Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybjP and ybjQ are separated by 125 nucleotides ybjQ and ybjR overlap by 4 nucleotides ybjR and b0868 overlap by 4 nucleotides
b0865: ybjP - predicted lipoprotein (NCBI), at 903,175 to 903,690
ybjP
b0866: ybjQ - hypothetical protein (NCBI), at 903,816 to 904,139
ybjQ
b0867: ybjR - predicted amidase and lipoprotein (NCBI), at 904,136 to 904,966
ybjR
b0868: b0868 - putative nucleotide di-P-sugar epimerase or dehydratase (VIMSS), at 904,963 to 905,976
b0868
Position (kb) 904
905 Strain fitness (log2 ratio) -2
-1
0
1
2 at 903.275 kb on + strand, within ybjP at 903.299 kb on + strand, within ybjP at 903.473 kb on + strand, within ybjP at 903.473 kb on + strand, within ybjP at 903.597 kb on - strand, within ybjP at 903.597 kb on - strand, within ybjP at 903.641 kb on + strand at 903.651 kb on + strand at 903.695 kb on - strand at 903.842 kb on + strand at 904.093 kb on + strand, within ybjQ at 904.156 kb on + strand at 904.183 kb on + strand at 904.271 kb on - strand, within ybjR at 904.282 kb on + strand, within ybjR at 904.298 kb on + strand, within ybjR at 904.352 kb on - strand, within ybjR at 904.465 kb on + strand, within ybjR at 904.477 kb on - strand, within ybjR at 904.477 kb on - strand, within ybjR at 904.479 kb on + strand, within ybjR at 904.558 kb on + strand, within ybjR at 904.597 kb on - strand, within ybjR at 904.765 kb on - strand, within ybjR at 904.775 kb on - strand, within ybjR at 904.812 kb on - strand, within ybjR at 904.812 kb on - strand, within ybjR at 904.812 kb on - strand, within ybjR at 904.824 kb on + strand, within ybjR at 904.830 kb on + strand, within ybjR at 904.830 kb on + strand, within ybjR at 904.838 kb on - strand, within ybjR at 904.935 kb on + strand at 904.968 kb on - strand at 905.027 kb on - strand at 905.245 kb on + strand, within b0868 at 905.246 kb on - strand, within b0868 at 905.246 kb on - strand, within b0868 at 905.248 kb on + strand, within b0868 at 905.253 kb on + strand, within b0868 at 905.261 kb on - strand, within b0868 at 905.261 kb on - strand, within b0868 at 905.275 kb on - strand, within b0868 at 905.278 kb on + strand, within b0868 at 905.278 kb on + strand, within b0868 at 905.284 kb on - strand, within b0868 at 905.286 kb on - strand, within b0868 at 905.357 kb on - strand, within b0868 at 905.357 kb on - strand, within b0868 at 905.376 kb on - strand, within b0868 at 905.402 kb on + strand, within b0868 at 905.468 kb on + strand, within b0868 at 905.489 kb on - strand, within b0868 at 905.525 kb on - strand, within b0868 at 905.543 kb on + strand, within b0868 at 905.734 kb on + strand, within b0868 at 905.734 kb on + strand, within b0868 at 905.735 kb on + strand, within b0868 at 905.735 kb on + strand, within b0868 at 905.746 kb on + strand, within b0868 at 905.964 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 903,275 + ybjP b0865 0.19 +0.2 903,299 + ybjP b0865 0.24 +0.6 903,473 + ybjP b0865 0.58 -0.8 903,473 + ybjP b0865 0.58 +1.2 903,597 - ybjP b0865 0.82 -1.8 903,597 - ybjP b0865 0.82 -2.1 903,641 + -0.4 903,651 + +0.7 903,695 - +1.2 903,842 + -2.1 904,093 + ybjQ b0866 0.85 +0.6 904,156 + +0.2 904,183 + +0.8 904,271 - ybjR b0867 0.16 +0.2 904,282 + ybjR b0867 0.18 +1.2 904,298 + ybjR b0867 0.19 +0.0 904,352 - ybjR b0867 0.26 -0.2 904,465 + ybjR b0867 0.40 +0.6 904,477 - ybjR b0867 0.41 -0.8 904,477 - ybjR b0867 0.41 -0.2 904,479 + ybjR b0867 0.41 -1.8 904,558 + ybjR b0867 0.51 -0.2 904,597 - ybjR b0867 0.55 -0.1 904,765 - ybjR b0867 0.76 +0.2 904,775 - ybjR b0867 0.77 +0.5 904,812 - ybjR b0867 0.81 -0.5 904,812 - ybjR b0867 0.81 -0.7 904,812 - ybjR b0867 0.81 +0.6 904,824 + ybjR b0867 0.83 -1.1 904,830 + ybjR b0867 0.84 +0.2 904,830 + ybjR b0867 0.84 +0.2 904,838 - ybjR b0867 0.84 +0.4 904,935 + +0.8 904,968 - +0.9 905,027 - -0.5 905,245 + b0868 b0868 0.28 +1.8 905,246 - b0868 b0868 0.28 +0.8 905,246 - b0868 b0868 0.28 +1.5 905,248 + b0868 b0868 0.28 -1.8 905,253 + b0868 b0868 0.29 -0.3 905,261 - b0868 b0868 0.29 -1.1 905,261 - b0868 b0868 0.29 -0.1 905,275 - b0868 b0868 0.31 +1.3 905,278 + b0868 b0868 0.31 -0.5 905,278 + b0868 b0868 0.31 +0.4 905,284 - b0868 b0868 0.32 +0.8 905,286 - b0868 b0868 0.32 +1.5 905,357 - b0868 b0868 0.39 +0.8 905,357 - b0868 b0868 0.39 -2.2 905,376 - b0868 b0868 0.41 +0.2 905,402 + b0868 b0868 0.43 +0.5 905,468 + b0868 b0868 0.50 -0.9 905,489 - b0868 b0868 0.52 +1.1 905,525 - b0868 b0868 0.55 -0.5 905,543 + b0868 b0868 0.57 -0.4 905,734 + b0868 b0868 0.76 -1.4 905,734 + b0868 b0868 0.76 +0.7 905,735 + b0868 b0868 0.76 +1.7 905,735 + b0868 b0868 0.76 -1.4 905,746 + b0868 b0868 0.77 +0.9 905,964 + +0.7
Or see this region's nucleotide sequence