Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt xthA and ydjX are separated by 166 nucleotides ydjX and ydjY are separated by 4 nucleotides ydjY and ydjZ are separated by 14 nucleotides ydjZ and ynjA overlap by 1 nucleotides
b1749: xthA - exonuclease III (NCBI), at 1,830,452 to 1,831,258
xthA
b1750: ydjX - orf, hypothetical protein (VIMSS), at 1,831,425 to 1,832,135
ydjX
b1751: ydjY - orf, hypothetical protein (VIMSS), at 1,832,140 to 1,832,817
ydjY
b1752: ydjZ - conserved inner membrane protein (NCBI), at 1,832,832 to 1,833,539
ydjZ
b1753: ynjA - hypothetical protein (NCBI), at 1,833,539 to 1,834,087
ynjA
Position (kb)
1832
1833 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1831.243 kb on - strand at 1831.243 kb on - strand at 1831.277 kb on - strand at 1831.277 kb on - strand at 1831.351 kb on + strand at 1831.351 kb on + strand at 1831.504 kb on - strand, within ydjX at 1831.535 kb on - strand, within ydjX at 1831.535 kb on - strand, within ydjX at 1831.539 kb on - strand, within ydjX at 1831.642 kb on + strand, within ydjX at 1831.658 kb on + strand, within ydjX at 1831.679 kb on - strand, within ydjX at 1831.737 kb on - strand, within ydjX at 1831.737 kb on - strand, within ydjX at 1831.766 kb on + strand, within ydjX at 1831.766 kb on + strand, within ydjX at 1831.775 kb on + strand, within ydjX at 1831.782 kb on + strand, within ydjX at 1831.827 kb on - strand, within ydjX at 1831.834 kb on + strand, within ydjX at 1831.992 kb on - strand, within ydjX at 1832.016 kb on + strand, within ydjX at 1832.016 kb on + strand, within ydjX at 1832.084 kb on - strand at 1832.113 kb on - strand at 1832.113 kb on - strand at 1832.187 kb on + strand at 1832.403 kb on + strand, within ydjY at 1832.456 kb on + strand, within ydjY at 1832.456 kb on + strand, within ydjY at 1832.456 kb on + strand, within ydjY at 1832.456 kb on + strand, within ydjY at 1832.475 kb on - strand, within ydjY at 1832.475 kb on - strand, within ydjY at 1832.682 kb on + strand, within ydjY at 1832.707 kb on - strand, within ydjY at 1833.245 kb on + strand, within ydjZ at 1833.394 kb on - strand, within ydjZ at 1833.430 kb on - strand, within ydjZ at 1833.789 kb on + strand, within ynjA at 1833.789 kb on + strand, within ynjA
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 1,831,243 - +0.1 1,831,243 - +1.1 1,831,277 - +1.1 1,831,277 - +0.8 1,831,351 + +0.1 1,831,351 + +0.5 1,831,504 - ydjX b1750 0.11 -0.9 1,831,535 - ydjX b1750 0.15 +0.1 1,831,535 - ydjX b1750 0.15 +0.1 1,831,539 - ydjX b1750 0.16 +0.1 1,831,642 + ydjX b1750 0.31 +0.6 1,831,658 + ydjX b1750 0.33 +1.3 1,831,679 - ydjX b1750 0.36 +0.9 1,831,737 - ydjX b1750 0.44 +0.4 1,831,737 - ydjX b1750 0.44 +0.6 1,831,766 + ydjX b1750 0.48 -0.6 1,831,766 + ydjX b1750 0.48 -0.4 1,831,775 + ydjX b1750 0.49 +0.1 1,831,782 + ydjX b1750 0.50 -1.9 1,831,827 - ydjX b1750 0.57 -0.9 1,831,834 + ydjX b1750 0.58 +0.1 1,831,992 - ydjX b1750 0.80 -0.2 1,832,016 + ydjX b1750 0.83 +0.1 1,832,016 + ydjX b1750 0.83 -0.5 1,832,084 - -0.3 1,832,113 - +1.7 1,832,113 - +0.1 1,832,187 + +0.1 1,832,403 + ydjY b1751 0.39 +1.7 1,832,456 + ydjY b1751 0.47 +0.7 1,832,456 + ydjY b1751 0.47 -0.9 1,832,456 + ydjY b1751 0.47 +0.1 1,832,456 + ydjY b1751 0.47 +0.1 1,832,475 - ydjY b1751 0.49 +1.7 1,832,475 - ydjY b1751 0.49 -1.6 1,832,682 + ydjY b1751 0.80 +2.7 1,832,707 - ydjY b1751 0.84 +1.2 1,833,245 + ydjZ b1752 0.58 -1.8 1,833,394 - ydjZ b1752 0.79 -0.2 1,833,430 - ydjZ b1752 0.84 +0.1 1,833,789 + ynjA b1753 0.46 +0.1 1,833,789 + ynjA b1753 0.46 +0.8
Or see this region's nucleotide sequence