Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kdpD and kdpC overlap by 8 nucleotides kdpC and kdpB are separated by 8 nucleotides
b0695: kdpD - fused sensory histidine kinase in two-component regulatory system with KdpE: signal sensing protein (NCBI), at 720,953 to 723,637
kdpD
b0696: kdpC - potassium-transporting ATPase subunit C (NCBI), at 723,630 to 724,202
kdpC
b0697: kdpB - potassium-transporting ATPase subunit B (NCBI), at 724,211 to 726,259
kdpB
Position (kb)
723
724
725 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 722.630 kb on + strand, within kdpD at 722.673 kb on + strand, within kdpD at 722.762 kb on + strand, within kdpD at 722.762 kb on + strand, within kdpD at 722.762 kb on + strand, within kdpD at 722.769 kb on + strand, within kdpD at 722.769 kb on + strand, within kdpD at 722.812 kb on - strand, within kdpD at 722.812 kb on - strand, within kdpD at 723.084 kb on + strand, within kdpD at 723.162 kb on - strand, within kdpD at 723.184 kb on - strand, within kdpD at 723.184 kb on - strand, within kdpD at 723.323 kb on - strand, within kdpD at 723.323 kb on - strand, within kdpD at 723.351 kb on - strand, within kdpD at 723.596 kb on + strand at 723.658 kb on - strand at 723.961 kb on - strand, within kdpC at 723.961 kb on - strand, within kdpC at 724.046 kb on - strand, within kdpC at 724.060 kb on + strand, within kdpC at 724.198 kb on + strand at 724.216 kb on - strand at 724.216 kb on - strand at 724.234 kb on + strand at 724.234 kb on + strand at 724.279 kb on + strand at 724.315 kb on - strand at 724.467 kb on - strand, within kdpB at 724.467 kb on - strand, within kdpB at 724.546 kb on - strand, within kdpB at 724.674 kb on + strand, within kdpB at 724.704 kb on - strand, within kdpB at 724.800 kb on + strand, within kdpB at 724.972 kb on - strand, within kdpB at 725.134 kb on + strand, within kdpB at 725.140 kb on - strand, within kdpB
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 722,630 + kdpD b0695 0.62 +1.2 722,673 + kdpD b0695 0.64 -0.4 722,762 + kdpD b0695 0.67 -0.4 722,762 + kdpD b0695 0.67 -0.0 722,762 + kdpD b0695 0.67 +0.5 722,769 + kdpD b0695 0.68 +1.2 722,769 + kdpD b0695 0.68 +0.5 722,812 - kdpD b0695 0.69 +0.8 722,812 - kdpD b0695 0.69 -1.0 723,084 + kdpD b0695 0.79 +0.0 723,162 - kdpD b0695 0.82 -0.5 723,184 - kdpD b0695 0.83 -0.8 723,184 - kdpD b0695 0.83 -1.4 723,323 - kdpD b0695 0.88 -0.5 723,323 - kdpD b0695 0.88 -0.8 723,351 - kdpD b0695 0.89 +0.2 723,596 + -0.2 723,658 - +0.2 723,961 - kdpC b0696 0.58 -0.0 723,961 - kdpC b0696 0.58 -0.8 724,046 - kdpC b0696 0.73 -2.9 724,060 + kdpC b0696 0.75 +0.2 724,198 + +0.0 724,216 - +0.6 724,216 - -0.5 724,234 + +0.7 724,234 + -1.5 724,279 + -1.1 724,315 - -0.9 724,467 - kdpB b0697 0.12 +0.2 724,467 - kdpB b0697 0.12 +0.2 724,546 - kdpB b0697 0.16 +0.6 724,674 + kdpB b0697 0.23 +0.9 724,704 - kdpB b0697 0.24 +0.7 724,800 + kdpB b0697 0.29 -0.5 724,972 - kdpB b0697 0.37 +0.7 725,134 + kdpB b0697 0.45 -0.5 725,140 - kdpB b0697 0.45 -2.6
Or see this region's nucleotide sequence