Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fhuE and ycfF are separated by 333 nucleotides ycfF and ycfL are separated by 2 nucleotides ycfL and ycfM are separated by 13 nucleotides ycfM and ycfN overlap by 20 nucleotides
b1102: fhuE - ferric-rhodotorulic acid outer membrane transporter (NCBI), at 1,158,585 to 1,160,774
fhuE
b1103: ycfF - orf, hypothetical protein (VIMSS), at 1,161,108 to 1,161,467
ycfF
b1104: ycfL - hypothetical protein (NCBI), at 1,161,470 to 1,161,847
ycfL
b1105: ycfM - predicted outer membrane lipoprotein (NCBI), at 1,161,861 to 1,162,502
ycfM
b1106: ycfN - thiamin kinase (NCBI), at 1,162,483 to 1,163,307
ycfN
Position (kb)
1161
1162 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1160.735 kb on - strand at 1160.799 kb on + strand at 1161.023 kb on + strand at 1161.023 kb on + strand at 1161.034 kb on + strand at 1161.048 kb on - strand at 1161.048 kb on - strand at 1161.180 kb on - strand, within ycfF at 1161.414 kb on - strand, within ycfF at 1161.508 kb on - strand, within ycfL at 1161.648 kb on - strand, within ycfL at 1161.659 kb on + strand, within ycfL at 1161.996 kb on + strand, within ycfM at 1162.014 kb on + strand, within ycfM at 1162.014 kb on + strand, within ycfM at 1162.190 kb on - strand, within ycfM at 1162.347 kb on - strand, within ycfM at 1162.456 kb on - strand at 1162.640 kb on + strand, within ycfN
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 1,160,735 - -0.1 1,160,799 + -0.4 1,161,023 + -0.8 1,161,023 + -0.2 1,161,034 + -2.6 1,161,048 - +0.6 1,161,048 - -0.8 1,161,180 - ycfF b1103 0.20 +0.2 1,161,414 - ycfF b1103 0.85 -2.8 1,161,508 - ycfL b1104 0.10 -1.0 1,161,648 - ycfL b1104 0.47 -1.0 1,161,659 + ycfL b1104 0.50 -1.4 1,161,996 + ycfM b1105 0.21 -1.1 1,162,014 + ycfM b1105 0.24 +0.8 1,162,014 + ycfM b1105 0.24 +0.9 1,162,190 - ycfM b1105 0.51 +1.9 1,162,347 - ycfM b1105 0.76 +0.2 1,162,456 - +0.9 1,162,640 + ycfN b1106 0.19 +0.8
Or see this region's nucleotide sequence