Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygbJ and ygbK overlap by 4 nucleotides ygbK and ygbL are separated by 91 nucleotides ygbL and ygbM are separated by 4 nucleotides
b2736: ygbJ - predicted dehydrogenase, with NAD(P)-binding Rossmann-fold domain (NCBI), at 2,859,452 to 2,860,360
ygbJ
b2737: ygbK - hypothetical protein (NCBI), at 2,860,357 to 2,861,523
ygbK
b2738: ygbL - hypothetical protein (NCBI), at 2,861,615 to 2,862,253
ygbL
b2739: ygbM - hypothetical protein (NCBI), at 2,862,258 to 2,863,034
ygbM
Position (kb)
2860
2861
2862 Strain fitness (log2 ratio)
-1
0
1
2 at 2859.528 kb on - strand at 2859.528 kb on - strand at 2859.539 kb on - strand at 2859.664 kb on + strand, within ygbJ at 2859.852 kb on - strand, within ygbJ at 2859.962 kb on - strand, within ygbJ at 2859.965 kb on + strand, within ygbJ at 2860.243 kb on + strand, within ygbJ at 2860.243 kb on + strand, within ygbJ at 2860.296 kb on + strand at 2860.464 kb on - strand at 2860.540 kb on - strand, within ygbK at 2860.598 kb on - strand, within ygbK at 2860.598 kb on - strand, within ygbK at 2860.657 kb on + strand, within ygbK at 2860.721 kb on + strand, within ygbK at 2860.847 kb on - strand, within ygbK at 2860.851 kb on + strand, within ygbK at 2860.895 kb on - strand, within ygbK at 2860.990 kb on + strand, within ygbK at 2860.990 kb on + strand, within ygbK at 2860.997 kb on + strand, within ygbK at 2860.998 kb on - strand, within ygbK at 2861.029 kb on - strand, within ygbK at 2861.044 kb on + strand, within ygbK at 2861.044 kb on + strand, within ygbK at 2861.080 kb on + strand, within ygbK at 2861.111 kb on + strand, within ygbK at 2861.166 kb on - strand, within ygbK at 2861.195 kb on + strand, within ygbK at 2861.301 kb on + strand, within ygbK at 2861.301 kb on + strand, within ygbK at 2861.518 kb on + strand at 2861.531 kb on + strand at 2861.531 kb on + strand at 2861.539 kb on - strand at 2861.539 kb on - strand at 2861.551 kb on + strand at 2861.602 kb on - strand at 2861.614 kb on - strand at 2861.614 kb on - strand at 2861.719 kb on - strand, within ygbL at 2861.766 kb on - strand, within ygbL at 2861.778 kb on + strand, within ygbL at 2861.778 kb on + strand, within ygbL at 2861.832 kb on + strand, within ygbL at 2861.924 kb on - strand, within ygbL at 2861.984 kb on - strand, within ygbL at 2862.001 kb on + strand, within ygbL at 2862.001 kb on + strand, within ygbL at 2862.384 kb on - strand, within ygbM at 2862.439 kb on - strand, within ygbM at 2862.439 kb on - strand, within ygbM at 2862.479 kb on - strand, within ygbM at 2862.511 kb on - strand, within ygbM
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 2,859,528 - -0.2 2,859,528 - +1.0 2,859,539 - +0.7 2,859,664 + ygbJ b2736 0.23 +0.3 2,859,852 - ygbJ b2736 0.44 -0.2 2,859,962 - ygbJ b2736 0.56 +0.2 2,859,965 + ygbJ b2736 0.56 +0.2 2,860,243 + ygbJ b2736 0.87 +0.7 2,860,243 + ygbJ b2736 0.87 -0.3 2,860,296 + +0.6 2,860,464 - +0.9 2,860,540 - ygbK b2737 0.16 +0.3 2,860,598 - ygbK b2737 0.21 +0.7 2,860,598 - ygbK b2737 0.21 +0.3 2,860,657 + ygbK b2737 0.26 +1.3 2,860,721 + ygbK b2737 0.31 +1.3 2,860,847 - ygbK b2737 0.42 -0.2 2,860,851 + ygbK b2737 0.42 +0.1 2,860,895 - ygbK b2737 0.46 -1.3 2,860,990 + ygbK b2737 0.54 -1.3 2,860,990 + ygbK b2737 0.54 +0.3 2,860,997 + ygbK b2737 0.55 +0.3 2,860,998 - ygbK b2737 0.55 +0.9 2,861,029 - ygbK b2737 0.58 +1.7 2,861,044 + ygbK b2737 0.59 +1.6 2,861,044 + ygbK b2737 0.59 +0.3 2,861,080 + ygbK b2737 0.62 -1.5 2,861,111 + ygbK b2737 0.65 +0.3 2,861,166 - ygbK b2737 0.69 +0.0 2,861,195 + ygbK b2737 0.72 -0.4 2,861,301 + ygbK b2737 0.81 +2.3 2,861,301 + ygbK b2737 0.81 -0.0 2,861,518 + +0.1 2,861,531 + -0.1 2,861,531 + -0.1 2,861,539 - +0.3 2,861,539 - +0.9 2,861,551 + -0.5 2,861,602 - +1.3 2,861,614 - -0.7 2,861,614 - -1.5 2,861,719 - ygbL b2738 0.16 +0.8 2,861,766 - ygbL b2738 0.24 -0.2 2,861,778 + ygbL b2738 0.26 +0.3 2,861,778 + ygbL b2738 0.26 +1.5 2,861,832 + ygbL b2738 0.34 -1.1 2,861,924 - ygbL b2738 0.48 -1.5 2,861,984 - ygbL b2738 0.58 +1.3 2,862,001 + ygbL b2738 0.60 +0.3 2,862,001 + ygbL b2738 0.60 +0.7 2,862,384 - ygbM b2739 0.16 +1.0 2,862,439 - ygbM b2739 0.23 +0.3 2,862,439 - ygbM b2739 0.23 -0.4 2,862,479 - ygbM b2739 0.28 +0.1 2,862,511 - ygbM b2739 0.33 +1.3
Or see this region's nucleotide sequence