,692 - yhcG b3220 0.75 -0.5 3,366,720 + yhcG b3220 0.77 +0.5 3,366,760 + yhcG b3220 0.81 +2.0 3,366,760 + yhcG b3220 0.81 -0.6 3,366,808 + yhcG b3220 0.85 +0.5 3,366,856 - yhcG b3220 0.89 +0.2 3,366,857 + yhcG b3220 0.89 -0.8 3,366,906 + +1.5 3,366,924 - +1.0 3,366,955 - +1.0 3,366,960 + +0.1 3,366,960 + +0.5 3,366,960 + +0.5 3,366,967 - +0.2 3,366,968 - +0.5 3,367,219 - yhcH b3221 0.39 +0.5 3,367,221 + yhcH b3221 0.40 +0.7 3,367,238 + yhcH b3221 0.43 +2.3 3,367,246 - yhcH b3221 0.45 +0.6 3,367,260 - yhcH b3221 0.48 -0.3 3,367,273 - yhcH b3221 0.51 +0.0 3,367,273 - yhcH b3221 0.51 +0.0 3,367,348 - yhcH b3221 0.67 +1.0 3,367,386 - yhcH b3221 0.75 +0.5 3,367,386 - yhcH b3221 0.75 +0.5 3,367,444 - yhcH b3221 0.88 +1.0 3,367,452 - yhcH b3221 0.89 +0.6 3,367,454 + +0.2 3,367,480 - -0.1 3,367,480 - +0.9 3,367,489 + -0.2 3,367,523 - +0.1 3,367,600 + yhcI b3222 0.12 +0.2 3,367,600 + yhcI b3222 0.12 +1.8 3,367,666 - yhcI b3222 0.19 +0.2 3,367,678 + yhcI b3222 0.21 +0.8 3,367,678 + yhcI b3222 0.21 -0.7 3,367,691 + yhcI b3222 0.22 +0.9 3,367,691 + yhcI b3222 0.22 +1.5 3,367,725 + yhcI b3222 0.26 +0.8 3,367,728 + yhcI b3222 0.26 -1.4 3,367,768 - yhcI b3222 0.31 +0.5 3,367,855 - yhcI b3222 0.41 +1.5 3,367,855 - yhcI b3222 0.41 -1.3 3,367,901 + yhcI b3222 0.46 -0.0 3,368,002 + yhcI b3222 0.58 -0.5 3,368,179 - yhcI b3222 0.78 +0.3 3,368,179 - yhcI b3222 0.78 +1.0 3,368,184 - yhcI b3222 0.78 -1.1 3,368,285 + -0.2 3,368,327 - -1.3 3,368,368 + -0.9
Or see this region's nucleotide sequence