Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flgI and flgJ overlap by 1 nucleotides flgJ and flgK are separated by 65 nucleotides
b1080: flgI - homolog of Salmonella P-ring of flagella basal body (VIMSS), at 1,135,497 to 1,136,594
flgI
b1081: flgJ - flagellar biosynthesis protein FlgJ (NCBI), at 1,136,594 to 1,137,535
flgJ
b1082: flgK - flagellar hook-associated protein K (NCBI), at 1,137,601 to 1,139,244
flgK
Position (kb)
1136
1137
1138 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1135.634 kb on - strand, within flgI at 1135.838 kb on + strand, within flgI at 1135.849 kb on + strand, within flgI at 1135.867 kb on - strand, within flgI at 1135.913 kb on - strand, within flgI at 1136.103 kb on + strand, within flgI at 1136.129 kb on - strand, within flgI at 1136.129 kb on - strand, within flgI at 1136.165 kb on - strand, within flgI at 1136.168 kb on + strand, within flgI at 1136.312 kb on - strand, within flgI at 1136.312 kb on - strand, within flgI at 1136.319 kb on + strand, within flgI at 1136.335 kb on - strand, within flgI at 1136.399 kb on + strand, within flgI at 1136.413 kb on - strand, within flgI at 1136.451 kb on + strand, within flgI at 1136.507 kb on + strand at 1136.507 kb on + strand at 1136.620 kb on - strand at 1136.622 kb on - strand at 1136.622 kb on - strand at 1136.776 kb on + strand, within flgJ at 1136.776 kb on + strand, within flgJ at 1136.810 kb on + strand, within flgJ at 1136.810 kb on + strand, within flgJ at 1136.821 kb on + strand, within flgJ at 1136.854 kb on - strand, within flgJ at 1136.867 kb on - strand, within flgJ at 1136.957 kb on - strand, within flgJ at 1137.015 kb on - strand, within flgJ at 1137.015 kb on - strand, within flgJ at 1137.122 kb on - strand, within flgJ at 1137.373 kb on - strand, within flgJ at 1137.452 kb on + strand at 1137.684 kb on + strand at 1137.691 kb on - strand at 1137.691 kb on - strand at 1137.705 kb on - strand at 1137.740 kb on - strand at 1138.112 kb on - strand, within flgK at 1138.255 kb on + strand, within flgK at 1138.306 kb on + strand, within flgK at 1138.350 kb on + strand, within flgK at 1138.362 kb on + strand, within flgK at 1138.369 kb on + strand, within flgK at 1138.422 kb on + strand, within flgK at 1138.422 kb on + strand, within flgK at 1138.469 kb on + strand, within flgK at 1138.530 kb on - strand, within flgK
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 1,135,634 - flgI b1080 0.12 +0.5 1,135,838 + flgI b1080 0.31 -0.1 1,135,849 + flgI b1080 0.32 +0.2 1,135,867 - flgI b1080 0.34 +1.2 1,135,913 - flgI b1080 0.38 -0.4 1,136,103 + flgI b1080 0.55 -0.6 1,136,129 - flgI b1080 0.58 -0.4 1,136,129 - flgI b1080 0.58 -1.8 1,136,165 - flgI b1080 0.61 +1.8 1,136,168 + flgI b1080 0.61 +1.3 1,136,312 - flgI b1080 0.74 +0.7 1,136,312 - flgI b1080 0.74 -0.6 1,136,319 + flgI b1080 0.75 -0.2 1,136,335 - flgI b1080 0.76 +1.6 1,136,399 + flgI b1080 0.82 +0.8 1,136,413 - flgI b1080 0.83 -0.8 1,136,451 + flgI b1080 0.87 -0.1 1,136,507 + +0.4 1,136,507 + +0.0 1,136,620 - -1.4 1,136,622 - -0.0 1,136,622 - +0.8 1,136,776 + flgJ b1081 0.19 +0.7 1,136,776 + flgJ b1081 0.19 +1.0 1,136,810 + flgJ b1081 0.23 +0.2 1,136,810 + flgJ b1081 0.23 +1.9 1,136,821 + flgJ b1081 0.24 +0.9 1,136,854 - flgJ b1081 0.28 +0.9 1,136,867 - flgJ b1081 0.29 -0.5 1,136,957 - flgJ b1081 0.39 +0.6 1,137,015 - flgJ b1081 0.45 +0.2 1,137,015 - flgJ b1081 0.45 +1.2 1,137,122 - flgJ b1081 0.56 -0.5 1,137,373 - flgJ b1081 0.83 +0.5 1,137,452 + -0.1 1,137,684 + -0.4 1,137,691 - +0.8 1,137,691 - +0.2 1,137,705 - +0.2 1,137,740 - +0.2 1,138,112 - flgK b1082 0.31 -0.8 1,138,255 + flgK b1082 0.40 +0.2 1,138,306 + flgK b1082 0.43 -0.8 1,138,350 + flgK b1082 0.46 +0.2 1,138,362 + flgK b1082 0.46 -0.4 1,138,369 + flgK b1082 0.47 +0.6 1,138,422 + flgK b1082 0.50 +0.2 1,138,422 + flgK b1082 0.50 +0.4 1,138,469 + flgK b1082 0.53 +0.9 1,138,530 - flgK b1082 0.57 -0.0
Or see this region's nucleotide sequence