Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt speG and ynfC are separated by 2 nucleotides ynfC and b1586 are separated by 107 nucleotides b1586 and ynfE are separated by 198 nucleotides
b1584: speG - spermidine N1-acetyltransferase (NCBI), at 1,654,208 to 1,654,768
speG
b1585: ynfC - orf, hypothetical protein (VIMSS), at 1,654,771 to 1,655,481
ynfC
b1586: b1586 - orf, hypothetical protein (VIMSS), at 1,655,589 to 1,655,894
b1586
b1587: ynfE - oxidoreductase subunit (NCBI), at 1,656,093 to 1,658,519
ynfE
Position (kb)
1655
1656 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1654.690 kb on + strand, within speG at 1654.690 kb on + strand, within speG at 1654.711 kb on + strand, within speG at 1654.711 kb on + strand, within speG at 1654.719 kb on - strand at 1654.723 kb on + strand at 1654.723 kb on + strand at 1654.731 kb on - strand at 1654.745 kb on + strand at 1654.745 kb on + strand at 1654.753 kb on - strand at 1654.799 kb on + strand at 1654.807 kb on - strand at 1654.807 kb on - strand at 1654.812 kb on - strand at 1654.812 kb on - strand at 1655.022 kb on - strand, within ynfC at 1655.058 kb on + strand, within ynfC at 1655.077 kb on - strand, within ynfC at 1655.077 kb on - strand, within ynfC at 1655.102 kb on + strand, within ynfC at 1655.114 kb on + strand, within ynfC at 1655.123 kb on - strand, within ynfC at 1655.123 kb on - strand, within ynfC at 1655.147 kb on - strand, within ynfC at 1655.175 kb on - strand, within ynfC at 1655.199 kb on - strand, within ynfC at 1655.208 kb on + strand, within ynfC at 1655.216 kb on - strand, within ynfC at 1655.394 kb on + strand, within ynfC at 1655.402 kb on - strand at 1655.402 kb on - strand, within ynfC at 1655.413 kb on - strand at 1655.428 kb on + strand at 1655.500 kb on - strand at 1655.500 kb on - strand at 1655.626 kb on + strand, within b1586 at 1655.634 kb on - strand, within b1586 at 1655.643 kb on + strand, within b1586 at 1655.645 kb on + strand, within b1586 at 1655.655 kb on + strand, within b1586 at 1655.655 kb on + strand, within b1586 at 1655.786 kb on - strand, within b1586 at 1655.786 kb on - strand, within b1586 at 1655.841 kb on + strand, within b1586 at 1655.858 kb on + strand, within b1586 at 1655.955 kb on - strand at 1656.025 kb on - strand at 1656.291 kb on - strand at 1656.291 kb on - strand at 1656.418 kb on - strand, within ynfE at 1656.569 kb on - strand, within ynfE at 1656.673 kb on - strand, within ynfE at 1656.712 kb on - strand, within ynfE at 1656.712 kb on - strand, within ynfE at 1656.800 kb on + strand, within ynfE at 1656.800 kb on + strand, within ynfE
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 1,654,690 + speG b1584 0.86 +0.7 1,654,690 + speG b1584 0.86 +0.1 1,654,711 + speG b1584 0.90 -0.9 1,654,711 + speG b1584 0.90 -0.7 1,654,719 - +0.6 1,654,723 + +0.1 1,654,723 + +1.1 1,654,731 - +0.2 1,654,745 + +1.1 1,654,745 + +1.5 1,654,753 - +1.1 1,654,799 + +0.1 1,654,807 - +1.7 1,654,807 - +0.5 1,654,812 - +0.1 1,654,812 - +0.7 1,655,022 - ynfC b1585 0.35 +0.8 1,655,058 + ynfC b1585 0.40 -0.5 1,655,077 - ynfC b1585 0.43 -1.5 1,655,077 - ynfC b1585 0.43 -0.5 1,655,102 + ynfC b1585 0.47 -0.9 1,655,114 + ynfC b1585 0.48 +1.7 1,655,123 - ynfC b1585 0.50 +0.4 1,655,123 - ynfC b1585 0.50 +1.1 1,655,147 - ynfC b1585 0.53 -0.9 1,655,175 - ynfC b1585 0.57 -1.3 1,655,199 - ynfC b1585 0.60 -0.3 1,655,208 + ynfC b1585 0.61 +1.1 1,655,216 - ynfC b1585 0.63 +0.5 1,655,394 + ynfC b1585 0.88 +0.4 1,655,402 - +0.1 1,655,402 - ynfC b1585 0.89 +1.4 1,655,413 - +0.1 1,655,428 + -0.7 1,655,500 - +0.1 1,655,500 - +1.4 1,655,626 + b1586 b1586 0.12 +0.1 1,655,634 - b1586 b1586 0.15 +1.7 1,655,643 + b1586 b1586 0.18 +2.1 1,655,645 + b1586 b1586 0.18 -0.9 1,655,655 + b1586 b1586 0.22 +0.1 1,655,655 + b1586 b1586 0.22 -0.9 1,655,786 - b1586 b1586 0.64 +0.1 1,655,786 - b1586 b1586 0.64 +0.1 1,655,841 + b1586 b1586 0.82 +3.4 1,655,858 + b1586 b1586 0.88 +0.4 1,655,955 - +1.7 1,656,025 - +1.5 1,656,291 - +1.1 1,656,291 - +0.3 1,656,418 - ynfE b1587 0.13 -1.5 1,656,569 - ynfE b1587 0.20 -0.2 1,656,673 - ynfE b1587 0.24 +1.7 1,656,712 - ynfE b1587 0.26 +0.1 1,656,712 - ynfE b1587 0.26 -1.5 1,656,800 + ynfE b1587 0.29 +0.1 1,656,800 + ynfE b1587 0.29 -0.5
Or see this region's nucleotide sequence