Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rluB and btuR are separated by 39 nucleotides btuR and yciK overlap by 4 nucleotides yciK and sohB are separated by 219 nucleotides
b1269: rluB - 23S rRNA pseudouridylate synthase (NCBI), at 1,324,876 to 1,325,751
rluB
b1270: btuR - cob(I)yrinic acid a,c-diamide adenosyltransferase (NCBI), at 1,325,791 to 1,326,381
btuR
b1271: yciK - short chain dehydrogenase (NCBI), at 1,326,378 to 1,327,136
yciK
b1272: sohB - predicted inner membrane peptidase (NCBI), at 1,327,356 to 1,328,405
sohB
Position (kb)
1326
1327
1328 Strain fitness (log2 ratio)
-1
0
1 at 1325.635 kb on - strand, within rluB at 1325.636 kb on - strand, within rluB at 1325.636 kb on - strand, within rluB at 1325.814 kb on + strand at 1325.882 kb on - strand, within btuR at 1325.954 kb on - strand, within btuR at 1326.099 kb on - strand, within btuR at 1326.099 kb on - strand, within btuR at 1326.296 kb on - strand, within btuR at 1326.420 kb on + strand at 1326.420 kb on + strand at 1326.546 kb on + strand, within yciK at 1326.719 kb on + strand, within yciK at 1326.742 kb on - strand, within yciK at 1326.851 kb on - strand, within yciK at 1327.275 kb on + strand at 1327.297 kb on + strand at 1327.500 kb on + strand, within sohB at 1327.505 kb on + strand, within sohB at 1327.505 kb on + strand, within sohB at 1327.511 kb on - strand, within sohB at 1327.511 kb on - strand, within sohB at 1327.565 kb on + strand, within sohB at 1327.565 kb on + strand, within sohB at 1327.752 kb on - strand, within sohB at 1327.752 kb on - strand, within sohB at 1327.797 kb on + strand, within sohB at 1327.797 kb on + strand, within sohB at 1327.902 kb on - strand, within sohB at 1328.003 kb on + strand, within sohB at 1328.032 kb on - strand, within sohB at 1328.039 kb on + strand, within sohB at 1328.039 kb on + strand, within sohB
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 1,325,635 - rluB b1269 0.87 +0.8 1,325,636 - rluB b1269 0.87 +0.6 1,325,636 - rluB b1269 0.87 +1.1 1,325,814 + -0.1 1,325,882 - btuR b1270 0.15 +1.1 1,325,954 - btuR b1270 0.28 -0.6 1,326,099 - btuR b1270 0.52 -1.2 1,326,099 - btuR b1270 0.52 +0.1 1,326,296 - btuR b1270 0.85 +0.4 1,326,420 + +1.4 1,326,420 + +0.6 1,326,546 + yciK b1271 0.22 -0.9 1,326,719 + yciK b1271 0.45 +1.1 1,326,742 - yciK b1271 0.48 -1.7 1,326,851 - yciK b1271 0.62 -0.4 1,327,275 + +0.1 1,327,297 + -0.2 1,327,500 + sohB b1272 0.14 -1.0 1,327,505 + sohB b1272 0.14 +0.4 1,327,505 + sohB b1272 0.14 -0.7 1,327,511 - sohB b1272 0.15 +1.7 1,327,511 - sohB b1272 0.15 -0.9 1,327,565 + sohB b1272 0.20 -0.6 1,327,565 + sohB b1272 0.20 +0.7 1,327,752 - sohB b1272 0.38 +0.1 1,327,752 - sohB b1272 0.38 -0.4 1,327,797 + sohB b1272 0.42 -0.9 1,327,797 + sohB b1272 0.42 +0.1 1,327,902 - sohB b1272 0.52 -1.4 1,328,003 + sohB b1272 0.62 -0.9 1,328,032 - sohB b1272 0.64 -0.7 1,328,039 + sohB b1272 0.65 +0.1 1,328,039 + sohB b1272 0.65 -0.4
Or see this region's nucleotide sequence