Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ldcA and mltE are separated by 99 nucleotides mltE and ycgR are separated by 1 nucleotides ycgR and ymgE are separated by 200 nucleotides
b1192: ldcA - L,D-carboxypeptidase A (NCBI), at 1,241,389 to 1,242,303
ldcA
b1193: mltE - murein transglycosylase E (VIMSS), at 1,242,403 to 1,243,014
mltE
b1194: ycgR - protein involved in flagellar function (NCBI), at 1,243,016 to 1,243,750
ycgR
b1195: ymgE - predicted inner membrane protein (NCBI), at 1,243,951 to 1,244,205
ymgE
Position (kb)
1242
1243
1244 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1241.424 kb on + strand at 1241.425 kb on - strand at 1241.433 kb on + strand at 1241.501 kb on + strand, within ldcA at 1241.572 kb on - strand, within ldcA at 1241.581 kb on + strand, within ldcA at 1241.747 kb on + strand, within ldcA at 1241.936 kb on - strand, within ldcA at 1241.936 kb on - strand, within ldcA at 1241.961 kb on - strand, within ldcA at 1241.961 kb on - strand, within ldcA at 1241.999 kb on - strand, within ldcA at 1242.033 kb on + strand, within ldcA at 1242.208 kb on - strand, within ldcA at 1242.237 kb on - strand at 1242.284 kb on + strand at 1242.305 kb on - strand at 1242.337 kb on + strand at 1242.355 kb on - strand at 1242.365 kb on - strand at 1242.365 kb on - strand at 1242.465 kb on + strand, within mltE at 1242.655 kb on + strand, within mltE at 1243.159 kb on + strand, within ycgR at 1243.291 kb on - strand, within ycgR at 1243.291 kb on - strand, within ycgR at 1243.521 kb on + strand, within ycgR at 1243.589 kb on + strand, within ycgR at 1243.637 kb on - strand, within ycgR at 1243.688 kb on + strand at 1243.722 kb on - strand at 1243.732 kb on + strand at 1243.798 kb on + strand at 1243.864 kb on + strand at 1243.875 kb on + strand at 1243.890 kb on + strand at 1243.893 kb on - strand at 1243.899 kb on + strand at 1243.904 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 1,241,424 + +0.1 1,241,425 - +1.5 1,241,433 + +1.6 1,241,501 + ldcA b1192 0.12 +0.1 1,241,572 - ldcA b1192 0.20 -0.6 1,241,581 + ldcA b1192 0.21 +0.2 1,241,747 + ldcA b1192 0.39 +0.2 1,241,936 - ldcA b1192 0.60 +1.6 1,241,936 - ldcA b1192 0.60 +1.6 1,241,961 - ldcA b1192 0.63 +0.2 1,241,961 - ldcA b1192 0.63 +2.2 1,241,999 - ldcA b1192 0.67 +1.2 1,242,033 + ldcA b1192 0.70 +0.9 1,242,208 - ldcA b1192 0.90 -0.8 1,242,237 - +1.3 1,242,284 + +1.8 1,242,305 - -0.3 1,242,337 + +0.8 1,242,355 - +0.0 1,242,365 - -0.1 1,242,365 - +2.2 1,242,465 + mltE b1193 0.10 +0.2 1,242,655 + mltE b1193 0.41 +0.8 1,243,159 + ycgR b1194 0.19 +0.6 1,243,291 - ycgR b1194 0.37 -1.4 1,243,291 - ycgR b1194 0.37 -2.1 1,243,521 + ycgR b1194 0.69 -0.2 1,243,589 + ycgR b1194 0.78 -0.8 1,243,637 - ycgR b1194 0.84 +0.2 1,243,688 + +0.2 1,243,722 - -1.1 1,243,732 + +0.2 1,243,798 + +1.3 1,243,864 + +2.2 1,243,875 + +0.7 1,243,890 + +0.9 1,243,893 - +0.4 1,243,899 + +0.5 1,243,904 + +0.2
Or see this region's nucleotide sequence