Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prfA and prmC overlap by 1 nucleotides prmC and ychQ overlap by 4 nucleotides ychQ and ychA are separated by 3 nucleotides ychA and kdsA are separated by 35 nucleotides
b1211: prfA - peptide chain release factor 1 (NCBI), at 1,264,235 to 1,265,317
prfA
b1212: prmC - N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase (NCBI), at 1,265,317 to 1,266,150
prmC
b1213: ychQ - predicted transcriptional regulator (NCBI), at 1,266,147 to 1,266,539
ychQ
b1214: ychA - predicted transcriptional regulator (NCBI), at 1,266,543 to 1,267,352
ychA
b1215: kdsA - 2-dehydro-3-deoxyphosphooctonate aldolase (NCBI), at 1,267,388 to 1,268,242
kdsA
Position (kb)
1266
1267 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1266.145 kb on - strand at 1266.167 kb on + strand at 1266.172 kb on + strand at 1266.240 kb on - strand, within ychQ at 1266.325 kb on + strand, within ychQ at 1266.325 kb on + strand, within ychQ at 1266.435 kb on - strand, within ychQ at 1266.483 kb on + strand, within ychQ at 1266.522 kb on - strand at 1266.644 kb on - strand, within ychA at 1266.701 kb on + strand, within ychA at 1266.737 kb on + strand, within ychA at 1266.990 kb on + strand, within ychA at 1267.094 kb on + strand, within ychA at 1267.104 kb on - strand, within ychA at 1267.139 kb on + strand, within ychA at 1267.242 kb on + strand, within ychA at 1267.242 kb on + strand, within ychA at 1267.242 kb on + strand, within ychA at 1267.274 kb on + strand at 1267.274 kb on + strand at 1267.274 kb on - strand at 1267.276 kb on + strand at 1267.276 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 1,266,145 - +1.2 1,266,167 + -0.4 1,266,172 + -0.3 1,266,240 - ychQ b1213 0.24 -0.6 1,266,325 + ychQ b1213 0.45 -2.8 1,266,325 + ychQ b1213 0.45 -1.3 1,266,435 - ychQ b1213 0.73 +0.4 1,266,483 + ychQ b1213 0.85 -1.8 1,266,522 - +1.0 1,266,644 - ychA b1214 0.12 -0.8 1,266,701 + ychA b1214 0.20 -0.1 1,266,737 + ychA b1214 0.24 +0.2 1,266,990 + ychA b1214 0.55 -0.2 1,267,094 + ychA b1214 0.68 +1.2 1,267,104 - ychA b1214 0.69 -1.8 1,267,139 + ychA b1214 0.74 -1.0 1,267,242 + ychA b1214 0.86 +1.8 1,267,242 + ychA b1214 0.86 -0.2 1,267,242 + ychA b1214 0.86 -0.8 1,267,274 + -0.8 1,267,274 + +0.5 1,267,274 - -0.4 1,267,276 + +1.5 1,267,276 + -0.6
Or see this region's nucleotide sequence