Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt citT and citG are separated by 50 nucleotides citG and citX overlap by 26 nucleotides citX and citF are separated by 3 nucleotides
b0612: citT - citrate:succinate antiporter (NCBI), at 644,340 to 645,803
citT
b0613: citG - triphosphoribosyl-dephospho-CoA transferase (NCBI), at 645,854 to 646,732
citG
b0614: citX - 2'-(5'-triphosphoribosyl)-3'-dephospho-CoA:apo- citrate lyase (NCBI), at 646,707 to 647,258
citX
b0615: citF - citrate lyase, citrate-ACP transferase (alpha) subunit (NCBI), at 647,262 to 648,794
citF
Position (kb) 646
647
648 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 645.720 kb on + strand at 645.800 kb on + strand at 645.889 kb on + strand at 645.968 kb on + strand, within citG at 646.007 kb on + strand, within citG at 646.007 kb on + strand, within citG at 646.019 kb on + strand, within citG at 646.132 kb on + strand, within citG at 646.308 kb on - strand, within citG at 646.308 kb on - strand, within citG at 646.530 kb on - strand, within citG at 646.595 kb on + strand, within citG at 646.595 kb on + strand, within citG at 646.656 kb on + strand at 646.666 kb on + strand at 646.666 kb on + strand at 646.675 kb on - strand at 646.675 kb on - strand at 647.268 kb on - strand at 647.354 kb on + strand at 647.383 kb on - strand at 647.428 kb on + strand, within citF at 647.428 kb on + strand, within citF at 647.522 kb on + strand, within citF at 647.601 kb on + strand, within citF at 648.197 kb on + strand, within citF at 648.197 kb on + strand, within citF
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 645,720 + +2.6 645,800 + +0.0 645,889 + -0.1 645,968 + citG b0613 0.13 -0.8 646,007 + citG b0613 0.17 -1.8 646,007 + citG b0613 0.17 -0.8 646,019 + citG b0613 0.19 -0.9 646,132 + citG b0613 0.32 -0.0 646,308 - citG b0613 0.52 -0.3 646,308 - citG b0613 0.52 +0.8 646,530 - citG b0613 0.77 -1.0 646,595 + citG b0613 0.84 +0.8 646,595 + citG b0613 0.84 -2.9 646,656 + +0.2 646,666 + -1.3 646,666 + -1.3 646,675 - -1.3 646,675 - +0.2 647,268 - +0.4 647,354 + +0.6 647,383 - +1.2 647,428 + citF b0615 0.11 -0.0 647,428 + citF b0615 0.11 -0.1 647,522 + citF b0615 0.17 +0.6 647,601 + citF b0615 0.22 -0.1 648,197 + citF b0615 0.61 -0.0 648,197 + citF b0615 0.61 -0.8
Or see this region's nucleotide sequence