Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdxB and flk are separated by 98 nucleotides flk and yfcJ overlap by 4 nucleotides
b2320: pdxB - erythronate-4-phosphate dehydrogenase (NCBI), at 2,434,737 to 2,435,873
pdxB
b2321: flk - predicted flagella assembly protein (NCBI), at 2,435,972 to 2,436,967
flk
b2322: yfcJ - predicted transporter (NCBI), at 2,436,964 to 2,438,142
yfcJ
Position (kb)
2435
2436
2437 Strain fitness (log2 ratio)
-2
-1
0
1 at 2434.999 kb on - strand, within pdxB at 2435.038 kb on - strand, within pdxB at 2435.105 kb on - strand at 2435.110 kb on + strand, within pdxB at 2435.110 kb on + strand, within pdxB at 2435.230 kb on + strand, within pdxB at 2435.230 kb on + strand, within pdxB at 2435.246 kb on + strand, within pdxB at 2435.285 kb on + strand, within pdxB at 2435.324 kb on + strand, within pdxB at 2435.324 kb on + strand, within pdxB at 2435.343 kb on - strand, within pdxB at 2435.511 kb on + strand, within pdxB at 2435.796 kb on - strand at 2435.796 kb on - strand at 2435.801 kb on - strand at 2435.838 kb on + strand at 2435.838 kb on + strand at 2435.913 kb on - strand at 2435.939 kb on - strand at 2435.982 kb on - strand at 2436.015 kb on - strand at 2436.067 kb on + strand at 2436.124 kb on - strand, within flk at 2436.134 kb on - strand, within flk at 2436.134 kb on - strand, within flk at 2436.134 kb on - strand, within flk at 2436.164 kb on - strand, within flk at 2436.211 kb on + strand, within flk at 2436.258 kb on - strand, within flk at 2436.258 kb on - strand, within flk at 2436.260 kb on + strand, within flk at 2436.352 kb on - strand, within flk at 2436.352 kb on - strand, within flk at 2436.359 kb on + strand, within flk at 2436.359 kb on + strand, within flk at 2436.629 kb on - strand, within flk at 2436.774 kb on + strand, within flk at 2436.813 kb on - strand, within flk at 2436.813 kb on - strand, within flk at 2436.828 kb on + strand, within flk at 2436.914 kb on - strand at 2436.923 kb on + strand at 2436.923 kb on + strand at 2436.927 kb on - strand at 2436.933 kb on + strand at 2436.941 kb on - strand at 2436.947 kb on - strand at 2436.954 kb on + strand at 2436.993 kb on + strand at 2437.001 kb on - strand at 2437.001 kb on - strand at 2437.008 kb on + strand at 2437.207 kb on + strand, within yfcJ at 2437.207 kb on + strand, within yfcJ at 2437.285 kb on + strand, within yfcJ at 2437.287 kb on - strand, within yfcJ at 2437.329 kb on - strand, within yfcJ at 2437.389 kb on - strand, within yfcJ at 2437.446 kb on - strand, within yfcJ at 2437.471 kb on + strand, within yfcJ at 2437.534 kb on + strand, within yfcJ at 2437.574 kb on + strand, within yfcJ at 2437.687 kb on - strand, within yfcJ at 2437.818 kb on + strand, within yfcJ at 2437.818 kb on + strand, within yfcJ at 2437.818 kb on + strand, within yfcJ at 2437.917 kb on - strand, within yfcJ
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 2,434,999 - pdxB b2320 0.23 +0.2 2,435,038 - pdxB b2320 0.26 +0.6 2,435,105 - +0.2 2,435,110 + pdxB b2320 0.33 -1.7 2,435,110 + pdxB b2320 0.33 -1.4 2,435,230 + pdxB b2320 0.43 -0.2 2,435,230 + pdxB b2320 0.43 +1.5 2,435,246 + pdxB b2320 0.45 -0.8 2,435,285 + pdxB b2320 0.48 -0.1 2,435,324 + pdxB b2320 0.52 +0.4 2,435,324 + pdxB b2320 0.52 +0.2 2,435,343 - pdxB b2320 0.53 +0.7 2,435,511 + pdxB b2320 0.68 +0.5 2,435,796 - +0.2 2,435,796 - +1.5 2,435,801 - +0.2 2,435,838 + +1.0 2,435,838 + -1.5 2,435,913 - +0.9 2,435,939 - +0.4 2,435,982 - +0.2 2,436,015 - -0.5 2,436,067 + +1.1 2,436,124 - flk b2321 0.15 +1.4 2,436,134 - flk b2321 0.16 -1.4 2,436,134 - flk b2321 0.16 +1.0 2,436,134 - flk b2321 0.16 +1.2 2,436,164 - flk b2321 0.19 +1.2 2,436,211 + flk b2321 0.24 +0.6 2,436,258 - flk b2321 0.29 -1.4 2,436,258 - flk b2321 0.29 +1.2 2,436,260 + flk b2321 0.29 -0.1 2,436,352 - flk b2321 0.38 +0.4 2,436,352 - flk b2321 0.38 -0.8 2,436,359 + flk b2321 0.39 +0.2 2,436,359 + flk b2321 0.39 +0.2 2,436,629 - flk b2321 0.66 +0.5 2,436,774 + flk b2321 0.81 -0.2 2,436,813 - flk b2321 0.84 +0.2 2,436,813 - flk b2321 0.84 +0.6 2,436,828 + flk b2321 0.86 -0.8 2,436,914 - -0.8 2,436,923 + -2.1 2,436,923 + +0.8 2,436,927 - +0.1 2,436,933 + -0.8 2,436,941 - -2.1 2,436,947 - +0.6 2,436,954 + +0.4 2,436,993 + +0.4 2,437,001 - -0.2 2,437,001 - -0.4 2,437,008 + +0.2 2,437,207 + yfcJ b2322 0.21 -0.8 2,437,207 + yfcJ b2322 0.21 +0.2 2,437,285 + yfcJ b2322 0.27 -1.8 2,437,287 - yfcJ b2322 0.27 +0.6 2,437,329 - yfcJ b2322 0.31 -0.4 2,437,389 - yfcJ b2322 0.36 -0.3 2,437,446 - yfcJ b2322 0.41 -1.0 2,437,471 + yfcJ b2322 0.43 -0.5 2,437,534 + yfcJ b2322 0.48 +0.0 2,437,574 + yfcJ b2322 0.52 -1.4 2,437,687 - yfcJ b2322 0.61 +0.8 2,437,818 + yfcJ b2322 0.72 -0.4 2,437,818 + yfcJ b2322 0.72 +0.2 2,437,818 + yfcJ b2322 0.72 +0.4 2,437,917 - yfcJ b2322 0.81 -0.3
Or see this region's nucleotide sequence