Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt clpB and ryfD overlap by 21 nucleotides ryfD and yfiH are separated by 7 nucleotides yfiH and rluD overlap by 4 nucleotides
b2592: clpB - protein disaggregation chaperone (NCBI), at 2,729,622 to 2,732,195
clpB
b4609: ryfD - Novel sRNA, function unknown (RefSeq), at 2,732,175 to 2,732,317
ryfD
b2593: yfiH - hypothetical protein (NCBI), at 2,732,325 to 2,733,056
yfiH
b2594: rluD - 23S rRNA pseudouridine synthase (NCBI), at 2,733,053 to 2,734,033
rluD
Position (kb)
2732
2733
2734 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2731.351 kb on + strand, within clpB at 2731.448 kb on - strand, within clpB at 2731.468 kb on + strand, within clpB at 2731.476 kb on - strand, within clpB at 2731.476 kb on - strand, within clpB at 2731.481 kb on - strand, within clpB at 2731.531 kb on - strand, within clpB at 2731.575 kb on - strand, within clpB at 2731.674 kb on - strand, within clpB at 2731.730 kb on + strand, within clpB at 2731.736 kb on - strand, within clpB at 2731.746 kb on - strand, within clpB at 2731.746 kb on - strand, within clpB at 2731.857 kb on - strand, within clpB at 2731.868 kb on + strand, within clpB at 2731.919 kb on + strand, within clpB at 2731.927 kb on - strand, within clpB at 2731.965 kb on - strand at 2731.966 kb on - strand at 2731.966 kb on - strand at 2732.002 kb on - strand at 2732.140 kb on - strand at 2732.202 kb on + strand, within ryfD at 2732.205 kb on - strand, within ryfD at 2732.212 kb on + strand, within ryfD at 2732.212 kb on + strand, within ryfD at 2732.251 kb on + strand, within ryfD at 2732.277 kb on - strand, within ryfD at 2732.277 kb on - strand, within ryfD at 2732.323 kb on + strand at 2732.323 kb on + strand at 2732.456 kb on - strand, within yfiH at 2732.499 kb on - strand, within yfiH at 2732.499 kb on - strand, within yfiH at 2732.502 kb on - strand, within yfiH at 2732.502 kb on - strand, within yfiH at 2732.563 kb on - strand, within yfiH at 2732.563 kb on - strand, within yfiH at 2732.594 kb on + strand, within yfiH at 2732.602 kb on - strand, within yfiH at 2732.648 kb on + strand, within yfiH at 2732.659 kb on + strand, within yfiH at 2732.669 kb on + strand, within yfiH at 2732.748 kb on + strand, within yfiH at 2732.756 kb on - strand, within yfiH at 2732.763 kb on - strand, within yfiH at 2732.763 kb on - strand, within yfiH at 2732.778 kb on + strand, within yfiH at 2732.813 kb on - strand, within yfiH at 2732.813 kb on - strand, within yfiH at 2732.852 kb on - strand, within yfiH at 2732.854 kb on - strand, within yfiH at 2732.926 kb on + strand, within yfiH at 2732.943 kb on - strand, within yfiH at 2732.969 kb on - strand, within yfiH at 2732.969 kb on - strand, within yfiH at 2732.994 kb on + strand at 2733.052 kb on + strand at 2733.060 kb on - strand at 2733.062 kb on - strand at 2733.072 kb on + strand at 2733.079 kb on - strand at 2733.079 kb on - strand at 2734.040 kb on - strand at 2734.040 kb on - strand at 2734.042 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 2,731,351 + clpB b2592 0.67 -0.1 2,731,448 - clpB b2592 0.71 -1.7 2,731,468 + clpB b2592 0.72 -0.6 2,731,476 - clpB b2592 0.72 -0.7 2,731,476 - clpB b2592 0.72 -0.6 2,731,481 - clpB b2592 0.72 +1.9 2,731,531 - clpB b2592 0.74 -0.1 2,731,575 - clpB b2592 0.76 +0.3 2,731,674 - clpB b2592 0.80 -0.1 2,731,730 + clpB b2592 0.82 -1.0 2,731,736 - clpB b2592 0.82 +0.3 2,731,746 - clpB b2592 0.83 +0.5 2,731,746 - clpB b2592 0.83 -0.7 2,731,857 - clpB b2592 0.87 -0.2 2,731,868 + clpB b2592 0.87 +0.0 2,731,919 + clpB b2592 0.89 +0.0 2,731,927 - clpB b2592 0.90 -0.3 2,731,965 - +0.3 2,731,966 - -0.7 2,731,966 - +0.9 2,732,002 - -0.7 2,732,140 - +0.3 2,732,202 + ryfD b4609 0.19 -2.3 2,732,205 - ryfD b4609 0.21 +0.7 2,732,212 + ryfD b4609 0.26 -0.7 2,732,212 + ryfD b4609 0.26 -1.3 2,732,251 + ryfD b4609 0.53 -0.6 2,732,277 - ryfD b4609 0.71 +0.3 2,732,277 - ryfD b4609 0.71 +1.3 2,732,323 + -1.1 2,732,323 + -0.3 2,732,456 - yfiH b2593 0.18 +2.4 2,732,499 - yfiH b2593 0.24 +1.9 2,732,499 - yfiH b2593 0.24 +0.3 2,732,502 - yfiH b2593 0.24 -0.7 2,732,502 - yfiH b2593 0.24 +2.9 2,732,563 - yfiH b2593 0.33 +0.9 2,732,563 - yfiH b2593 0.33 +0.9 2,732,594 + yfiH b2593 0.37 +2.4 2,732,602 - yfiH b2593 0.38 +1.2 2,732,648 + yfiH b2593 0.44 +1.4 2,732,659 + yfiH b2593 0.46 +1.3 2,732,669 + yfiH b2593 0.47 +1.0 2,732,748 + yfiH b2593 0.58 +1.0 2,732,756 - yfiH b2593 0.59 +1.0 2,732,763 - yfiH b2593 0.60 -1.1 2,732,763 - yfiH b2593 0.60 +1.5 2,732,778 + yfiH b2593 0.62 +1.3 2,732,813 - yfiH b2593 0.67 +0.5 2,732,813 - yfiH b2593 0.67 -0.3 2,732,852 - yfiH b2593 0.72 +0.0 2,732,854 - yfiH b2593 0.72 +1.0 2,732,926 + yfiH b2593 0.82 +0.2 2,732,943 - yfiH b2593 0.84 +1.5 2,732,969 - yfiH b2593 0.88 +0.9 2,732,969 - yfiH b2593 0.88 +0.3 2,732,994 + +1.0 2,733,052 + -0.7 2,733,060 - -1.1 2,733,062 - +0.3 2,733,072 + +0.6 2,733,079 - +0.1 2,733,079 - -1.6 2,734,040 - -0.5 2,734,040 - -0.9 2,734,042 - +0.3
Or see this region's nucleotide sequence