Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt insH-5 and b1372 are separated by 322 nucleotides b1372 and tfaR overlap by 1 nucleotides tfaR and pinR are separated by 97 nucleotides
b1370: insH-5 - Rac prophage; IS5 transposase and trans-activator (NCBI), at 1,425,770 to 1,426,750
insH-5
b1372: b1372 - putative membrane protein (VIMSS), at 1,427,073 to 1,430,435
b1372
b1373: tfaR - Rac prophage; predicted tail fiber assembly protein (NCBI), at 1,430,435 to 1,431,010
tfaR
b1374: pinR - Rac prophage; predicted site-specific recombinase (NCBI), at 1,431,108 to 1,431,698
pinR
Position (kb)
1427
1428
1429
1430
1431 Strain fitness (log2 ratio)
-1
0
1
2 at 1426.405 kb on + strand, within insH-5 at 1426.456 kb on - strand, within insH-5 at 1426.658 kb on + strand at 1426.658 kb on + strand at 1426.738 kb on + strand at 1426.738 kb on + strand at 1426.746 kb on + strand at 1426.877 kb on + strand at 1426.954 kb on - strand at 1426.954 kb on - strand at 1427.144 kb on - strand at 1427.251 kb on - strand at 1427.764 kb on - strand, within b1372 at 1427.764 kb on - strand, within b1372 at 1427.937 kb on + strand, within b1372 at 1428.125 kb on + strand, within b1372 at 1428.482 kb on + strand, within b1372 at 1428.485 kb on - strand, within b1372 at 1428.485 kb on - strand, within b1372 at 1428.506 kb on + strand, within b1372 at 1428.633 kb on - strand, within b1372 at 1428.633 kb on - strand, within b1372 at 1428.681 kb on - strand, within b1372 at 1428.681 kb on - strand, within b1372 at 1428.751 kb on + strand, within b1372 at 1428.807 kb on - strand, within b1372 at 1428.972 kb on + strand, within b1372 at 1429.180 kb on - strand, within b1372 at 1429.180 kb on - strand, within b1372 at 1429.248 kb on + strand, within b1372 at 1429.418 kb on + strand, within b1372 at 1429.429 kb on + strand, within b1372 at 1429.429 kb on + strand, within b1372 at 1429.526 kb on - strand, within b1372 at 1429.542 kb on - strand, within b1372 at 1429.679 kb on + strand, within b1372 at 1429.832 kb on - strand, within b1372 at 1430.281 kb on + strand at 1431.253 kb on - strand, within pinR
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 1,426,405 + insH-5 b1370 0.65 +2.4 1,426,456 - insH-5 b1370 0.70 -0.5 1,426,658 + +0.1 1,426,658 + -0.2 1,426,738 + +0.1 1,426,738 + +0.1 1,426,746 + -0.5 1,426,877 + +0.4 1,426,954 - -0.9 1,426,954 - -0.9 1,427,144 - -0.3 1,427,251 - +0.2 1,427,764 - b1372 b1372 0.21 +1.1 1,427,764 - b1372 b1372 0.21 +0.1 1,427,937 + b1372 b1372 0.26 +0.8 1,428,125 + b1372 b1372 0.31 -0.2 1,428,482 + b1372 b1372 0.42 +1.7 1,428,485 - b1372 b1372 0.42 +1.7 1,428,485 - b1372 b1372 0.42 -0.6 1,428,506 + b1372 b1372 0.43 -0.1 1,428,633 - b1372 b1372 0.46 +1.5 1,428,633 - b1372 b1372 0.46 +0.7 1,428,681 - b1372 b1372 0.48 +0.1 1,428,681 - b1372 b1372 0.48 +1.1 1,428,751 + b1372 b1372 0.50 +1.1 1,428,807 - b1372 b1372 0.52 +0.7 1,428,972 + b1372 b1372 0.56 +0.3 1,429,180 - b1372 b1372 0.63 -0.5 1,429,180 - b1372 b1372 0.63 -0.9 1,429,248 + b1372 b1372 0.65 +0.1 1,429,418 + b1372 b1372 0.70 -0.9 1,429,429 + b1372 b1372 0.70 +1.4 1,429,429 + b1372 b1372 0.70 +0.1 1,429,526 - b1372 b1372 0.73 +0.7 1,429,542 - b1372 b1372 0.73 +1.4 1,429,679 + b1372 b1372 0.77 -0.1 1,429,832 - b1372 b1372 0.82 +0.4 1,430,281 + -0.5 1,431,253 - pinR b1374 0.25 -0.9
Or see this region's nucleotide sequence