Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yliI and yliJ overlap by 4 nucleotides yliJ and dacC are separated by 246 nucleotides dacC and deoR are separated by 46 nucleotides deoR and ybjG are separated by 57 nucleotides
b0837: yliI - predicted dehydrogenase (NCBI), at 877,965 to 879,080
yliI
b0838: yliJ - putative transferase (VIMSS), at 879,077 to 879,703
yliJ
b0839: dacC - D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 6a) (NCBI), at 879,950 to 881,152
dacC
b0840: deoR - DNA-binding transcriptional repressor (NCBI), at 881,199 to 881,957
deoR
b0841: ybjG - undecaprenyl pyrophosphate phosphatase (NCBI), at 882,015 to 882,611
ybjG
Position (kb) 879
880
881
882 Strain fitness (log2 ratio) -2
-1
0
1
2 at 879.008 kb on + strand at 879.026 kb on + strand at 879.046 kb on - strand at 879.046 kb on - strand at 879.084 kb on - strand at 879.092 kb on + strand at 879.138 kb on - strand at 879.167 kb on + strand, within yliJ at 879.170 kb on - strand, within yliJ at 879.226 kb on - strand, within yliJ at 879.226 kb on - strand, within yliJ at 879.353 kb on - strand, within yliJ at 879.353 kb on - strand, within yliJ at 879.523 kb on - strand, within yliJ at 879.525 kb on - strand, within yliJ at 879.599 kb on - strand, within yliJ at 879.599 kb on - strand, within yliJ at 879.613 kb on - strand, within yliJ at 879.637 kb on + strand, within yliJ at 879.680 kb on + strand at 879.703 kb on + strand at 879.727 kb on + strand at 879.727 kb on + strand at 879.741 kb on - strand at 879.742 kb on - strand at 879.817 kb on - strand at 879.961 kb on - strand at 879.963 kb on + strand at 880.059 kb on - strand at 880.156 kb on + strand, within dacC at 880.170 kb on - strand, within dacC at 880.270 kb on - strand, within dacC at 880.298 kb on + strand, within dacC at 880.298 kb on + strand, within dacC at 880.306 kb on + strand, within dacC at 880.453 kb on + strand, within dacC at 880.472 kb on - strand, within dacC at 880.489 kb on + strand, within dacC at 880.524 kb on - strand, within dacC at 880.550 kb on + strand, within dacC at 880.550 kb on + strand, within dacC at 880.568 kb on + strand, within dacC at 880.676 kb on + strand, within dacC at 880.829 kb on + strand, within dacC at 880.829 kb on + strand, within dacC at 880.917 kb on + strand, within dacC at 880.917 kb on + strand, within dacC at 881.000 kb on + strand, within dacC at 881.094 kb on + strand at 881.094 kb on + strand at 881.146 kb on - strand at 881.159 kb on + strand at 881.228 kb on - strand at 881.283 kb on - strand, within deoR at 881.294 kb on - strand, within deoR at 881.371 kb on + strand, within deoR at 881.497 kb on + strand, within deoR at 881.588 kb on - strand, within deoR at 881.661 kb on - strand, within deoR at 881.765 kb on + strand, within deoR at 881.778 kb on - strand, within deoR at 881.778 kb on - strand, within deoR at 881.829 kb on + strand, within deoR at 881.878 kb on + strand, within deoR at 881.878 kb on + strand, within deoR at 881.902 kb on - strand at 881.906 kb on + strand at 881.906 kb on + strand at 881.957 kb on - strand at 881.957 kb on - strand at 882.047 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 879,008 + -0.3 879,026 + -0.6 879,046 - +0.5 879,046 - +0.1 879,084 - -1.4 879,092 + -0.7 879,138 - -1.5 879,167 + yliJ b0838 0.14 -0.5 879,170 - yliJ b0838 0.15 -0.8 879,226 - yliJ b0838 0.24 -0.4 879,226 - yliJ b0838 0.24 -0.5 879,353 - yliJ b0838 0.44 +0.8 879,353 - yliJ b0838 0.44 +0.2 879,523 - yliJ b0838 0.71 +0.5 879,525 - yliJ b0838 0.71 -0.1 879,599 - yliJ b0838 0.83 +0.2 879,599 - yliJ b0838 0.83 -1.2 879,613 - yliJ b0838 0.85 -0.1 879,637 + yliJ b0838 0.89 -0.8 879,680 + +0.1 879,703 + +2.2 879,727 + +0.2 879,727 + +1.5 879,741 - -0.8 879,742 - -0.5 879,817 - -0.8 879,961 - +0.1 879,963 + +0.2 880,059 - +0.8 880,156 + dacC b0839 0.17 -0.2 880,170 - dacC b0839 0.18 +1.0 880,270 - dacC b0839 0.27 +0.6 880,298 + dacC b0839 0.29 -0.3 880,298 + dacC b0839 0.29 +1.1 880,306 + dacC b0839 0.30 -0.1 880,453 + dacC b0839 0.42 +1.0 880,472 - dacC b0839 0.43 +0.2 880,489 + dacC b0839 0.45 +0.3 880,524 - dacC b0839 0.48 +0.7 880,550 + dacC b0839 0.50 +1.2 880,550 + dacC b0839 0.50 -1.4 880,568 + dacC b0839 0.51 +1.8 880,676 + dacC b0839 0.60 +0.8 880,829 + dacC b0839 0.73 +0.5 880,829 + dacC b0839 0.73 -0.8 880,917 + dacC b0839 0.80 +1.8 880,917 + dacC b0839 0.80 -1.4 881,000 + dacC b0839 0.87 -1.4 881,094 + +2.5 881,094 + +1.8 881,146 - +1.2 881,159 + -0.0 881,228 - +1.2 881,283 - deoR b0840 0.11 -0.4 881,294 - deoR b0840 0.13 -0.4 881,371 + deoR b0840 0.23 +0.8 881,497 + deoR b0840 0.39 -1.8 881,588 - deoR b0840 0.51 +0.5 881,661 - deoR b0840 0.61 +0.2 881,765 + deoR b0840 0.75 +0.2 881,778 - deoR b0840 0.76 +1.5 881,778 - deoR b0840 0.76 -0.8 881,829 + deoR b0840 0.83 -0.8 881,878 + deoR b0840 0.89 -0.5 881,878 + deoR b0840 0.89 -0.4 881,902 - -0.0 881,906 + +0.2 881,906 + -0.8 881,957 - -0.8 881,957 - -0.6 882,047 + +0.1
Or see this region's nucleotide sequence