Strain Fitness in Escherichia coli BW25113 around b2737

Experiment: Doxycycline hyclate 0.001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygbJ and ygbK overlap by 4 nucleotidesygbK and ygbL are separated by 91 nucleotidesygbL and ygbM are separated by 4 nucleotides b2736: ygbJ - predicted dehydrogenase, with NAD(P)-binding Rossmann-fold domain (NCBI), at 2,859,452 to 2,860,360 ygbJ b2737: ygbK - hypothetical protein (NCBI), at 2,860,357 to 2,861,523 ygbK b2738: ygbL - hypothetical protein (NCBI), at 2,861,615 to 2,862,253 ygbL b2739: ygbM - hypothetical protein (NCBI), at 2,862,258 to 2,863,034 ygbM Position (kb) 2860 2861 2862Strain fitness (log2 ratio) -1 0 1 2at 2859.528 kb on - strandat 2859.528 kb on - strandat 2859.539 kb on - strandat 2859.664 kb on + strand, within ygbJat 2859.852 kb on - strand, within ygbJat 2859.962 kb on - strand, within ygbJat 2859.965 kb on + strand, within ygbJat 2860.243 kb on + strand, within ygbJat 2860.243 kb on + strand, within ygbJat 2860.296 kb on + strandat 2860.464 kb on - strandat 2860.540 kb on - strand, within ygbKat 2860.598 kb on - strand, within ygbKat 2860.598 kb on - strand, within ygbKat 2860.657 kb on + strand, within ygbKat 2860.721 kb on + strand, within ygbKat 2860.847 kb on - strand, within ygbKat 2860.851 kb on + strand, within ygbKat 2860.895 kb on - strand, within ygbKat 2860.990 kb on + strand, within ygbKat 2860.990 kb on + strand, within ygbKat 2860.997 kb on + strand, within ygbKat 2860.998 kb on - strand, within ygbKat 2861.029 kb on - strand, within ygbKat 2861.044 kb on + strand, within ygbKat 2861.044 kb on + strand, within ygbKat 2861.080 kb on + strand, within ygbKat 2861.111 kb on + strand, within ygbKat 2861.166 kb on - strand, within ygbKat 2861.195 kb on + strand, within ygbKat 2861.301 kb on + strand, within ygbKat 2861.301 kb on + strand, within ygbKat 2861.518 kb on + strandat 2861.531 kb on + strandat 2861.531 kb on + strandat 2861.539 kb on - strandat 2861.539 kb on - strandat 2861.551 kb on + strandat 2861.602 kb on - strandat 2861.614 kb on - strandat 2861.614 kb on - strandat 2861.719 kb on - strand, within ygbLat 2861.766 kb on - strand, within ygbLat 2861.778 kb on + strand, within ygbLat 2861.778 kb on + strand, within ygbLat 2861.832 kb on + strand, within ygbLat 2861.924 kb on - strand, within ygbLat 2861.984 kb on - strand, within ygbLat 2862.001 kb on + strand, within ygbLat 2862.001 kb on + strand, within ygbLat 2862.384 kb on - strand, within ygbMat 2862.439 kb on - strand, within ygbMat 2862.439 kb on - strand, within ygbMat 2862.479 kb on - strand, within ygbMat 2862.511 kb on - strand, within ygbM

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml
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2,859,528 - -0.2
2,859,528 - +1.0
2,859,539 - +0.7
2,859,664 + ygbJ b2736 0.23 +0.3
2,859,852 - ygbJ b2736 0.44 -0.2
2,859,962 - ygbJ b2736 0.56 +0.2
2,859,965 + ygbJ b2736 0.56 +0.2
2,860,243 + ygbJ b2736 0.87 +0.7
2,860,243 + ygbJ b2736 0.87 -0.3
2,860,296 + +0.6
2,860,464 - +0.9
2,860,540 - ygbK b2737 0.16 +0.3
2,860,598 - ygbK b2737 0.21 +0.7
2,860,598 - ygbK b2737 0.21 +0.3
2,860,657 + ygbK b2737 0.26 +1.3
2,860,721 + ygbK b2737 0.31 +1.3
2,860,847 - ygbK b2737 0.42 -0.2
2,860,851 + ygbK b2737 0.42 +0.1
2,860,895 - ygbK b2737 0.46 -1.3
2,860,990 + ygbK b2737 0.54 -1.3
2,860,990 + ygbK b2737 0.54 +0.3
2,860,997 + ygbK b2737 0.55 +0.3
2,860,998 - ygbK b2737 0.55 +0.9
2,861,029 - ygbK b2737 0.58 +1.7
2,861,044 + ygbK b2737 0.59 +1.6
2,861,044 + ygbK b2737 0.59 +0.3
2,861,080 + ygbK b2737 0.62 -1.5
2,861,111 + ygbK b2737 0.65 +0.3
2,861,166 - ygbK b2737 0.69 +0.0
2,861,195 + ygbK b2737 0.72 -0.4
2,861,301 + ygbK b2737 0.81 +2.3
2,861,301 + ygbK b2737 0.81 -0.0
2,861,518 + +0.1
2,861,531 + -0.1
2,861,531 + -0.1
2,861,539 - +0.3
2,861,539 - +0.9
2,861,551 + -0.5
2,861,602 - +1.3
2,861,614 - -0.7
2,861,614 - -1.5
2,861,719 - ygbL b2738 0.16 +0.8
2,861,766 - ygbL b2738 0.24 -0.2
2,861,778 + ygbL b2738 0.26 +0.3
2,861,778 + ygbL b2738 0.26 +1.5
2,861,832 + ygbL b2738 0.34 -1.1
2,861,924 - ygbL b2738 0.48 -1.5
2,861,984 - ygbL b2738 0.58 +1.3
2,862,001 + ygbL b2738 0.60 +0.3
2,862,001 + ygbL b2738 0.60 +0.7
2,862,384 - ygbM b2739 0.16 +1.0
2,862,439 - ygbM b2739 0.23 +0.3
2,862,439 - ygbM b2739 0.23 -0.4
2,862,479 - ygbM b2739 0.28 +0.1
2,862,511 - ygbM b2739 0.33 +1.3

Or see this region's nucleotide sequence