Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydgC and rstA are separated by 128 nucleotides rstA and rstB are separated by 3 nucleotides rstB and tus are separated by 75 nucleotides
b1607: ydgC - conserved inner membrane protein associated with alginate biosynthesis (NCBI), at 1,679,719 to 1,680,054
ydgC
b1608: rstA - DNA-binding response regulator in two-component regulatory system with RstB (RefSeq), at 1,680,183 to 1,680,902
rstA
b1609: rstB - sensory histidine kinase in two-component regulatory system with RstA (NCBI), at 1,680,906 to 1,682,207
rstB
b1610: tus - DNA replication terminus site-binding protein (NCBI), at 1,682,283 to 1,683,212
tus
Position (kb)
1680
1681
1682
1683 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1679.924 kb on - strand, within ydgC at 1680.147 kb on + strand at 1680.268 kb on + strand, within rstA at 1680.438 kb on + strand, within rstA at 1680.439 kb on + strand, within rstA at 1680.439 kb on + strand, within rstA at 1680.544 kb on + strand, within rstA at 1680.552 kb on - strand, within rstA at 1680.552 kb on - strand, within rstA at 1680.575 kb on + strand, within rstA at 1680.981 kb on + strand at 1680.981 kb on + strand at 1681.080 kb on + strand, within rstB at 1681.099 kb on - strand, within rstB at 1681.176 kb on + strand, within rstB at 1681.555 kb on + strand, within rstB at 1681.555 kb on + strand, within rstB at 1681.779 kb on - strand, within rstB at 1681.779 kb on - strand, within rstB at 1681.827 kb on + strand, within rstB at 1682.011 kb on + strand, within rstB at 1682.068 kb on + strand, within rstB at 1682.068 kb on + strand, within rstB at 1682.090 kb on + strand at 1682.098 kb on - strand at 1682.100 kb on - strand at 1682.102 kb on + strand at 1682.102 kb on + strand at 1682.223 kb on + strand at 1682.223 kb on + strand at 1682.306 kb on - strand at 1682.451 kb on - strand, within tus at 1682.567 kb on - strand, within tus at 1682.620 kb on + strand, within tus at 1682.620 kb on + strand, within tus at 1682.820 kb on + strand, within tus at 1682.838 kb on + strand, within tus at 1682.878 kb on + strand, within tus at 1682.886 kb on - strand, within tus at 1682.941 kb on - strand, within tus at 1682.941 kb on - strand, within tus at 1683.046 kb on + strand, within tus at 1683.046 kb on + strand, within tus at 1683.207 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 1,679,924 - ydgC b1607 0.61 -0.5 1,680,147 + +0.3 1,680,268 + rstA b1608 0.12 -0.8 1,680,438 + rstA b1608 0.35 +1.4 1,680,439 + rstA b1608 0.36 -0.3 1,680,439 + rstA b1608 0.36 -0.9 1,680,544 + rstA b1608 0.50 -0.5 1,680,552 - rstA b1608 0.51 -0.9 1,680,552 - rstA b1608 0.51 +0.1 1,680,575 + rstA b1608 0.54 +0.8 1,680,981 + +0.7 1,680,981 + +0.7 1,681,080 + rstB b1609 0.13 -0.4 1,681,099 - rstB b1609 0.15 +0.1 1,681,176 + rstB b1609 0.21 -1.5 1,681,555 + rstB b1609 0.50 -1.3 1,681,555 + rstB b1609 0.50 -0.9 1,681,779 - rstB b1609 0.67 -0.9 1,681,779 - rstB b1609 0.67 +0.1 1,681,827 + rstB b1609 0.71 +1.7 1,682,011 + rstB b1609 0.85 -0.5 1,682,068 + rstB b1609 0.89 -0.9 1,682,068 + rstB b1609 0.89 -1.2 1,682,090 + +0.8 1,682,098 - +0.1 1,682,100 - -0.7 1,682,102 + +0.1 1,682,102 + -0.9 1,682,223 + +0.7 1,682,223 + -2.5 1,682,306 - -0.5 1,682,451 - tus b1610 0.18 +0.2 1,682,567 - tus b1610 0.31 +2.1 1,682,620 + tus b1610 0.36 +1.1 1,682,620 + tus b1610 0.36 +1.1 1,682,820 + tus b1610 0.58 +1.4 1,682,838 + tus b1610 0.60 +0.9 1,682,878 + tus b1610 0.64 +0.6 1,682,886 - tus b1610 0.65 +1.5 1,682,941 - tus b1610 0.71 -0.9 1,682,941 - tus b1610 0.71 -0.7 1,683,046 + tus b1610 0.82 +0.1 1,683,046 + tus b1610 0.82 -1.5 1,683,207 + +0.1
Or see this region's nucleotide sequence