Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt eutA and eutH overlap by 4 nucleotides eutH and eutG are separated by 216 nucleotides
b2451: eutA - reactivating factor for ethanolamine ammonia lyase (NCBI), at 2,563,503 to 2,564,906
eutA
b2452: eutH - predicted inner membrane protein (NCBI), at 2,564,903 to 2,566,129
eutH
b2453: eutG - ethanolamine utilization; homolog of Salmonella enzyme, similar to iron-containing alcohol dehydrogenase (VIMSS), at 2,566,346 to 2,567,533
eutG
Position (kb)
2564
2565
2566
2567 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2563.913 kb on - strand, within eutA at 2563.949 kb on - strand, within eutA at 2564.001 kb on + strand, within eutA at 2564.037 kb on - strand, within eutA at 2564.285 kb on + strand, within eutA at 2564.377 kb on - strand, within eutA at 2564.443 kb on - strand, within eutA at 2564.443 kb on - strand, within eutA at 2564.498 kb on - strand, within eutA at 2564.498 kb on - strand, within eutA at 2564.577 kb on - strand, within eutA at 2564.599 kb on + strand, within eutA at 2564.626 kb on + strand, within eutA at 2564.636 kb on - strand, within eutA at 2564.636 kb on - strand, within eutA at 2564.741 kb on + strand, within eutA at 2564.743 kb on + strand, within eutA at 2564.753 kb on - strand, within eutA at 2564.865 kb on + strand at 2565.050 kb on + strand, within eutH at 2565.126 kb on + strand, within eutH at 2565.171 kb on - strand, within eutH at 2565.210 kb on - strand, within eutH at 2565.259 kb on - strand, within eutH at 2565.275 kb on + strand, within eutH at 2565.275 kb on + strand, within eutH at 2565.331 kb on + strand, within eutH at 2565.353 kb on - strand, within eutH at 2565.416 kb on + strand, within eutH at 2565.454 kb on - strand, within eutH at 2565.454 kb on - strand, within eutH at 2565.593 kb on + strand, within eutH at 2565.593 kb on + strand, within eutH at 2565.770 kb on - strand, within eutH at 2565.772 kb on - strand, within eutH at 2565.783 kb on - strand, within eutH at 2565.838 kb on - strand, within eutH at 2565.840 kb on + strand, within eutH at 2565.931 kb on + strand, within eutH at 2565.948 kb on + strand, within eutH at 2565.981 kb on + strand, within eutH at 2565.981 kb on + strand, within eutH at 2566.315 kb on + strand at 2566.315 kb on + strand at 2566.364 kb on + strand at 2566.409 kb on - strand at 2566.431 kb on + strand at 2566.451 kb on - strand at 2566.515 kb on + strand, within eutG at 2566.522 kb on + strand, within eutG at 2566.522 kb on + strand, within eutG at 2566.585 kb on + strand, within eutG at 2566.620 kb on + strand, within eutG at 2566.620 kb on + strand, within eutG at 2566.637 kb on + strand, within eutG at 2566.894 kb on + strand, within eutG at 2566.914 kb on + strand, within eutG at 2566.938 kb on - strand, within eutG at 2566.938 kb on - strand, within eutG at 2566.938 kb on - strand, within eutG at 2567.008 kb on + strand, within eutG at 2567.012 kb on - strand, within eutG
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 2,563,913 - eutA b2451 0.29 -0.8 2,563,949 - eutA b2451 0.32 -1.0 2,564,001 + eutA b2451 0.35 +0.2 2,564,037 - eutA b2451 0.38 +0.2 2,564,285 + eutA b2451 0.56 +0.1 2,564,377 - eutA b2451 0.62 -0.0 2,564,443 - eutA b2451 0.67 -0.8 2,564,443 - eutA b2451 0.67 +1.8 2,564,498 - eutA b2451 0.71 +1.0 2,564,498 - eutA b2451 0.71 -0.8 2,564,577 - eutA b2451 0.76 -0.2 2,564,599 + eutA b2451 0.78 -0.2 2,564,626 + eutA b2451 0.80 -0.1 2,564,636 - eutA b2451 0.81 -1.2 2,564,636 - eutA b2451 0.81 -0.2 2,564,741 + eutA b2451 0.88 -0.3 2,564,743 + eutA b2451 0.88 +0.4 2,564,753 - eutA b2451 0.89 -1.3 2,564,865 + +0.5 2,565,050 + eutH b2452 0.12 +0.5 2,565,126 + eutH b2452 0.18 +0.7 2,565,171 - eutH b2452 0.22 +0.5 2,565,210 - eutH b2452 0.25 +0.0 2,565,259 - eutH b2452 0.29 +1.2 2,565,275 + eutH b2452 0.30 +2.2 2,565,275 + eutH b2452 0.30 +0.7 2,565,331 + eutH b2452 0.35 +0.9 2,565,353 - eutH b2452 0.37 +1.1 2,565,416 + eutH b2452 0.42 -0.3 2,565,454 - eutH b2452 0.45 +0.2 2,565,454 - eutH b2452 0.45 -0.0 2,565,593 + eutH b2452 0.56 +2.8 2,565,593 + eutH b2452 0.56 +0.0 2,565,770 - eutH b2452 0.71 +1.0 2,565,772 - eutH b2452 0.71 +0.7 2,565,783 - eutH b2452 0.72 +0.5 2,565,838 - eutH b2452 0.76 +0.2 2,565,840 + eutH b2452 0.76 -0.1 2,565,931 + eutH b2452 0.84 +0.1 2,565,948 + eutH b2452 0.85 -0.8 2,565,981 + eutH b2452 0.88 +0.2 2,565,981 + eutH b2452 0.88 +1.8 2,566,315 + -0.3 2,566,315 + -0.1 2,566,364 + -0.3 2,566,409 - +0.1 2,566,431 + -0.5 2,566,451 - -1.3 2,566,515 + eutG b2453 0.14 -0.9 2,566,522 + eutG b2453 0.15 +0.7 2,566,522 + eutG b2453 0.15 +0.2 2,566,585 + eutG b2453 0.20 +0.2 2,566,620 + eutG b2453 0.23 -0.8 2,566,620 + eutG b2453 0.23 -2.3 2,566,637 + eutG b2453 0.24 +0.4 2,566,894 + eutG b2453 0.46 +1.0 2,566,914 + eutG b2453 0.48 -0.0 2,566,938 - eutG b2453 0.50 +0.2 2,566,938 - eutG b2453 0.50 -0.6 2,566,938 - eutG b2453 0.50 +1.6 2,567,008 + eutG b2453 0.56 -0.8 2,567,012 - eutG b2453 0.56 -1.8
Or see this region's nucleotide sequence