Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydiR and ydiS are separated by 55 nucleotides ydiS and ydiT overlap by 4 nucleotides ydiT and fadK are separated by 56 nucleotides
b1698: ydiR - predicted electron transfer flavoprotein, FAD-binding (NCBI), at 1,778,425 to 1,779,363
ydiR
b1699: ydiS - predicted oxidoreductase with FAD/NAD(P)-binding domain (NCBI), at 1,779,419 to 1,780,708
ydiS
b1700: ydiT - predicted 4Fe-4S ferredoxin-type protein (NCBI), at 1,780,705 to 1,780,998
ydiT
b1701: fadK - short chain acyl-CoA synthetase, anaerobic (RefSeq), at 1,781,055 to 1,782,701
fadK
Position (kb)
1779
1780
1781 Strain fitness (log2 ratio)
-1
0
1
2 at 1778.469 kb on + strand at 1778.740 kb on + strand, within ydiR at 1778.933 kb on - strand, within ydiR at 1779.145 kb on + strand, within ydiR at 1779.222 kb on - strand, within ydiR at 1779.423 kb on - strand at 1779.424 kb on - strand at 1779.424 kb on - strand at 1779.484 kb on + strand at 1779.721 kb on + strand, within ydiS at 1779.847 kb on + strand, within ydiS at 1780.127 kb on - strand, within ydiS at 1780.378 kb on - strand, within ydiS at 1780.483 kb on - strand, within ydiS at 1780.483 kb on - strand, within ydiS at 1780.566 kb on - strand, within ydiS at 1780.580 kb on + strand at 1780.598 kb on + strand at 1780.939 kb on + strand, within ydiT at 1781.119 kb on - strand at 1781.209 kb on - strand at 1781.217 kb on - strand at 1781.266 kb on + strand, within fadK at 1781.399 kb on - strand, within fadK at 1781.530 kb on + strand, within fadK
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 1,778,469 + -0.1 1,778,740 + ydiR b1698 0.34 -0.4 1,778,933 - ydiR b1698 0.54 +0.1 1,779,145 + ydiR b1698 0.77 -1.5 1,779,222 - ydiR b1698 0.85 +0.4 1,779,423 - +0.9 1,779,424 - -1.5 1,779,424 - -1.2 1,779,484 + -0.1 1,779,721 + ydiS b1699 0.23 -0.7 1,779,847 + ydiS b1699 0.33 +0.7 1,780,127 - ydiS b1699 0.55 +0.4 1,780,378 - ydiS b1699 0.74 +0.9 1,780,483 - ydiS b1699 0.82 +0.8 1,780,483 - ydiS b1699 0.82 +0.9 1,780,566 - ydiS b1699 0.89 +2.4 1,780,580 + +1.1 1,780,598 + +0.1 1,780,939 + ydiT b1700 0.80 +0.1 1,781,119 - -0.9 1,781,209 - -0.8 1,781,217 - +1.1 1,781,266 + fadK b1701 0.13 +0.7 1,781,399 - fadK b1701 0.21 +1.6 1,781,530 + fadK b1701 0.29 -0.9
Or see this region's nucleotide sequence